We narrowed to 54,294 results for: Ran

1. pMK-mPrrn (5-fold decrease transcription)

   Plasmid

   #123478

   Purpose

   MoChlo kit

   Depositor

   C. Neal Stewart, Scott Lenaghan

   Article

   Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

   Insert

   mPrrn (5-fold decrease transcription)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGL4.23-IL2RA CaRE2 (minus strand)

   Plasmid

   #91847

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0624)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE2 (minus strand) (IL2RA Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   rs6602425 A>C, G>A mutation at chr10:611706…

   Promoter

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL4.23-CD69 CaRE1 (minus strand)

   Plasmid

   #91842

   Purpose

   Luciferase reporter for CD69 enhancer (IGI-P0619)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   CD69 CaRE1 (minus strand) (CD69 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Perfect match to reference sequence

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGL4.23-IL2RA CaRE6 (minus strand)

   Plasmid

   #91840

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0617)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE6 (minus strand) (IL2RA Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   rs7893467 C>A

   Promoter

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL4.23-IL2RA CaRE6 (plus strand)

   Plasmid

   #91839

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0616)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE6 (plus strand) (IL2RA Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   rs7893467 G>T, rs11256448 A>G

   Promoter

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGL4.23-IL2RA CaRE1 (minus strand)

   Plasmid

   #91838

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0615)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE1 (minus strand) (IL2RA Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Perfect match to reference sequence

   Promoter

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGL4.23-CD69 CaRE2 (plus strand)

   Plasmid

   #91848

   Purpose

   Luciferase reporter for CD69 enhancer (IGI-P0625)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   CD69 CaRE2 (plus strand) (CD69 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Perfect match to reference sequence

   Promoter

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL4.23-CD69 CaRE2 (minus strand)

   Plasmid

   #91843

   Purpose

   Luciferase reporter for CD69 enhancer (IGI-P0620)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   CD69 CaRE2 (minus strand) (CD69 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Perfect match to reference sequence

   Promoter

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCIneoRL-RanBP93'UTR-mir200c-mut

   Plasmid

   #84592

   Purpose

   Renilla Luciferase reporter assay for RANBP9 3'UTR with mutations on miR-200c binding site

   Depositor

   Yanan Yang

   Article

   Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

   Insert

   RANBP9 3'UTR (RANBP9 Human)
   

   Use

   Luciferase

   Tags

   Renilla Luciferase

   Expression

   Mutation

   Mutation on miR-200c binding site on RANBP9 3…

   Promoter

   CMV

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pGEX-SIRPT1-Xenopus (Silurana) tropicalis

    Plasmid

    #75323

    Purpose

    GST fusion Xenopus (Silurana) tropicalis Sacsin 87-1376

    Depositor

    Kalle Gehring

    Insert

    Sacsin Xenopus (Silurana) tropicalis 87-1376
    

    Use

    Tags

    GST-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p141 pETF-GST RanBP10 G308R

    Plasmid

    #11094

    Depositor

    Ramesh Shivdasani

    Insert

    RanBP10 (Ranbp10 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G308R

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

    Plasmid

    #128604

    Purpose

    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Inserts

    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pcmv

    Available since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFUGW ORANGE Gria1-GFP KI _ mCherry-KASH

    Plasmid

    #131507

    Purpose

    Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Gria1, Gria1 Rat, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUBC

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HA-MAO-N Mitochondrial Membrane-Targeting Cloning Vector

    Plasmid

    #139015

    Purpose

    To express a protein of interest fused to the N-terminus of HA-MAO for mitochondrial outer membrane targeting

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    HA-MAO

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP

    Plasmid

    #185686

    Purpose

    Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-GST-HaloTag-KKMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

    Plasmid

    #128755

    Purpose

    Expresses CYRANO gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    CYRANO sgRNA (OIP5-AS1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX408 Francisella tularensis subsp. novicida Cas9

    Plasmid

    #68705

    Purpose

    Francisella tularensis subsp. novicida Cas9

    Depositor

    Feng Zhang

    Insert

    FtCas9
    

    Use

    CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. delta PE OCT4-GFP-2A-PURO transgenic reporter

    Plasmid

    #52380

    Purpose

    BAC recombineering generated construct, used as a reporter for OCT4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP-2A-puro
    OCT4 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted -1620 - -1011 upstream from OCT4 ATG

    Promoter

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)

    Plasmid

    #51656

    Purpose

    hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrange

    Depositor

    Adam Cohen

    Insert

    Quasar2-mOrange-Chr64-GFP
    

    Use

    Lentiviral

    Tags

    GFP and mOrange

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. delta DE OCT4-GFP-2A-PURO transgenic reporter

    Plasmid

    #52381

    Purpose

    BAC recombineering generated construct, used as a reporter for OCT4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP-2A-puro
    OCT4 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted -3194 - -1768 upstream from OCT4 ATG

    Promoter

    OCT4

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFUGW ORANGE Tubb3-GFP KI _ mCherry-KASH

    Plasmid

    #131508

    Purpose

    Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUBC

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mouse deltaPE Oct4-GFP transgenic reporter

    Plasmid

    #52382

    Purpose

    BAC recombineering generated construct, used as a reporter for mouse Oct4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP pA
    Oct4 (Pou5f1 Mouse)
    PGK-gb2-Neo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted -300 - -1300bp fragment upstream from Oct…

    Promoter

    Oct4 and PGK+gb2

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGL4.23-IL2RA CaRE4 scramble (plus strand)

    Plasmid

    #91852

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0347)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 scramble (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Sequence scrambled from ch10:6094588-6094934

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mouse Oct4-GFP GOF18 transgenic reporter

    Plasmid

    #60527

    Purpose

    BAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    GFPpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Oct4

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid

    #116955

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMOS010: CheRiff-eGFP channelrhodopsin (plasma membrane)

    Plasmid

    #163044

    Purpose

    CheRiff-eGFP channelrhodopsin (plasma membrane)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    CheRiff
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTransgenesis p3 sv40 minimal promoter:: Katushka RFP

    Plasmid

    #131731

    Purpose

    Transposon based transgenesis

    Depositor

    Rene Maehr

    Article

    Stanney W et al Dev Biol. 2020 Mar 15;459(2):161-180. do

    Insert

    SV40MinPromoter
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

    Plasmid

    #185687

    Purpose

    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-GST-2xHaloTag-KKMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRK5 FLAG membrane-bound lipin-1 (TMD)

    Plasmid

    #120277

    Purpose

    Expression of ER/Golgi-tethered lipin-1 in mammalian cells

    Depositor

    Sirio Dupont

    Article

    Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

    Insert

    Lipin-1 (Lpin1 Mouse)
    

    Use

    Tags

    FLAG and in-frame fusion with the TMD Golgi-local…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Nuc-Orange Nano-lantern(Ca2+)/pcDNA3

    Plasmid

    #71349

    Purpose

    Express Nuc-Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern(Ca2+)_H2B
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

31. pGL4.23-IL2RA CaRE4 SNP (plus strand)

    Plasmid

    #91851

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0346)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 SNP (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    rs61839660 C>T

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHDR-CDKN2A-Ex2-CMV-H2B-mOrange2

    Plasmid

    #110735

    Purpose

    Recombination template for replacing CDKN2A Exon2 with Ef1alpha-H2B-mOrange2

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    H2B-mOrange2
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFS_0272_pET30_pCat-tetR-Term-ptetA-Rluc-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid

    #116956

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-Frankenbody-HA-mScarlet3-IRESv4-HaloTag-tdMCP

    Plasmid

    #241135

    Purpose

    Expresses Anti-HA frankenbody-mScarlet3 and HaloTag-tdMCP to track mature and nascent HA-tagged proteins and MS2-tagged mRNA

    Depositor

    Tim Stasevich

    Article

    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

    Inserts

    Anti-HA frankenbody
    tdMCP
    

    Use

    Tags

    HaloTag and mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PB-H2A-LSSmOrange2-2A-ERK-KTR-LSS-SGFP2

    Plasmid

    #129629

    Purpose

    Visualization of ERK activity

    Depositor

    Dorus Gadella

    Insert

    PB-H2A-LSSmOrange2-2A-ERK-KTR-LSS-SGFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLenti-CMV-FLAG-orangutan cGASdeltaN-OMM

    Plasmid

    #186889

    Purpose

    Expression of orangutan cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Orangutan cGAS truncation mutant (158-521 a.a.) with N-terminal FLAG tag and C-terminal MAVS transmembrane domain fusion (CGAS Synthetic, Pongo abelii)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    N-terminal truncation, MAVS transmembrane domain …

    Promoter

    CMV

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p23-NES-RanCas13b-msfGFP-NES-Flag

    Plasmid

    #165073

    Purpose

    overexpression of RanCas13b in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    RanCas13b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRK5 FLAG membrane-bound lipin-1 (delta200)

    Plasmid

    #120278

    Purpose

    Expression of ER/Golgi-tethered lipin-1 in mammalian cells

    Depositor

    Sirio Dupont

    Article

    Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

    Insert

    Lipin-1 (Lpin1 Mouse)
    

    Use

    Tags

    FLAG and in-frame fusion with the delta200 Golgi-…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Cre-responsive Cas9 expressing transposon library vector

    Plasmid

    #164138

    Purpose

    Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6, EF-1a

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Mutant ornithine transcarbamylase aa 30-114 deleted (OTC-delta)

    Plasmid

    #71878

    Purpose

    For mitochondrial unfolded protein response research

    Depositor

    Nicholas Hoogenraad

    Article

    Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

    Insert

    OTC-delta (Otc Rat)
    

    Use

    Tags

    Expression

    Mutation

    Deleted amino acid 30-114 from full length OTC

    Promoter

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115213

    Purpose

    Expresses RanCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    RanCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid

    #99277

    Purpose

    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance

    Depositor

    Jordan Miller

    Article

    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

    Inserts

    H2B-mOrange2-IRES-EGFP
    Puro
    

    Use

    Lentiviral

    Tags

    EGFP, H2B, IRES, PGK, and mOrange2

    Expression

    Mutation

    Promoter

    EF1 alpha-human and PGK-mouse

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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43. pET His6 TEV co-transformation cloning vector (13S-B)

    Plasmid

    #48324

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET His6 TEV co-transformation cloning vector (13K-B)

    Plasmid

    #48316

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pET StrepII TEV co-transformation cloning vector (13S-R)

    Plasmid

    #48328

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pET StrepII TEV co-transformation cloning vector (13K-R)

    Plasmid

    #48319

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCMV-Frankenbody-HA-StayGold-IRESv4-HaloTag-tdMCP-CAAX

    Plasmid

    #241145

    Purpose

    Expresses Anti-HA frankenbody-StayGold and HaloTag-tdMCP-CAAX to track mature and nascent SunTag-tagged proteins and track MS2-tagged mRNA anchored to the plasma membrane

    Depositor

    Tim Stasevich

    Article

    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

    Inserts

    Anti-HA frankenbody
    tdMCP - CAAX
    

    Use

    Tags

    (n2)oxStayGold(c4) E147D and HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-C1

    Plasmid

    #196877

    Purpose

    Neuron-specific expression of mOrange2 reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag

    Plasmid

    #132409

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dRanCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H147A; H1044A

    Promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pET His6 Thioredoxin TEV co-transformation cloning vector (13K-T)

    Plasmid

    #48322

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6 and Thioredoxin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge