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Showing: 4361 - 4400 of 8321 results

  1. pZC14 (pFA6a-PSc-6xGly-V5-HphMX)

    Plasmid
    #44079
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44079-plasmid-map-sequence-id-61697

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  2. pCB30

    Plasmid
    #111910
    Purpose
    Expresses gRNA to target URA3 knockout locus and has KanMX marker
    Depositor
    Yi Tang
    Insert
    gRNA cassette targeting URA3 knockout locus
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    SNR52p
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    111910-plasmid-map-sequence-id-218310

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  3. MYLK4

    Plasmid
    #39175
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MYLK4 (MYLK4 Human)
    Use
    Baculovirus expression
    Tags
    His Tev
    Expression
    Mutation
    N371S, codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. pHT-PRD

    Plasmid
    #87755
    Purpose
    Bacterial expression vector for producing 6x histidine tagged dynamin 1a proline rich domain (residues 746-864)
    Depositor
    Yu-Wen Hwang
    Insert
    Semi-synthetic gene coding for dynamin 1a proline rich domain (residues 746-864)
    Use
    Tags
    6X histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNA polymerase promoter
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87755-plasmid-map-sequence-id-173677

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  5. pLV-Enh eGFP Reporter-muKC2-X14

    Plasmid
    #59238
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Krt8-ex5-8
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pLV-Enh eGFP Reporter-muKC2-IGR12

    Plasmid
    #59281
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    1.5kb upstream krt5
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. RAC3

    Plasmid
    #38821
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RAC3 (rac3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. HDHD1A

    Plasmid
    #39039
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HDHD1A (PUDP Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    G102R
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. isoform PKC Gamma (JCE692)

    Plasmid
    #89780
    Purpose
    yeast expression of mouse PKC Gamma with yeast PKC1 promoter and ADH1 terminator
    Depositor
    Juan Carlos Igual
    Insert
    PKC Gamma (Prkcg Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    yeast PKC1 promoter (-600 to -1)
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    89780-plasmid-map-sequence-id-177898

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  10. His8:MBP-tev-Gly-eK-Flv

    Plasmid
    #83383
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83383-plasmid-map-sequence-id-173970

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  11. PTPRJ

    Plasmid
    #38889
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPRJ (PTPRJ Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    W1203A inactivating mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pBABEpuro-CRAF_S257W

    Plasmid
    #51126
    Purpose
    mammalian expression of wt CRAF S257W
    Depositor
    Matthew Meyerson
    Insert
    CRAF (RAF1 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    S257W
    Promoter
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51126-plasmid-map-sequence-id-77384

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  13. p14-9VN9-3A

    Plasmid
    #50932
    Purpose
    Expresses human SRP14 and SRP9-3A proteins as a fusion protein in mammalian cells.
    Depositor
    Katharina Strub
    Insert
    SRP14 and SRP9 (SRP14, SRP9 Human)
    Use
    Tags
    VN tag and flag tag
    Expression
    Mammalian
    Mutation
    Amino acids 60, 61 and 64 of SRP9 changed to alan…
    Promoter
    CMV
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pTF274 (pFA6a-PSc-6xGly-3xFlag-HphMX)

    Plasmid
    #44077
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44077-plasmid-map-sequence-id-61678

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  15. pZC12 (pFA6a-PSc-6xGly-V5-KanMX)

    Plasmid
    #44091
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44091-plasmid-map-sequence-id-61695

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  16. pUASp YFP Rholike WT

    Plasmid
    #52239
    Purpose
    Drosophila expression of YFP-Rholike
    Depositor
    Matthew Scott
    Insert
    Rholike (RhoL Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Promoter
    GAL4
    Available since
    April 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. PDLIM1

    Plasmid
    #38809
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PDLIM1 (PDLIM1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Truncated (see comments)
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pQdCas9-sggfp

    Plasmid
    #236184
    Purpose
    The plasmid pQdCas9-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the gfp gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    Quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
    Use
    Tags
    Expression
    Mutation
    Promoter
    Quorum sensing promoter
    Available since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    236184-plasmid-map-sequence-id-470109

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  21. pQdCas12a.sggfp(C)

    Plasmid
    #236042
    Purpose
    The plasmid pQdCas12a.sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236042-plasmid-map-sequence-id-469925

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  22. pET15bHis6-pG-Tnp

    Plasmid
    #124884
    Purpose
    To transform BL21-Gold(DE3) and induced by IPTG for His-proteinG-Tnp (hyperactive mutant) fusion protein.
    Depositor
    Keji Zhao
    Insert
    protein G's C1D1C2D2C3 domains contained in the protein G gBLOCK.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    124884-plasmid-map-sequence-id-243752

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  23. pcDNA3.1-hSyncytin-1(AAAR)

    Plasmid
    #182442
    Purpose
    hSyncytin-1, furin cleavage site RNKR>AAAR(314-317)
    Depositor
    Masaharu Somiya
    Insert
    ERVW-1 (ERVW-1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    furin cleacage site RNKR>>AAAR(314-317)
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    182442-plasmid-map-sequence-id-357724

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  24. pAG416-GAL-SUP35

    Plasmid
    #181705
    Purpose
    Galactose inducible expression of SUP35
    Depositor
    Alejandro Chavez
    Insert
    SUP35 (SUP35 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    181705-plasmid-map-sequence-id-466832

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  28. 0A-1169

    Plasmid
    #220935
    Purpose
    A QUAS line that expresses VSR proteins in Ae. aegypti when crossed with a driver line, Opie-2 dsRed.
    Depositor
    Omar Akbari
    Insert
    Cricket paralysis virus (CrPV) 1A protein, Nodamura virus (NoV) B2 protein, and flock house virus (FHV) B2
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    hsp70
    Available since
    March 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    220935-plasmid-map-sequence-id-440220

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  29. pET-Impact-T4gp32(L301G)

    Plasmid
    #228209
    Purpose
    Expression in BL21(DE3) of single strand DNA binding protein L301G
    Depositor
    Stephen Benkovic
    Insert
    61
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Mutated leucine 301 to glycine (L301G), improves …
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228209-plasmid-map-sequence-id-453946

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  30. 3xFlag-Rab34

    Plasmid
    #232458
    Purpose
    Expresses 3xFlag-tagged human Rab34 in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    Rab34 (RAB34 Human)
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232458-plasmid-map-sequence-id-463631

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  31. pMDC32_PbGH3

    Plasmid
    #232335
    Purpose
    Generation of overexpressing Arabidopsis thaliana and Brassica napus lines to characterize the role of PbGH3 in plant.
    Depositor
    Edel Pérez-López
    Insert
    PbGH3
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232335-plasmid-map-sequence-id-463036

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  37. pdCas9-tdTomato

    Plasmid
    #232631
    Purpose
    catalytically inactive dCas9 for localization; labeled with tdTomato
    Depositor
    Karsten Rippe
    Insert
    dCas9-tdTomato
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV (TATA box removed)
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232631-plasmid-map-sequence-id-462150

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  38. pT7RNAPNev-FSR22

    Plasmid
    #216338
    Purpose
    Arabinose-inducible PBad promoter driving expression of the evolved N-terminal portion (residues 1-179) of T7RNAP translationally fused to anti-SARS-CoV-2 RBD DARPin FSR22
    Depositor
    Mark Styczynski
    Insert
    T7RNAPN-FSR22 fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    216338-plasmid-map-sequence-id-456869

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  39. pCG-ACTB-C4

    Plasmid
    #229846
    Purpose
    Expresses wild-type Cas9 and gRNA for ACTB gene.
    Depositor
    Natsuko Chiba
    Insert
    guide RNA for ACTB gene
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    229846-plasmid-map-sequence-id-457514

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  40. pCG-NT2

    Plasmid
    #229848
    Purpose
    Expresses wild-type Cas9 and gRNA with non-target sequence.
    Depositor
    Natsuko Chiba
    Insert
    guide RNA with non-target sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    229848-plasmid-map-sequence-id-457513

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Showing: 4361 - 4400 of 8321 results