We narrowed to 10,942 results for: NSI;

1. pMTTH+mEGFP-LANA

   Plasmid

   #157801

   Purpose

   Expression of mEGFP and LANA peptide fusion protein

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   mEGFP-LANA
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTAC

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMN-018

   Plasmid

   #195422

   Purpose

   AAVS1 Integratable mCherry Reporter for synZiFTR ZF10

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   4X ZF10 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MAC_N_PSA1

   Plasmid

   #111690

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   PSA1 (PSMA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a_Pro-IL-36α

   Plasmid

   #214343

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL36A (IL36A Human)
   

   Use

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRK5-myc-PAT1

   Plasmid

   #88856

   Purpose

   myc-TAG overexpression

   Depositor

   David Sabatini

   Article

   Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

   Insert

   SLC36A1 (SLC36A1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. WNV NS2B-NS3 protease (based on PDB 2YOL, catalytically active); aliases: West Nile Virus NS2B-NS3 fusion protein

   Plasmid

   #204798

   Purpose

   Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   West Nile NS2B-NS3 fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pSW609 - N001

   Plasmid

   #115993

   Purpose

   intermediate vector for GreenGate cloning method, contains modules from A-F

   Depositor

   Sebastien Wolf

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   H-A adapter:pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
   

   Use

   Unspecified

   Tags

   HDEL and SP(ER)

   Expression

   Mutation

   Promoter

   Available since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAB082b

   Plasmid

   #79234

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ-Cry1Ac-d3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppro1

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBS AluYa5rescueA70D

   Plasmid

   #78657

   Purpose

   Generates Alu inserts suitable for rescue

   Depositor

   Astrid Roy-Engel

   Article

   Wagstaff et al PLoS Genet. 2012;8(8):e1002842. doi: 10.

   Insert

   Ya5rescue cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-S9

    Plasmid

    #132948

    Purpose

    Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S9 (rpsI E.coli)
    

    Use

    Tags

    N-terminal 6xHis-tag

    Expression

    Bacterial

    Mutation

    E52C

    Promoter

    T7

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMRX-IP-GFP-LC3-mRuby3

    Plasmid

    #184898

    Purpose

    Stable expression of GFP-LC3-mRuby3 in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Okawa et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    LC3B
    

    Use

    Tags

    EGFP and mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMZ-440

    Plasmid

    #195464

    Purpose

    Lentiviral Integratable, 4OHT/TMX- and GZV-controllable expression of synZiFTR: KRAB-ZF3

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pSFFV - KRAB - ZF3 - ERT2 - SMASh
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRK5-mFzd3-1D4

    Plasmid

    #42265

    Depositor

    Jeremy Nathans, Chris Garcia

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 3 (Fzd3 Mouse)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE)

    Plasmid

    #140226

    Purpose

    Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and zeocin resistance

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    Luciferase + crIFNg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAIDFA-CMV-NmScarlet-mAID

    Plasmid

    #140616

    Purpose

    Expression of OsTIR1F74A and mScarlet-mAID protein in DT40 cells under the control of CMV promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1 mutated F74A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGAL4-DBD-MCS-long

    Plasmid

    #145246

    Purpose

    Backbone vector expressing GAL4 DNA-binding domain followed by MCS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p426 25Q GPD

    Plasmid

    #1181

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 25Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-Flag-YAP-S94/F95A

    Plasmid

    #33057

    Depositor

    Kunliang Guan

    Article

    Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S94A, F95A

    Promoter

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pcihygroflagsynhDAT

    Plasmid

    #19990

    Depositor

    Jonathan Javitch

    Article

    Saunders et al Proc Natl Acad Sci U S A. 2000 Jun 6. 97

    Insert

    Dopamine transporter (SLC6A3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAB082h

    Plasmid

    #79240

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Ppro1 rpoZ-Cry1Ac-d23C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ppro1

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p426 103Q GAL

    Plasmid

    #1188

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 103Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

22. pENTR-mOvol1

    Plasmid

    #120359

    Purpose

    Gateway entry vector containing mouse Ovol1.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    Ovol1 (Ovol1 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on and CBh

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MAC_C_SFXN1

    Plasmid

    #111676

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    SFXN1 (SFXN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Lsg-LMNA-1 + 2

    Plasmid

    #199278

    Purpose

    nicking sgRNA to induce LMNA (c.1824C > T) mutation using PE3

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    LMNA nick
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGL3-VEGFR2-570

    Plasmid

    #21309

    Depositor

    Donald Ingber

    Article

    Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

    Insert

    VEGFR2 promoter (KDR Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

26. pSLQ8702 pHR-TRE3G-AcrVA1-EF1a-sfGFP-T2A-rtta-WPRE

    Plasmid

    #140229

    Purpose

    Dox-inducible expression of anti-Cpf1 AcrVA1. Constitutive EF1a driven rtTA and GFP

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    AntiCRISPR protein AcrVA1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pZH520

    Plasmid

    #102673

    Purpose

    Tetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMTTH+eGFP-bromo5

    Plasmid

    #157804

    Purpose

    Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    eGFP-bromo5
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAB061i2

    Plasmid

    #79229

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Ppro1 rpoZ-HA4 (Y87A); PlacIq lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ppro1; PlacIq

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pWPXL-NP1

    Plasmid

    #72654

    Purpose

    Constitutive expression of NP1

    Depositor

    Ramon Trullas

    Article

    Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

    Insert

    NP1 (Nptx1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Zyxin-GFP

    Plasmid

    #205095

    Purpose

    Express GFP-tagged human zyxin in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    zyxin (ZYX Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pZH521

    Plasmid

    #102674

    Purpose

    Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRK5-mFzd7-1D4

    Plasmid

    #42269

    Depositor

    Jeremy Nathans, Chris Garcia

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 7 (Fzd7 Mouse)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

34. pMVS-V1

    Plasmid

    #183094

    Purpose

    Basic conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus

    Depositor

    Lars Angenent, Bastian Molitor

    Article

    Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

    Inserts

    pME2001
    thermostable neomycin phosphotransferase gene
    

    Use

    Bacterial propagation and conjugative

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTRE2-Hyg-omMx1

    Plasmid

    #52420

    Purpose

    Inducible expression (Tet-Off system) of the rainbow trout antiviral Mx1 protein

    Depositor

    Bertrand Collet

    Article

    Lester et al J Virol Methods. 2012 Jun;182(1-2):1-8.

    Insert

    Oncorhynchus mykiss interferon-induced GTP-binding protein Mx1 (mx1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE2

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pZH517

    Plasmid

    #102670

    Purpose

    Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pPnos (GB0072)

    Plasmid

    #68166

    Purpose

    Provides the Agrobacterium tumefaciens Nopaline synthase promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Nos promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAB064d

    Plasmid

    #79197

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Plac62 luxAB; Ppro1 434cI-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac62; Ppro1

    Available since

    Dec. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLPCX-PECAMTL

    Plasmid

    #45851

    Depositor

    Martin Schwartz

    Article

    Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

    Insert

    PECAM1 (PECAM1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p426 25Q GAL

    Plasmid

    #1187

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 25Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only