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pcDNA3.1 puro Nodamura B2

Plasmid

#17228

Depositor

Christopher Sullivan

Publication

Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

Insert

Nodamura B2 (NoVsRNA1gp2 Nodamura Virus)

Expression

Mammalian

Mutation

2 silent point mutations

Available since

June 22, 2011

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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GFP-TREX1

Plasmid

#27219

Depositor

Judy Lieberman

Publication

Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

Insert

TREX1 (TREX1 Human)

Tags

GFP

Expression

Mammalian

Mutation

n/a

Available since

Jan. 26, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLVU/RED

Plasmid

#24178

Depositor

Lars Ittner

Publication

Krupka et al Plasmid. 2010 May;63(3):155-60. doi: 10.

Insert

Chloramphenicol resistance gene (CmR) - ccdB gene

Use

Gateway Cloning and Lentiviral

Tags

DsRED

Expression

Mammalian

Available since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pET28a-LIC

Plasmid

#26094

Purpose

SGC Empty backbone for bacterial expression

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Tags

6xHis and Thrombin cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Aug. 20, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

9 Articles

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pDONR223-EGFR

Plasmid

#23935

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EGFR (EGFR Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pCHERRY3

Plasmid

#24659

Depositor

Tanya Parish

Publication

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

mCherry

Expression

Bacterial

Available since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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p3962 pcDNA3-IRF3-FL

Plasmid

#22860

Depositor

Peter Howley

Publication

Kirshner et al J Virol. 2005 Jul . 79(14):9320-4.

Insert

IRF3 (IRF3 Human)

Expression

Mammalian

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTriEx-mCherry-PA-Rac1-T17N

Plasmid

#22029

Purpose

Expression of photoactivatable Rac1 (dominant-negative mutant).

Depositor

Klaus Hahn

Publication

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…

Promoter

CMV, p10

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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FLAG-Ago2

Plasmid

#21538

Depositor

Edward Chan

Publication

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Tags

3XFLAG

Expression

Mammalian

Mutation

The first 7aa of this Ago2 protein are different …

Available since

Sept. 21, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pDsRed2-Keap1

Plasmid

#21551

Depositor

Yue Xiong

Publication

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

kelch-like ECH-associated protein 1 (KEAP1 Human)

Expression

Mammalian

Available since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MSCV JMJD3 mutant

Plasmid

#21214

Depositor

Paul Khavari

Publication

Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

Insert

JMJD3 (KDM6B Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

H1390A

Available since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA3-HA2-DDB1

Plasmid

#19909

Depositor

Yue Xiong

Publication

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

Damage-specific DNA binding protein 1 (DDB1 Human)

Tags

HA

Expression

Mammalian

Mutation

please see depositor comment below

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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-3.6kb human IFN-gamma luc

Plasmid

#17599

Depositor

Howard Young

Publication

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

IFN gamma promoter (IFNG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

-3.6 kb to +70 of promoter

Available since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLOVE-GFP

Plasmid

#15949

Depositor

Miguel Ramalho-Santos

Publication

Blelloch et al Cell Stem Cell. 2007 Sep 13;1(3):245-7.

Insert

Green Fluorescent Protein

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

The recombination reaction required to clone &quo…

Available since

Nov. 7, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCDNA3-Flag MKK6

Plasmid

#13517

Depositor

Roger Davis

Publication

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK6 (MAP2K6 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pRK7-FLAG-TSC1

Plasmid

#8995

Depositor

John Blenis

Publication

Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Insert

TSC1 (TSC1 Human)

Tags

FLAG

Expression

Mammalian

Available since

Nov. 11, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hSyn-FLEX-TeLC-P2A-dTomato

Plasmid

#159102

Purpose

Cre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.

Depositor

Sandeep Datta

Publication

Pashkovski et al Nature. 2020 Jul;583(7815):253-258. doi:

Insert

TeLC-P2A-NLS-dTomato

Use

AAV

Promoter

hSynapsin

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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AAV.CBA.eGFP.2A.P301L-Tau

Plasmid

#140425

Purpose

AAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTau

Depositor

Bradley Hyman

Publication

Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

Insert

eGFP-2A-Tau(P301L) (MAPT Synthetic, Human)

Use

AAV

Tags

eGFP

Mutation

changed Proline 301 to Leucine

Promoter

CBA

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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LentiGuide-Neo

Plasmid

#139449

Purpose

Lentiviral vector with gRNA scaffold and neomycin selectable marker

Depositor

Caroline Goujon

Publication

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

no sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro

Plasmid

#141374

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp8 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

10 Articles

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pH2B-emiRFP670

Plasmid

#136571

Purpose

Histone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Publication

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

H2B-emiRFP670

Tags

emiRFP670

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCRZeroT

Plasmid

#120276

Purpose

Bifunctional vector for TA-cloning and blunt-end cloning of PCR-products using ZERO-backgound system

Depositor

Ken Motohashi

Publication

Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

Type

Empty backbone

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMBP-AsCas12a

Plasmid

#113430

Purpose

Expresses 10xHis-MBP fused to AsCas12a

Depositor

Jennifer Doudna

Publication

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AsCas12a

Tags

MBP

Expression

Bacterial

Promoter

T7

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pmGFP-P2A-K(AAA)20-P2A-RFP

Plasmid

#105688

Purpose

Double fluorescent stalling reporter K(AAA)20

Depositor

Ramanujan Hegde

Publication

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K(AAA)20
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pAAV-M13-TEV-C-P2A-TdTomato

Plasmid

#92391

Purpose

produces AAV expressing Cal-light M13-TevC component and TdTomato

Depositor

Hyungbae Kwon

Publication

Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

M13-TEV-C-P2A-TdTomato

Use

AAV and Synthetic Biology

Tags

TdTomato

Expression

Mammalian

Promoter

human synapsin

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pTetON-EGFP

Plasmid

#89453

Purpose

Tet/Dox-inducible expression of EGFP in mammalian cells (control)

Depositor

Brad Zuchero

Publication

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

EGFP

Expression

Mammalian

Promoter

TRE3G

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMCB320

Plasmid

#89359

Purpose

3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

Depositor

Michael Bassik

Publication

Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

Insert

puromycin resistance, mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

33 Articles

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mCherry-DFCP1

Plasmid

#86746

Purpose

N-terminal mCherry-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Publication

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Insert

human DFCP1 (ZFYVE1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pTriEx-mCherry-LOV2

Plasmid

#81041

Purpose

Mammalian or insect expression for wt LOV2

Depositor

Klaus Hahn

Publication

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV, p10

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pET30a-AlkB

Plasmid

#79050

Purpose

Express wild type AlkB protein in E. coli BL21(DE3)

Depositor

Tao Pan

Publication

Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

Insert

AlkB (alkB E.coli)

Tags

His tag

Expression

Bacterial

Mutation

A truncated AlkB with deletion of the amino (N)-t…

Promoter

T7

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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