163,606 results

1. pCT5-bac1.8

   Plasmid

   #119871

   Purpose

   Constitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

   Depositor

   Claudia Schmidt-dannert

   Article

   Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

   Inserts

   CymR'
   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   SNP in 592 bp

   Promoter

   PserA promoter and Pveg promoter with CuO sequence

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmiRFP720-N1

   Plasmid

   #136560

   Purpose

   A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   miRFP720
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2 Notch1 Full Length-6MT

   Plasmid

   #41728

   Depositor

   Raphael Kopan

   Article

   Schroeter et al Nature. 1998 May 28;393(6683):382-6.

   Insert

   Notch-1 Full Length (Notch1 Mouse)
   

   Use

   Tags

   6xMyc

   Expression

   Mammalian

   Mutation

   L1997V; Contains murine Notch-1 protein up to ami…

   Promoter

   Available Since

   Dec. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR_Gal4UAS_Blinatumomab_PGK_mCherry

   Plasmid

   #85437

   Purpose

   [Edit] Lentiviral vector for Gal4 inducible Blinatumomab and constitutive mCherry expression

   Depositor

   Wendell Lim

   Article

   Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

   Insert

   Blinatumomab
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-shRNA-ctrl

   Plasmid

   #85741

   Purpose

   shRNA ctrl

   Depositor

   Hongjun Song

   Article

   Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

   Insert

   shRNA ctrl
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   Available Since

   April 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pH2B-emiRFP670

   Plasmid

   #136571

   Purpose

   Histone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   H2B-emiRFP670
   

   Use

   Tags

   emiRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV6-Neo-ISG15-LRAA

   Plasmid

   #80405

   Purpose

   overexpression of nonconjugative ISG15

   Depositor

   Isidoro Martinez

   Article

   Gonzalez-Sanz et al J Virol. 2016 Jan 13;90(7):3428-38. doi:

   Insert

   ISG15 (ISG15 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   nonconjugative ISG15, LRAA

   Promoter

   CMV

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME pHluorin2

   Plasmid

   #73794

   Purpose

   ratiometric pHluorin2 sequence in a middle entry clone

   Depositor

   David Raible

   Article

   Stawicki et al Dis Model Mech. 2014 Jul;7(7):847-56. do

   Insert

   ratiometric pHluorin2
   

   Use

   Middle entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-miRFP720

   Plasmid

   #197213

   Purpose

   Expresses the protein of miRFP720 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   miRFP720
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. YAP/TAZ-TRE-mStrawberry reporter

    Plasmid

    #158682

    Purpose

    This plasmid is a YAP/TAZ pathway reporter. It has a YAP/TAZ-responsive synthetic promoter driving the expression of mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-PD-1 (Y223F, Y248F)-mGFP

    Plasmid

    #180790

    Purpose

    Expression of human PD-1 (Y223F, Y248F) fused to mEGFP

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    PD-1 (Y223F, Y248F) (PDCD1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    PD-1 (Y223F, Y248F)

    Promoter

    SFFV

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pscAAV-CAG-GFP

    Plasmid

    #83279

    Purpose

    recombinant AAV vector packaging self-complementary GFP under the CAG promoter

    Depositor

    Mark Kay

    Article

    Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

    Insert

    enhanced green fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. CFTR-HiBiT Fusion Vector

    Plasmid

    #236890

    Purpose

    Express CFTR WT HiBiT in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    CFTR (CFTR Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pHBS1503 [IBB-GFP-mCherry3E]-[BFP-TDP43 WT]

    Plasmid

    #133327

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAMD1-4

    Plasmid

    #61565

    Purpose

    Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

    Type

    Empty backbone

    Use

    Synthetic Biology; Mini-tn5 vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHBS1501 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaRRM]

    Plasmid

    #133325

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of CTD in full-length TDP43

    Promoter

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPGK-SB13

    Plasmid

    #236078

    Purpose

    SB13 transposase, for use with Sleeping Beauty transponson plasmids

    Depositor

    Masashi Narita

    Article

    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

    Insert

    SB13 transposase
    

    Use

    Sleeping beauty (sb) transposase plasmid

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-GfaABC1D-Cre-4x6T

    Plasmid

    #196410

    Purpose

    Astrocytic expression of Cre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Has Service

    AAV5

    Insert

    Cre
    

    Use

    AAV; Astrocyte-selective

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJYS1Peftu

    Plasmid

    #85546

    Purpose

    Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    recT
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    etfu and unknown

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. v5 Cas9-eVLP

    Plasmid

    #228468

    Purpose

    Expresses MMLVgag(C507V)–Cas9 for producing v5 Cas9-eVLPs

    Depositor

    David Liu

    Article

    Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

    Insert

    MMLVgag(C507V)–Cas9
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    MMLVgag(C507V)

    Promoter

    CMV

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pWZL-Blast FAPwt

    Plasmid

    #210643

    Purpose

    Expresses the FAP (Fibroblast Activation Protein) protein.

    Depositor

    Jing Yang

    Article

    Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

    Insert

    Fibroblast Activation Protein Alpha (FAP Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    none

    Promoter

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. MCS-13X Linker-BioID2-HA

    Plasmid

    #80899

    Purpose

    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.

    Depositor

    Kyle Roux

    Article

    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    HA and humanized A. aeolicus BioID2 with R40G

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBAD-GINKO2

    Plasmid

    #177116

    Purpose

    Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO2

    Depositor

    Robert Campbell

    Article

    Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

    Insert

    GINKO2
    

    Use

    Tags

    6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLJC6-3XHA-TMEM192

    Plasmid

    #104434

    Purpose

    Expresses 3XHA-TMEM192 in mammalian cells

    Depositor

    David Sabatini

    Article

    Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pHR-CD28-mCherry

    Plasmid

    #223593

    Purpose

    Lentiviral expression of human CD28 with mCherry tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET29a-human histone 4-in-One

    Plasmid

    #223237

    Purpose

    Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

    Depositor

    Weixing Zhao

    Inserts

    His6-VSVG-Histone H2A
    Histone H2B
    Histone H3
    Histone H4-TEV-His6-Dual StrepII
    

    Use

    Tags

    Dual StrepII, His6, TEV protease site, and VSVG

    Expression

    Bacterial

    Mutation

    A103G

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. OpenCRISPR-1 sgRNA-12nt stem

    Plasmid

    #221567

    Purpose

    Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

    Depositor

    Ali Madani

    Article

    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

    Insert

    OpenCRISPR-1 sgRNA 12nt stem loop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pcDNA3.1-2xFLAG-SREBP-2

    Plasmid

    #26807

    Depositor

    Timothy Osborne

    Article

    Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

    Insert

    Sterol Regulatory Element Binding Protein 2 (SREBF2 Human)
    

    Use

    Tags

    2x FLAG

    Expression

    Mammalian

    Mutation

    L7V, G76S, K395R and I482L

    Promoter

    CMV

    Available Since

    July 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET15b-RvLEAMshort

    Plasmid

    #218122

    Purpose

    Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

    Depositor

    Ci Ji Lim

    Article

    Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

    Insert

    RvLEAMshort
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Truncated form (58-181aa)

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPSU1

    Plasmid

    #89439

    Purpose

    High copy number plasmid 1 to prepare Penn State DNA molecular weight ladders

    Depositor

    Song Tan

    Article

    Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.

    Inserts

    500 bp EcoRV fragment
    1000 bp EcoRV fragment
    1500 bp EcoRV fragment
    2000 bp EcoRV fragment
    500 bp PstI fragment
    700 bp PstI fragment
    800 bp PstI fragment (EHMT2 Human)
    900 bp PstI fragment
    1000 bp PstI fragment (BMI1 Human)
    2000 bp PstI fragment
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge