165,214 results

1. pLenti-PGK-Venus-Fluc (puro)

   Plasmid

   #140328

   Purpose

   Lentivirus for expression of Venus-Fluc (firefly luciferase) fusion protein

   Depositor

   Roland Friedel

   Article

   Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

   Insert

   Venus-Firefly Luciferase (luc2)
   

   Use

   Lentiviral

   Promoter

   human PGK promoter

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX4T-E2-25K

   Plasmid

   #63572

   Purpose

   Expression of human Ubiquitin conjugating enzyme E2-25K

   Depositor

   Titia Sixma

   Article

   Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

   Insert

   UBE2-25K (UBA7 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Isoform 1 (200 resiudes)

   Promoter

   tac

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCE048-SiT-Cas12a

   Plasmid

   #128124

   Purpose

   Express both AsCas12a and CRISPR arrays on single transcripts

   Depositor

   Randall Platt

   Article

   Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

   Insert

   AsCas12a-Triplex
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-Tubulin-C-18

   Plasmid

   #55148

   Purpose

   Localization: Microtubules, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

   Insert

   Tubulin (TUBA1B Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-mCherry

   Plasmid

   #114472

   Purpose

   mCherry Control

   Depositor

   Karl Deisseroth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   mCherry
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR_Gal4UAS_humanIL12flexi _IRES_mCherry_PGK_tBFP

   Plasmid

   #85428

   Purpose

   Lentiviral vector for Gal4 inducible humanIL-12flexi with mCherry reporter and constitutive tBFP expression

   Depositor

   Wendell Lim

   Article

   Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

   Insert

   human IL-12
   

   Use

   Lentiviral

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-15b-EK_C122S_His5

   Plasmid

   #49048

   Purpose

   Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

   Depositor

   Hans Brandstetter

   Article

   Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

   Insert

   Enteropeptidase, peptidase domain (TMPRSS15 Bovine)
   

   Tags

   5xHis

   Expression

   Bacterial

   Mutation

   P92R, E185D, C122S

   Promoter

   T7

   Available Since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-HDAC2-H141A

   Plasmid

   #115346

   Purpose

   Expresses mouse HDAC2 cDNA with H141A point mutation

   Depositor

   Javier Gonzalez-Maeso

   Article

   Kurita et al Nat Neurosci. 2012 Sep;15(9):1245-54. do

   Insert

   Histone deacetylase 2 (Hdac2 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H141A

   Promoter

   CMV

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. CROPseq-multi-v2-Puro-BsmBI_entry (pRW1484)

   Plasmid

   #225752

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid

    #139093

    Purpose

    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    dxCas9-NG-VPR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-HA-USP39

    Plasmid

    #22581

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP39 (USP39 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA-HDAC6.DC-FLAG

    Plasmid

    #30483

    Depositor

    Tso-Pang Yao

    Article

    Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

    Insert

    HDAC6 (HDAC6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Catalytically inactive mutant (H216/611A)

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC18T-mini-Tn7T-Gm

    Plasmid

    #63121

    Purpose

    mini-Tn7 vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 17, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCAG-G-Flamp2-mut

    Plasmid

    #192783

    Purpose

    control probe of G-Flamp2, insensitive to cAMP

    Depositor

    Jun Chu

    Article

    Liu et al Front Pharmacol. 2022 May 12;13:902290.

    Insert

    G-Flamp2-mut
    

    Tags

    His tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-GRAB_eCB2.0

    Plasmid

    #164604

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Mutation

    S383T

    Promoter

    hSyn

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLG009 (DRT type 1)

    Plasmid

    #157887

    Purpose

    Expresses the DRT type 1 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    DRT type 1 (RT-nitrilase)
    

    Expression

    Bacterial

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-PGK-Neo-PIP-FUCCI

    Plasmid

    #118616

    Purpose

    Lentiviral expression of the cell cycle marker PIP-FUCCI

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-FUCCI
    

    Use

    Lentiviral

    Tags

    mCherry and mVenus

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSBbi_RP_MicroID2-HA

    Plasmid

    #188521

    Purpose

    Encodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cells

    Depositor

    James Londino

    Article

    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

    Insert

    MicroID2
    

    Use

    Transposon mediated gene integration

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. CPSF1

    Plasmid

    #155475

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CPSF1 (CPSF1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-rtTA

    Plasmid

    #175274

    Purpose

    AAV vector mediating neuron-specific expression of rtTA

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    Reverse tetracycline-controlled transactivator (rtTA)
    

    Use

    AAV

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAKTaq

    Plasmid

    #25712

    Purpose

    Bacterial expression of Taq polymerase

    Depositor

    David Engelke

    Article

    Engelke et al Anal Biochem. 1990 Dec . 191(2):396-400.

    Insert

    Taq polymerase
    

    Expression

    Bacterial

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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22. p426-SNR52p-gRNA.CAN1.Y-SUP4t

    Plasmid

    #43803

    Purpose

    Encodes a gRNA that targets Can1.Y in yeast.

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    CAN1.y gRNA (CAN1 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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23. shCD44-2 pRRL

    Plasmid

    #19123

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    small hairpin RNA against CD44 (CD44 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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24. pEGFP(C3)-Nup153

    Plasmid

    #64268

    Purpose

    GFP-tagged mammalian expression of Nup153

    Depositor

    Birthe Fahrenkrog

    Article

    Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

    Insert

    NuP153 (NUP153 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pmCherry-C1-Nir1-LNS2

    Plasmid

    #220084

    Purpose

    high affinity PA biosensor with red fluorophore (also known as mCherry-PILS-Nir1)

    Depositor

    Gerry Hammond

    Article

    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

    Insert

    PITPNM3 (PITPNM3 Human)
    

    Tags

    mCherry-GGSGGM

    Expression

    Mammalian

    Mutation

    amino acids 613-897

    Promoter

    CMV

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAC-BETAipi

    Plasmid

    #53277

    Purpose

    Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

    Insert

    idi
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    endogenous promoter

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. Flag-HA-USP38

    Plasmid

    #22580

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP38 (USP38 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFLAG_WT MDA5

    Plasmid

    #216798

    Purpose

    Expresses wild type (wt) melanoma differentiation-associated protein 5

    Depositor

    Sun Hur

    Insert

    melanoma differentiation-associated protein 5 (IFIH1 Human)
    

    Expression

    Mammalian

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLG011 (DRT type 3)

    Plasmid

    #157889

    Purpose

    Expresses the DRT type 3 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    DRT type 3 (RT + RT)
    

    Expression

    Bacterial

    Available Since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pscAAV-CAG-GFP

    Plasmid

    #83279

    Purpose

    recombinant AAV vector packaging self-complementary GFP under the CAG promoter

    Depositor

    Mark Kay

    Article

    Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

    Insert

    enhanced green fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. S1m tagged DANCR

    Plasmid

    #138585

    Purpose

    For in vivo tagging

    Depositor

    Yin-Yuan Mo

    Article

    Hu et al Cell Death Differ. 2019 Dec 5. pii: 10.1

    Insert

    DANCR (DANCR Human)
    

    Use

    Lentiviral

    Tags

    S1m tag

    Promoter

    MSCV

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSpCas9n(BB)-2A-Puro (PX462) V2.0

    Plasmid

    #62987

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEGFP-C2_SEPT9 i4

    Plasmid

    #71617

    Purpose

    mammalian expression of human SEPT9 i4 with a GFP fusion

    Depositor

    Cristina Montagna, Elias Spiliotis

    Article

    Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

    Insert

    Septin 9 (SEPTIN9 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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34. AP1

    Plasmid

    #179678

    Purpose

    Accessory plasmids

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    [SD8] gIII
    

    Expression

    Bacterial

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. emiRFP670-cGAS

    Plasmid

    #231896

    Purpose

    Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    cGAS (CGAS Human)
    

    Tags

    emiRFP670

    Expression

    Mammalian

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV2/rh10

    Plasmid

    #112866

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes

    Depositor

    James M. Wilson

    Insert

    Rep2/Cap-rh10
    

    Use

    AAV

    Promoter

    Truncated P5

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMF230

    Plasmid

    #62546

    Purpose

    Broad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.

    Depositor

    Michael Franklin

    Article

    Nivens et al J Bacteriol. 2001 Feb;183(3):1047-57.

    Insert

    GFP mut2
    

    Use

    Broad host range plasmid

    Promoter

    trc

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBS_nanorod_105b

    Plasmid

    #120347

    Purpose

    phagemid containing all components for assembly of a DNA origami nanorod (10-helix bundle)

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

    Insert

    Staple sequences for a DNA origami nanorod
    

    Expression

    Bacterial

    Promoter

    none

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDESTpK7WG2mito

    Plasmid

    #158726

    Purpose

    Plant mitoTALEN vector assembly, Destination vector

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    mitochondrial presequence in front of attR1 in pk7WG2
    

    Expression

    Plant

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLG005 (Retron-TIR)

    Plasmid

    #157883

    Purpose

    Expresses the retron-TIR defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    Retron-TIR
    

    Expression

    Bacterial

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge