Skip to main content
Addgene
Showing: 5501 - 5550 of 12446 results

  3. mEmerald-Gamma-Actin2-N-18

    Plasmid
    #54104
    Purpose
    Localization: Actin , Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Gamma-Actin2
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. Myog'-2A-mCherryNLS-PGK-Puro

    Plasmid
    #69548
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm
    Myogenin 3' homology arm (Myog Mouse)
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Expression
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69548-plasmid-map-sequence-id-122974

    Enlarge

  23. pHAGE2-TetO-ePOUSKM

    Plasmid
    #206398
    Purpose
    Expresses four genes (human ePOU, SOX2, KLF4, c-MYC) in mammalian cells, for lentivirus generation.
    Depositor
    Ralf Jauch
    Insert
    ePOU, SOX2, KLF4, c-MYC (KLF4, MYC, SOX2 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    206398-plasmid-map-sequence-id-409991

    Enlarge

  30. Myopathy Loop Mutant Nuclear Myosin I YFP

    Plasmid
    #60243
    Purpose
    Expresses a myopathy loop mutant (6 mutations) of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding activity
    Depositor
    Primal de Lanerolle
    Article
    Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.
    Insert
    Nuclear Myosin 1 (Myo1c Mouse)
    Use
    Tags
    EYFP and NLS
    Expression
    Mammalian
    Mutation
    Deleted I339, I340, G343, E344, E345,L346
    Promoter
    CMV
    Available since
    Dec. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  32. pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G

    Plasmid
    #70075
    Purpose
    2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 (RRM mutant) in mammalian cells
    Depositor
    Thomas Tuschl
    Article
    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d
    Insert
    Dnd1 (Dnd1 Mouse)
    Use
    Lentiviral
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    Y102A. Silent mutations are introduced to escape …
    Promoter
    Tet responsible promoter
    Available since
    Nov. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    70075-plasmid-map-sequence-id-129458

    Enlarge

  36. mEos2-NEEP21-N-18

    Plasmid
    #57407
    Purpose
    Localization: Endosomes, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    NEEP21
    Use
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    D114G
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57407-plasmid-map-sequence-id-213181

    Enlarge

  38. pX335-NQL002-WAPL-sgRNA1

    Plasmid
    #175550
    Purpose
    For transient expression of spCas9-nickase and one sgRNA targeting the mouse WAPL locus. Use together with pX335-NQL003-WAPL-sgRNA2 targeting construct.
    Depositor
    Elzo de Wit
    Article
    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:
    Insert
    spCas9-nickase and sgRNA against mouse WAPL STOP Codon
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    175550-plasmid-map-sequence-id-345078

    Enlarge

  39. pX335-NQL003-WAPL-sgRNA2

    Plasmid
    #175551
    Purpose
    For transient expression of spCas9-nickase and one sgRNA targeting the mouse WAPL locus. Use together with pX335-NQL002-WAPL-sgRNA1 targeting construct.
    Depositor
    Elzo de Wit
    Article
    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:
    Insert
    spCas9-nickase and sgRNA against mouse WAPL STOP Codon
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    175551-plasmid-map-sequence-id-345081

    Enlarge

Showing: 5501 - 5550 of 12446 results