We narrowed to 12,598 results for: 112

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Ec2Avs2

Plasmid

#188790

Purpose

Avs2 from Escherichia coli NCTC10650

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Ec2Avs2

Expression

Bacterial

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3_Chrm3_Csx30_Cre

Plasmid

#197603

Purpose

Cre reporter for mammalian cells with Chrm3 membrane anchor

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

Chrm3_Csx30_Cre

Expression

Mammalian

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a-trAtCCD8(pYL735)

Plasmid

#178286

Purpose

Expresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

trAtCCD8

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+6)

Plasmid

#199160

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+6)

Tags

MGHHHHHHGGA

Mutation

E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Rap1b

Plasmid

#118319

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

Rap1b (RAP1B Bovine)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pJMP12089

Plasmid

#222357

Purpose

Tn7 integrative vector with PabstBR promoter expression cassette and hygR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Type

Empty backbone

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_N_FLAG_UBA6

Plasmid

#197971

Purpose

Mammalian cell expression of FLAG-UBA6

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

UBA6 (UBA6 Human)

Tags

FLAG

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pYC1876

Plasmid

#158722

Purpose

Helper plasmid pYC1640 for C-terminal tagging with degradation tag ssrA

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

ssrA

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->myc-mtC11L34

Plasmid

#162226

Purpose

Lentiviral vector for the expression of Myc mtC11L34 in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

anti-PNE scFv C11L34

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3GS-->GssGFP-P2A-NLSIFP

Plasmid

#162235

Purpose

Lentiviral vector for the expression of TRE3GS-->GssGFP-P2A-NLSIFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

GFP - IFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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Mis12-targted Aurora B FRET sensor

Plasmid

#45231

Depositor

Michael Lampson

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12 targeted Aurora B FRET sensor

Expression

Mammalian

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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LV-H2B-mRFP1

Plasmid

#47376

Purpose

This was used as a nuclear marker

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

H2B

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Connexin43-GFP-APEX

Plasmid

#44440

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

Insert

Connexin43 -EGFP - pea APEX

Expression

Mammalian

Mutation

K14D, W41F, E112K mutations on APEX

Promoter

CMV

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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gRNA-POLQ-Halo

Plasmid

#211636

Purpose

sgRNA against POLQ

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA POLQ

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSEVA43-GG

Plasmid

#208014

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Promoter

14G

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2121

Plasmid

#190343

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (empty vector control)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Type

Empty backbone

Expression

Plant

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-Ptol

Plasmid

#218369

Purpose

Empty backbone for Tn7-site insertion with XylS-Pm expression system

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMOD3-proD/mClover/nat

Plasmid

#120330

Purpose

Bacterial mClover labeling

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mClover

Tags

D103N (see comment below)

Expression

Bacterial

Promoter

(None)

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGL001

Plasmid

#115674

Purpose

Fluorescent reporter for E.coli K12 fliC promoter activity

Depositor

Gene-Wei Li

Article

Parker et al J Bacteriol. 2019 Sep 6;201(19). pii: JB

Insert

fliC promoter fused with GFP

Tags

Green fluorescent protein (GFP)

Expression

Bacterial

Promoter

fliC promoter

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMG13

Plasmid

#122932

Purpose

Expression of the regulator tTA.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

tTA

Expression

Yeast

Promoter

Pcln3

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pFF145

Plasmid

#61446

Purpose

aTc-inducible Beta recombinase

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

Beta recombinase

Use

Synthetic Biology

Promoter

pTetO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pJMP12068

Plasmid

#222356

Purpose

Tn7 integrative vector with PabstBR promoter expression cassette and kanR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Type

Empty backbone

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-ClfB_N2N3-HIS-ybbr

Plasmid

#101717

Purpose

ClfB N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

Depositor

Hermann Gaub

Article

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

ClfB_N2N3

Tags

H3V 3C, HIS, and ybbr

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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ClhN-4V5-APEX2-URA

Plasmid

#207056

Purpose

For ORF tagging with 4V5-APEX2

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

4xV5-APEX2

Tags

4xV5-APEX2

Expression

Yeast

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Rap2b

Plasmid

#118320

Purpose

Expression of human Rap2b

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

Rap2b (RAP2B Human)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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DT042A

Plasmid

#159403

Purpose

Inducible superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.T5-LacO-6xHis-sfGFP

Tags

6xHis

Expression

Bacterial

Promoter

T5 promoter

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pFF714

Plasmid

#61449

Purpose

IPTG-inducible SCRIBE_galK(OFF)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(OFF)

Use

Synthetic Biology

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.1(-) TRIM12B

Plasmid

#87079

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12B (Trim5 Mouse)

Expression

Mammalian

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pFF706

Plasmid

#61448

Purpose

Light-inducible SCRIBE_kanR(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_kanR(ON)

Use

Synthetic Biology

Promoter

pDawn

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.1(-) TRIM30A

Plasmid

#87081

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM30A (Trim30a Mouse)

Expression

Mammalian

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBetaActin-TUBB2a-mNeonGreen

Plasmid

#191323

Purpose

Expresses fluorescent labeled beta Tubulin to measure microtubule density

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mNeonGreen (Tubb2a Mouse)

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-ERCC1 gRNA1

Plasmid

#211630

Purpose

sgRNA-1 against ERCC1

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ERCC1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-shCIAP2-A

Plasmid

#44130

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

CIAP2

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3 Connexin43-APEX

Plasmid

#44439

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

Insert

Connexin43 fused to pea APEX

Tags

myc

Expression

Mammalian

Mutation

K14D, W41F, E112K mutations on APEX

Promoter

CMV

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAH01SpR

Plasmid

#128159

Purpose

pCAH01 derivative (Ptet inducible promoter) with ahp (kanamycin resistance) CDS replaced with the aadA (streptomycin/spectinomycin resistance) CDS

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Type

Empty backbone

Expression

Bacterial

Promoter

Ptet

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW204

Plasmid

#113244

Purpose

E. coli expression vector for IHFa F13A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa F13A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

F13A

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFF758

Plasmid

#61454

Purpose

IPTG-inducible msd(wt)_dRT

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd(wt)_dRT

Use

Synthetic Biology

Mutation

D197A D198A

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG414GPD-GaCYP722C(pYL777)

Plasmid

#178290

Purpose

Expresses GaCYP722C from G. arboreum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-GaCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

GaCYP722C

Expression

Yeast

Promoter

GAP

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p416PGK1-OsCYP711A2(pYL770)

Plasmid

#178301

Purpose

Expresses OsCYP711A2 from O. sativa in Saccharomyces cerevisiae. Centromeric URA, PPGK1-OsCYP711A2-Tpho5

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

OsCYP711A2

Expression

Yeast

Promoter

PGK1

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXPC3H-Syb37

Plasmid

#123627

Purpose

Expresses Syb37 in e. coli cells, with Arabinose induction and Ampicillin selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

Syb37

Tags

3C-His and PelB

Expression

Bacterial

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
EcAvs2

Plasmid

#188789

Purpose

Avs2 from Escherichia coli NCTC9087

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs2

Expression

Bacterial

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP2367

Plasmid

#160076

Purpose

Z. mobilis CRISPRi, promoter C, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pARiBo4

Plasmid

#98893

Purpose

Expresses ARiBo-fused RNA in vitro with T7 RNA polymerase

Depositor

Pascale Legault

Article

Salvail-Lacoste et al RNA. 2013 Jul;19(7):1003-14. doi: 10.126

Insert

ARiBo4

Use

In vitro transcription vector

Promoter

T7 promoter

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW206

Plasmid

#113246

Purpose

E. coli expression vector for IHFa K15A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa K15A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

K15A

Promoter

T7

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG414GPD-CaCYP722C(pYL803)

Plasmid

#178291

Purpose

Expresses CaCYP722C from C. annuum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-CaCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

CaCYP722C

Expression

Yeast

Promoter

GAP

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG414GPD-LjCYP722C(pYL795)

Plasmid

#178292

Purpose

Expresses LjCYP722C from L. japonicus in Saccharomyces cerevisiae. Centromeric TRP, PGPD-LjCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

LjCYP722C

Expression

Yeast

Promoter

GAP

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG414GPD-VuCYP722C(pYL1070)

Plasmid

#178293

Purpose

Expresses VuCYP722C from V. unguiculata in Saccharomyces cerevisiae. Centromeric TRP, PGPD-VuCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

VuCYP722C

Expression

Yeast

Promoter

GAP

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG414GPD-SlCYP722C(pYL1071)

Plasmid

#178294

Purpose

Expresses SlCYP722C from S. lycopersicum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-SlCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

SlCYP722C

Expression

Yeast

Promoter

GAP

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG414GPD-TpCYP722C(pYL851)

Plasmid

#178295

Purpose

Expresses TpCYP722C from T. pratense in Saccharomyces cerevisiae. Centromeric TRP, PGPD-TpCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

TpCYP722C

Expression

Yeast

Promoter

GAP

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG414GPD-CsCYP722C(pYL877)

Plasmid

#178296

Purpose

Expresses CsCYP722C from C. sinensis in Saccharomyces cerevisiae. Centromeric TRP, PGPD-CsCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

CsCYP722C

Expression

Yeast

Promoter

GAP

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 12,598 results for: 112