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We narrowed to 6,502 results for: poly

Showing: 5651 - 5700 of 6502 results

  2. pCas1_Cas2/3

    Plasmid
    #89240
    Purpose
    Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1
    Depositor
    Blake Wiedenheft
    Article
    Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p
    Insert
    Cas1 and Cas2/3
    Use
    Tags
    Strep II tag on Cas1
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89240-plasmid-map-sequence-id-176381

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  3. MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a

    Plasmid
    #216385
    Purpose
    Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A)
    Depositor
    William Weis
    Article
    Bruguera et al bioRxiv 2024.02.03.578714
    Insert
    Norrin (NDP Human)
    Use
    Baculovirus
    Tags
    GP64 signal sequence-MBP-3C and Rho1D4
    Expression
    Insect
    Mutation
    C93A/C95A/C131A
    Promoter
    Polyhedrin
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    216385-plasmid-map-sequence-id-433265

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  5. SP6-hCas9-Ce-mRNA

    Plasmid
    #47911
    Purpose
    In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.
    Depositor
    Paul Sternberg
    Article
    Chiu et al Genetics. 2013 Aug 26.
    Insert
    hCas9 with C. elegans 5' and 3' UTRs
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    SP6
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47911-plasmid-map-sequence-id-68528

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  11. pDB036d

    Plasmid
    #99216
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants; negative selection
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK, and PproA
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    99216-plasmid-map-sequence-id-198818

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  12. pCS4

    Plasmid
    #55751
    Purpose
    Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.
    Depositor
    Matthew Bennett
    Article
    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50
    Insert
    KanR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-59 of KanR
    Promoter
    T7-lac
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    55751-plasmid-map-sequence-id-86408

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  14. pDB036a

    Plasmid
    #99215
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants; negative selection
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK and PproD
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99215-plasmid-map-sequence-id-192376

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  17. pV2A-T

    Plasmid
    #153022
    Purpose
    Single-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter
    Depositor
    Vito Valiante
    Article
    Hoefgen et al Metab Eng. 2018 Jul;48:44-51. doi: 10.10
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Oct. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    153022-plasmid-map-sequence-id-314021

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  23. pGBW-m4092686

    Plasmid
    #153895
    Purpose
    Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Nearly native encoding;inactivating mutations in …
    Promoter
    Promoter | t7 consensus from NEB, G is +1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153895-plasmid-map-sequence-id-307318

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  24. rat APOBEC1 E63A

    Plasmid
    #112861
    Purpose
    plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells
    Depositor
    Silvestro Conticello
    Article
    Chieca et al bioRxiv 2021
    Insert
    APOBEC1 (Apobec1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Glutamic acid 63 to Alanine
    Promoter
    CMV promoter
    Available since
    Aug. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    112861-plasmid-map-sequence-id-296442

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  30. pRUSH

    Plasmid
    #54479
    Purpose
    Mouse genomic ROSA26 targeting vector, expresses EGFP, U6promoter to drive short RNAs, pgkneo, cassette flanked by loxP sites
    Depositor
    Laurie Jackson-Grusby
    Article
    Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and RNAi
    Tags
    SA-EGFP-polyA, U6 promoter, loxP, and pgk-neo
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  44. Rev7-Halo HRD

    Plasmid
    #207079
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous REV7 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human REV7 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    207079-plasmid-map-sequence-id-471050

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  47. ATM-Halo HRD

    Plasmid
    #207082
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207082-plasmid-map-sequence-id-430992

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Showing: 5651 - 5700 of 6502 results