We narrowed to 13,583 results for: TIM

1. pClneoMyc'-cYAP-1

   Plasmid

   #66854

   Purpose

   Express Myc-tagged C.elegans YAP-1 in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

   Insert

   YAP-1 (yap-1 Nematode)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pClneoMyc'-EGL-44

   Plasmid

   #66855

   Purpose

   Express Myc-tagged C.elegans EGL-44 in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

   Insert

   EGL-44 (CELE_F28B12.2 Nematode)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pONL-N1_ITPKA

   Plasmid

   #65689

   Purpose

   Expresses ITPKA-ONL in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   inositol triphosphate 3-kinase A
   

   Use

   Luciferase

   Tags

   ONL

   Expression

   Mammalian

   Mutation

   contains residues 9-52

   Promoter

   CMV

   Available since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYNL-C1_PTS1

   Plasmid

   #65699

   Purpose

   Expresses YNL-PTS1 in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   peroxisomal targeting signal 1 (tripeptide SKL)
   

   Use

   Luciferase

   Tags

   YNL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCNL-N1_Golgi

   Plasmid

   #65694

   Purpose

   Expresses Golgi-CNL in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   N-terminal 81 amino acids of beta 1,4-galactosyltransferase
   

   Use

   Luciferase

   Tags

   CNL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCNL-N1_TUBB5

   Plasmid

   #65679

   Purpose

   Expresses beta-tubulin-CNL in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   beta-tubulin class V
   

   Use

   Luciferase

   Tags

   CNL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pYNL-N1_Golgi

   Plasmid

   #65693

   Purpose

   Expresses Golgi-YNL in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   N-terminal 81 amino acids of beta 1,4-galactosyltransferase
   

   Use

   Luciferase

   Tags

   YNL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYNL-N1_ITPKA

   Plasmid

   #65687

   Purpose

   Expresses ITPKA-YNL in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   inositol triphosphate 3-kinase A
   

   Use

   Luciferase

   Tags

   YNL

   Expression

   Mammalian

   Mutation

   contains residues 9-52

   Promoter

   CMV

   Available since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCNL-C1_PTS1

   Plasmid

   #65700

   Purpose

   Expresses CNL-PTS1 in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   peroxisomal targeting signal 1 (tripeptide SKL)
   

   Use

   Luciferase

   Tags

   CNL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pONL-C1_PTS1

    Plasmid

    #65701

    Purpose

    Expresses ONL-PTS1 in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    peroxisomal targeting signal 1 (tripeptide SKL)
    

    Use

    Luciferase

    Tags

    ONL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pONL-N1_Mito

    Plasmid

    #65671

    Purpose

    Expresses Mito-ONL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    cytochrome c oxidase subunit VIII
    

    Use

    Luciferase

    Tags

    ONL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNB693

    Plasmid

    #60145

    Purpose

    Disintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    YeLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. refGFP1_pQE30

    Plasmid

    #48644

    Purpose

    Bact exp vector encoding for the 6XHis tagged insensitive variant of the fluorescent redox probe roGFP with mut Cys147 to Serine, del insertion147b and mutated Cys204 to Glut.Referred to as refGFP

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    refGFP1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    refGFP C147S, C204Q

    Promoter

    Available since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. XE94 mFZ4-CS2+

    Plasmid

    #16803

    Depositor

    Randall Moon

    Article

    Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

    Insert

    mFZ4-CS2+ (Fzd4 Mouse)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pUC57-mitoLbNOX

    Plasmid

    #74448

    Purpose

    This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

    Insert

    mitoLbNOX
    

    Use

    Tags

    Flag tag with linker and mitochondrial targeting …

    Expression

    Mutation

    Human codon optimized

    Promoter

    Available since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF1a-hMLH1dn

    Plasmid

    #174824

    Purpose

    Mammalian expression of human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (codon optimized)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of residues 754-756

    Promoter

    EF1a

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCRISPRv2_mSTING_scrambled_gRNA_1

    Plasmid

    #196627

    Purpose

    Scrambled control 1 for STING knock-out in murine cells.

    Depositor

    Nicolas Manel

    Article

    Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

    Insert

    mSTING scrambled gRNA 1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-hSYN-eGFP

    Plasmid

    #162512

    Purpose

    AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (hSYN) promoter

    Depositor

    Bart Nieuwenhuis

    Article

    Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSYN

    Available since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. RGS-6xHis-BLIMP-1-pcDNA3.1-

    Plasmid

    #52518

    Purpose

    expresses human BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    Blimp1 (PRDM1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEB2-mCherry2-L

    Plasmid

    #104003

    Purpose

    Plasmid encoding mCherry2-L

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mCherry2-L
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (MSKGEE NNLA IIKEF… V…

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. lenti U6-sgRNA/EF1a-mCherry

    Plasmid

    #114199

    Purpose

    Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    sgRNA scaffold
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRT029

    Plasmid

    #127969

    Purpose

    double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. lenti EF1a-FLAG-dCas9-VPR

    Plasmid

    #114195

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEIGW Grx1-roGFP2

    Plasmid

    #64990

    Purpose

    Mammalian expression of cytosolic Grx1-roGFP2 (lentiviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LZ10 PBREBAC-H2BHalo

    Plasmid

    #91564

    Purpose

    piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.

    Depositor

    James Zhe Liu

    Article

    Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1

    Insert

    histone H2B-HaloTag
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pOPS0381

    Plasmid

    #133231

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pOPS0382

    Plasmid

    #133232

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with constitutive promoter PsNifH (pOGG043), mCherry (EC15071) and T-pharma (pOGG003)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-HiBiT-CD9

    Plasmid

    #162592

    Purpose

    N-terminally HiBiT-tagged human CD9 under CAG promoter

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

    Insert

    human CD9 (CD9 Human, Synthetic, human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSW003-Pc-mNeonGreen

    Plasmid

    #205018

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    Pc

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-CAG-fDIO-oG-WPRE-SV40pA

    Plasmid

    #74291

    Purpose

    pAAV vector to express oG (optimized Glycoprotein) in a Flp-dependent manner

    Depositor

    Edward Callaway

    Article

    Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

    Insert

    oG (optimized Glycoprotein)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    chimeric glycoprotein

    Promoter

    CAG

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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