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Plasmid#125687PurposeGene knockdown for actinomycetesDepositorInsertstreptomyces codon optimized spdCas9, sgRNA casette
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterermE*/tipAAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBT376
Plasmid#125617PurposeExpresses BE4max-NG in mammalian cellsDepositorInsertBE4max-NG
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRL278
Plasmid#70256PurposesacB plasmid with polylinker; kanamycin/neomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.DepositorTypeEmpty backboneUseConditionally lethalTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-UCHL1
Plasmid#22563DepositorInsertUCHL1 (UCHL1 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBT374
Plasmid#125615PurposeExpresses evoFERNY-BE4max-NG in mammalian cellsDepositorInsertevoFERNY-BE4max-NG
UseTagsExpressionMammalianMutationH102P D104NPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK229
Plasmid#71256Purposeluciferase reposter with 229 bp of the HSV TK promoterDepositorInsertThymidine Kinase promoter
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterHSV Thymidine KinaseAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEMS1938
Plasmid#82563PurposeHigh copy number plasmid containing Ple261 (CLDN5 MiniPromoter) insert.DepositorInsertPle261
UsePleiades promoter project [sic, pleaides plieades]TagsExpressionMutationPromoterAvailable sinceSept. 14, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-Camui-CR
Plasmid#40256DepositorInsertCamuiα-CR
UseTagsExpressionMammalianMutationPromoterCMV IEAvailable sinceOct. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSEVA63_Helper
Plasmid#190432PurposeM13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ297.)DepositorInsertM13 genes: I-XI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAJE-Ma pylRS nitroY/haloY-F5
Plasmid#225684PurposeExpresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.DepositorInsertsM. alvus PylRS 3-NY/HaloY F5
M. alvus Pyl-tRNA(6)
UseMethanomethylophilus alvusTagsExpressionMutationCTG125CTT, N166A, V168S, A223C, W239RPromoterGlnS and LppAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF HaloTag-SYT1
Plasmid#197280PurposeEncodes full-length wild type synaptotagmin 1 (SYT1) with an N-terminal HaloTagDepositorInsertSYT1 and HaloTag (Syt1 Rat)
UseTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFsynW KDEL Hook
Plasmid#197277PurposeEncodes ER-targeted streptavidin-KDEL hook (RUSH system).DepositorInsertStreptavidin
UseLentiviralTagsFLAGExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1(-) mouse C/EBP beta (LIP)
Plasmid#12561DepositorInsertCCAAT/Enhancer-binding Protein beta LIP (Cebpb Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_mNeonGreen2
Plasmid#202566PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal mNeonGreen2 fluorophore on the ORF1 protein for fluorescence microscopyDepositorInsertLINE-1
UseTagsmNeonGreen2 on ORF1pExpressionMammalianMutationPromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBT373
Plasmid#125614PurposeExpresses FERNY-BE4max-NG in mammalian cellsDepositorInsertFERNY-BE4max-NG
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
NRASA
Plasmid#25256PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertNRASA:M1-N172 (NRAS Human)
UseTagsHisExpressionBacterialMutationContains amino acids M1-N172PromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-Ace2_D92N_E199V-mNeon
Plasmid#129256PurposeExpress Ace2_D92N_E199V-mNeon in mammalian cellsDepositorInsertAce2_D92N_E199V-mNeon
UseTagsExpressionMammalianMutationD92N, E199VPromoterCAGAvailable sinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJ123: pRS426-His-MBP-SpuFz1_PSP1
Plasmid#205256PurposeS. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)DepositorInsertSpuFz1
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pST5832
Plasmid#36256DepositorInsertlacZ
UseSynthetic Biology; Mycobacteria replicating vectorTagsExpressionBacterialMutationPromoterModified Hsp60Available sinceNov. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF SYT1-HaloTag
Plasmid#197281PurposeEncodes full-length wild type synaptotagmin 1 (SYT1) with a C-terminal HaloTagDepositorInsertSYT1 and HaloTag (Syt1 Rat)
UseTagsExpressionMammalianMutationN/APromoterEF1alphaAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJKR-O-acuR
Plasmid#62569PurposeAcrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids.DepositorInsertCFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pH6-spTorA-mCherry
Plasmid#169040PurposemCherry preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag in pET28a.DepositorInsertH6-spTorA-mCherry
UseTags6xHis and spTorA (signal peptide of E. coli TorA)ExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:GFP1–10
Plasmid#125663PurposeBinary vector for the inducible expression of GFP1–10 fragment that can be used to detect a protein tagged with GFP11DepositorInsertGFP1–10
UseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B7-deltaE4
Plasmid#122560PurposeModified block 7, with deletion in E4DepositorInsertAdenovirus 5 genomic region 30888-35938
UseSynthetic BiologyTagsExpressionMutationAdenovirus sequences 32874-35461 replaced with Sp…PromoterAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBPHLWL-GAL4DBD
Plasmid#26256DepositorInsertZIP- Gal4DBD (GAL4 Budding Yeast)
UseDrosophila transgenesisTagsExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2853-5 pHR: U6-Sasgv2CXCR4-3 CMV-EGFP
Plasmid#84256PurposeExpresses optimized Sa sgCXCR4-3 gRNA with an EGFP fluorescent markerDepositorInsertSa sgCXCR4-3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pN_35S/mEGFP
Plasmid#132565PurposemEGFP expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)DepositorInsertmEGFP
UseTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35SAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-Gbait-hs-QF2
Plasmid#122563PurposeIntegration of QF2 sequence using the CRISPR/Cas9 system.DepositorInsertGbait-hsp70-QF2-SV40pA
UseCloningTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53_Nsp1
Plasmid#167256PurposeBacterial expression of Nsp1 residues 13-127DepositorInsertNsp1 (residues 13-127) (ORF1ab SARS-CoV-2)
UseTagsHis6-TEV siteExpressionBacterialMutationnonePromoterAvailable sinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pN_35S/mApple
Plasmid#132566PurposemApple expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)DepositorInsertmApple
UseTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35SAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-Gbait-hs-Cre
Plasmid#122562PurposeIntegration of Cre sequence using the CRISPR/Cas9 system.DepositorInsertGbait-hsp70-Cre-SV40pA
UseCloningTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax(TadA, TadA*E59A)
Plasmid#125662PurposepCMV-ABEmax(TadA, TadA*E59A) in mammalian cellsDepositorInsertABEmax(TadA E59A TadA* E59A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
FnR9-10
Plasmid#182567PurposeHuman fibronectin fragment, type-III repeats 9-10, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 9-10
UseTagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1379APromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIF-H1-hpolb-copGFP
Plasmid#23256DepositorInsertDNA polymerase beta shRNA
UseLentiviral and RNAiTagsGFPExpressionMutationPromoterAvailable sinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pET-s34T
Plasmid#28256DepositorInsertsingle chain H3-H4 with Thrombin site
UseTagsHisExpressionBacterialMutationA thrombin cutting site between H3 and H4PromoterAvailable sinceJuly 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV myc DAF-12
Plasmid#32566DepositorInsertDAF-12 (daf-12 Nematode)
UseTagsmyc epitopeExpressionMammalianMutationPromoterCMVAvailable sinceOct. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:ER-GFP1–10
Plasmid#125664PurposeBinary vector for the inducible expression of ER-targeted GFP1–10 fragment that can be used to detect a protein tagged with GFP11DepositorInsertER-GFP1–10
UseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-lacZa
Plasmid#32563DepositorInsertLacZ-alpha
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterConsitutive lacAvailable sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPAN2
Plasmid#79256PurposeExpresses EGFP-tagged DmPAN2 in S2 cellsDepositorInsertDmPAN2 (PAN2 Fly)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only