We narrowed to 4,234 results for: Gca

1. pIRES2-eGFP-Ofd1-Myc-A80T

   Plasmid

   #24579

   Depositor

   Jeremy Reiter

   Article

   Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

   Insert

   Ofd1 (Ofd1 Mouse)
   

   Use

   Tags

   Myc and eGFP

   Expression

   Mammalian

   Mutation

   Alanine 80 changed to threonine (codon GCA to ACA)

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-T11HA-hMOV10-CD

   Plasmid

   #178908

   Purpose

   expression of catalytic dead hMOV10 K530A

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   cDNA Catalytic dead hMOV10 (MOV10 Human)
   

   Use

   Tags

   T11 beta strand of GFP-HA

   Expression

   Mammalian

   Mutation

   K530A catalytic dead AAG>GCA

   Promoter

   CMV protmoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLKO.1-scrCdh2.1 GFP

   Plasmid

   #209100

   Purpose

   GFP expressing scramble of shRNA targeting Cdh2

   Depositor

   Cagla Eroglu

   Article

   Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

   Insert

   scrCdh2.1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MYMH 351

   Plasmid

   #138058

   Purpose

   Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

   Depositor

   Meng How Tan

   Article

   Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

   Insert

   pSEVA-331Bb-10AG 11AC-GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   AAA GAG GAG GCA

   Promoter

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MYMH 336

   Plasmid

   #138057

   Purpose

   Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

   Depositor

   Meng How Tan

   Article

   Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

   Insert

   pSEVA-331Bb-1AG 2AC 11AC-GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GCA GAG GAG ACA

   Promoter

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MYMH 327

   Plasmid

   #138054

   Purpose

   Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

   Depositor

   Meng How Tan

   Article

   Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

   Insert

   pSEVA-331Bb-1AG 2AC-GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GCA GAG GAG AAA

   Promoter

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAFMW-Nbr[K187A]

   Plasmid

   #188593

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Use

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   K187A [AAA=> GCA]

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmStrawberry-Atg4BC74A

   Plasmid

   #21076

   Depositor

   Tamotsu Yoshimori

   Article

   Fujita et al Mol Biol Cell. 2008 Nov . 19(11):4651-9.

   Insert

   Atg4B(C74A) (Atg4b Mouse)
   

   Use

   Tags

   mStrawberry

   Expression

   Mammalian

   Mutation

   220-222 TGC(Cys) is changed to GCA (Ala)

   Promoter

   Available since

   June 8, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TNPO1-shRNA

   Plasmid

   #86082

   Purpose

   A lentiviral construct for building stable cell lines expressing Transportin-1 shRNA via puromycin selection.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Transportin-1 (TNPO1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAFMW-Nbr[R280A,K284A]

    Plasmid

    #188598

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    R280A [CGA=>GCA], K284A [AAG=> GCG]

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFlare9A-Clip2E9 Mutant

    Plasmid

    #209575

    Purpose

    minigene construct for Clip2 E9 alternative splicing, potential PTBP2 binding sites are deleted

    Depositor

    Sika Zheng

    Article

    Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

    Insert

    Clip2 (Clip2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ACGCGTTTCTGAATTCTTCTAACTGCCCTCAAATGCACGGTGGCATGTG…

    Promoter

    CMV

    Available since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Pmyo-3::hpo-30 gDNA (pBAB204)

    Plasmid

    #117385

    Purpose

    Allows for body-wall muscle expression of HPO-30

    Depositor

    Kavita Babu

    Article

    Sharma et al J Neurosci. 2018 Aug 8;38(32):7072-7087.

    Insert

    genomic DNA of hpo-30 (833 bp)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRA03

    Plasmid

    #103141

    Purpose

    Triple gRNA expression separated with 28nt Csy4 motif

    Depositor

    Jens Nielsen

    Article

    Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

    Insert

    gRNAs
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-hSyn-mCherry-Satb2 sesRNA-2a-msFlag-2a-tTA-WPRE

    Plasmid

    #239028

    Purpose

    Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for mouse Satb2 (Satb2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUDP123

    Plasmid

    #107269

    Purpose

    pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUDP044

    Plasmid

    #101168

    Purpose

    pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Insert

    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.4_CEACAM6-His

    Plasmid

    #149337

    Purpose

    for eukaryotic expression and purification of CEACAM6-His6

    Depositor

    James Fleckenstein

    Article

    Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

    Insert

    CEACAM6 (CEACAM6 Human)
    

    Use

    Tags

    IL-2 signal peptide and polyhistidine

    Expression

    Mammalian

    Mutation

    point mutation (GGA to GCA) to replace 'G-g…

    Promoter

    CMV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSUPER-hHSF2 shRNA

    Plasmid

    #118346

    Purpose

    This plasmid expresses shRNA against hHSF2.

    Depositor

    Lea Sistonen

    Article

    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

    Insert

    hHSF2 shRNA (HSF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAFMW-Nbr[R272A,K276A, R280A,K284A]

    Plasmid

    #188599

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    R272A [CGG=> GCG], K276A [AAG=>GCG], R280A …

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSBK.016

    Plasmid

    #187217

    Purpose

    Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 8 [GCT-TGC]), and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35 (long a1/a2, barcode 8 [GCA-TGC])
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7/Lac

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSLQ7535 pHR (hU6-crSURF2-EFS-PuroR-WPRE)

    Plasmid

    #214879

    Purpose

    Lentiviral vector encoding RfxCas13d targeting SURF2 guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crSURF2-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pWT029d

    Plasmid

    #96859

    Purpose

    (d)guanine _agRNA (GFP activation)

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    (d)guanine _agRNA (GFP activation)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pWT029c

    Plasmid

    #96858

    Purpose

    guanine-agRNA (GFP activation)

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    guanine-agRNA (GFP activation)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pWT029g

    Plasmid

    #96860

    Purpose

    guanine-agRNA (GFP activation, 18 nt blocker with bulges)

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    guanine-agRNA (GFP activation, 18 nt blocker with bulges)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pWT029h

    Plasmid

    #96861

    Purpose

    (d)guanine-agRNA (GFP activation,18 nt blocker with bulges)

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    (d)guanine-agRNA (GFP activation,18 nt blocker with bulges)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. OA-1050I (notch)

    Plasmid

    #132421

    Purpose

    express arrays of gRNA targeting Notch under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    notch gRNA array
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6-3

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJK01_Rho_minprox_DsREd

    Plasmid

    #173489

    Purpose

    PCR template for reporter gene with mouse Rhodopsin promoter with DsRed

    Depositor

    Barak Cohen

    Article

    White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

    Insert

    Mouse Rhodopsin promoter driving DsRed reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Rhodopsin

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Circular 200,100 (GAPDH)

    Plasmid

    #170120

    Purpose

    AAV vector carrying a guide RNA targeting the human GAPDH mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA (GAPDH Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Circular 200,100 with interspersed loops (GAPDH)

    Plasmid

    #180183

    Purpose

    AAV vector carrying a guide RNA targeting the human GAPDH mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with interspersed loops (GAPDH Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Circular 200,100 with 8 bp bulge loops (GAPDH)

    Plasmid

    #170121

    Purpose

    AAV vector carrying a guide RNA targeting the human GAPDH mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with 8bp bulge loops (GAPDH Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-hSyn-mCherry-Plxnd1 sesRNA-2a-msFlag-2a-tTA2-WPRE

    Plasmid

    #239032

    Purpose

    Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for mouse PlxnD1 (Plxnd1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. TRE-mClover3-TDP-43ΔNLS

    Plasmid

    #214917

    Purpose

    inducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear puncta

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    TDP-43ΔNLS (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    mClover3

    Expression

    Mutation

    mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. TRE-TDP-43ΔNLS-mClover3

    Plasmid

    #214916

    Purpose

    inducible expression of TDP-43 harboring mutant NLS and C-terminal mClover3. Expression yields cytosolic diffuse TDP-43

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    TDP-43ΔNLS (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    mClover3

    Expression

    Mutation

    mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

    Promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PGL3-U6-RNF2_pegRNA-Csy4RS-nick_sgRNA-EGFP

    Plasmid

    #172669

    Purpose

    For expression of transcript containing pegRNA and nick-sgRNA targeting human RNF2 gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

    Insert

    RNF2 pegRNA and RNF2_nick-sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSLQ7534 pHR (hU6-crSURF1-EFS-PuroR-WPRE)

    Plasmid

    #214878

    Purpose

    Lentiviral vector encoding RfxCas13d targeting SURF1 guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crSURF1-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCoHygro L16_shRNA of spliced Copia

    Plasmid

    #71389

    Purpose

    knock down expression of spliced Copia in Drosophila melanogaster

    Depositor

    Timothy Nilsen

    Insert

    Copia
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAM/CBA-eGFP-miRNegativex3-WPRE-bGHpA

    Plasmid

    #194249

    Purpose

    Expresses 3 non-targeting miRNAs

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    miRNegative
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. OgeuIscB CAG RNA

    Plasmid

    #222867

    Purpose

    This plasmid codes for the guide of the OgeuIscB with a CAG target

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    OgeuIscB omega RNA with a (CAG)6 target sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Oct. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pWT016i

    Plasmid

    #96856

    Purpose

    theophylline-agRNA-GFP

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    theophylline-agRNA-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pWT016l

    Plasmid

    #96857

    Purpose

    (d)theophylline-agRNA-GFP

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    (d)theophylline-agRNA-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pWT055b

    Plasmid

    #96877

    Purpose

    theophylline-agRNA-FANCF

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    theophylline-agRNA-FANCF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCPP5912

    Plasmid

    #128703

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

    Insert

    shcE-avrE1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Three synonymous mutations Ala 362 (GCT-GCC), Ala…

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. SL2-sgSat-MTSb/BFP/pdCas9-C1

    Plasmid

    #162761

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting centromeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgSat-MTSb)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. PGL3-U6-ALDOB_pegRNA-Csy4RS-nick_sgRNA-EGFP

    Plasmid

    #172668

    Purpose

    For expression of transcript containing pegRNA and nick-sgRNA targeting human ALDOB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

    Insert

    ALDOB pegRNA and ALDOB_nick-sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pUDP010

    Plasmid

    #101166

    Purpose

    E. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Insert

    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pUDP012

    Plasmid

    #101167

    Purpose

    E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Insert

    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. Circular 200,100 (mPCSK9)

    Plasmid

    #170122

    Purpose

    AAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promoters

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA (Pcsk9 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and mouse U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pk335-CARGO-11mer-35kb-DSF-1-11

    Plasmid

    #227489

    Purpose

    11-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 35kb Downstream Fgf5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pLCHKO_TK1_HPRT1_Lb_6

    Plasmid

    #155064

    Purpose

    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pAAV-hSyn-mCherry-VGAT sesRNA-2a-msFlag-2a-tTA2-WPRE

    Plasmid

    #239030

    Purpose

    Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for mouse VGAT (Slc32a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge