178,651 results
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Plasmid#11911DepositorInsertpRSETA-PAGFP
ExpressionBacterialMutationThe PAGFP has four mutations (L64F/T65S/V163A/T20…Available sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET17_K560_GFP_His
Plasmid#15219DepositorPublicationAvailable sinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMXs-Sall4
Plasmid#13361DepositorInsertsal-like 4 (Drosophila) (Sall4 Mouse)
UseRetroviralExpressionMammalianMutation*(see below)Available sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro Rb shRNA
Plasmid#10670DepositorAvailable sinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV/mIba1.GFP.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA
Plasmid#190163PurposeMicroglia-targeted transgene expression.DepositorInsertsmIba1 promoter, 1.7-kb, microglia-specific promoter
miR-9-Tx4, 4 repeated miRNA target sites for miR-9
miR-129-2-3p-Tx4, 4 repeated miRNA target sites for miR-129-2-3p
UseAAVAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJR104
Plasmid#187243PurposeLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expressionDepositorInsertLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
UseLentiviralAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJR98
Plasmid#187239PurposeCR3 constant region - hU6 sgRNA promoter flanked by BsmBI sitesDepositorInsertCR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
ExpressionBacterialAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
YAP/TAZ-TRE-mStrawberry reporter
Plasmid#158682PurposeThis plasmid is a YAP/TAZ pathway reporter. It has a YAP/TAZ-responsive synthetic promoter driving the expression of mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralExpressionMammalianAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-TO-ASCL1-DLX2
Plasmid#182307PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expressionTagsNone and T2A-mycNLS-mTagBFP2ExpressionMammalianPromoterEF1a and pTRE3G-bidirectionalAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Olig001
Plasmid#170716PurposeOlig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNSDepositorInsertOlig001 capsid gene / rep 2
UseAAVAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cbh_v5 AAV-CBE C-terminal
Plasmid#137176PurposeAAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE C-terminal; U6-protospacer
UseAAVPromoterCbhAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-Sp-pegRNA-RNF2_+5GtoT
Plasmid#135957PurposeS. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRNF2_5GtoT pegRNA
ExpressionMammalianMutationSee manuscriptPromoterU6Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLH01
Plasmid#117261PurposeGenome editing for Lactobacillus plantarum WCSF1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPorfX-Lp_0640-42, sapK-sappR
UseOtherAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lentiviral-S-tdTomato
Plasmid#112579PurposeExpressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vectorDepositorInsertNLS-tdTomato-NES
UseLentiviralTagsnuclear export sequence and nuclear localization …ExpressionMammalianAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE
Plasmid#104495PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switchDepositorHas serviceAAV1, AAV PHP.eB, and AAV RetrogradeInsertjGCaMP7s
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianPromoterCAGAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-EGFP
Plasmid#89684PurposepCAG promoter driving EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP
UseSynthetic BiologyExpressionMammalianAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLENTI6.3/AR-GC-E2325
Plasmid#85128Purposeexogenous AR expressionDepositorAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pY094
Plasmid#84743PurposeExpresses huAsCpf1-T2A-GFP and crRNA guideDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertshuAsCpf1
GFP
Tags3xHA, NLS, and T2AExpressionMammalianPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAVS1-Pur-CAG-EGFP
Plasmid#80945Purposehuman AAVS1 site targeting donor plasmid for knocking-in EGFP expression cassette driven by CAG promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP
ExpressionMammalianPromoterCAG promoterAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmiRFP703-N1
Plasmid#79988PurposeMonomeric near-infrared fluorescent protein miRFP703 in mammalian plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmiRFP703
ExpressionMammalianPromoterCMVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA
Plasmid#73796PurposepRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNADepositorInsertsdCas9-Mxi1
Tet Repressor
Structural gRNA for S pyogenes
UseCRISPRTagsMxi1 and NLSExpressionYeastMutationD10A, H840APromoterpGPM1, pRPR1(TetO), and pTef1Available sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK291 (mAID-mClover-Bsr)
Plasmid#72829PurposeC-terminal tagging with mAID-mClover using CRISPR/Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmAID-mClover-Bsr
UseCRISPRTagsmAID-mCloverExpressionMammalianAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAS1NB c Rosella I
Plasmid#71245Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.DepositorInsertRosella
ExpressionYeastPromoterPGK1Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCW57.1-FOXM1c
Plasmid#68810PurposeDoxycycline inducible lentiviral vector for FOXM1c expression in mammalian cellsDepositorPublicationInsertFOXM1c (FOXM1 Human)
UseLentiviral; Doxycycline inducibleTagsFLAGExpressionMammalianPromoterTRE promoter, Tet ONAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-Sec16A
Plasmid#36155PurposeExpresses Sec16A as a GFP fusionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSec16A (SEC16A Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSBbi-Hyg
Plasmid#60524PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAC-LYC
Plasmid#53270PurposeContains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coliDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcrtE, crtI, crtB
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-GFAP104-mCherry
Plasmid#58909PurposeAAV-mediated expression of mCherry under the GFAP promoterDepositorHas serviceAAV5InsertmCherry
UseAAVExpressionMammalianPromoterGFAPAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNestin-EGFP
Plasmid#38777DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnestin 2nd intron enhancer with TK mini promoter (NES Human)
TagsEGFPExpressionMammalianAvailable sinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
tetO-GATA4
Plasmid#46030DepositorInsertGATA4 (GATA4 Human)
UseLentiviralTagsHis and V5ExpressionMammalianMutationR43Q, G50S and N197SPromotertetOAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by