165,947 results

1. pLifeAct-miRFP670nano

   Plasmid

   #127434

   Purpose

   F-Actin labeling with near-infrared fluorescent protein miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   LifeAct-miRFP670nano
   

   Tags

   miRFP670nano

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGGD001

   Plasmid

   #48833

   Purpose

   Provides linker:GFP as GreenGate C-terminal tag module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   Linker:GFP
   

   Use

   Golden gate compatible cloning vector

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBEST-p15A-PLtetO1-tetR-T500

   Plasmid

   #45391

   Depositor

   Vincent Noireaux

   Article

   Shin et al J Biol Eng. 2010 Jun 24;4:8.

   Insert

   tet repressor
   

   Expression

   Bacterial

   Promoter

   PL-tetO1

   Available Since

   June 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSIE1

   Plasmid

   #136355

   Purpose

   Selection by Inducible Effectors

   Depositor

   Andrew Goodman

   Article

   Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB

   Inserts

   Type VI effector bfe1
   Type VI effector bte1
   BT_4676
   tetR
   

   Use

   Unspecified

   Mutation

   ATG removed to add signal sequence before gene

   Promoter

   P1TDP-GHO23

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221_SLC30A8

   Plasmid

   #132136

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC30A8 (SLC30A8 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pICH-chl-roGFP2-ORP1

   Plasmid

   #191699

   Purpose

   Ti plasmid pICH86988 harbouring chl-roGFP2-ORP1 for peroxide detection in the plastids of plant cells.

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   chl-roGFP2-ORP1
   

   Use

   Binary agrobacterium ti plasmid

   Expression

   Plant

   Promoter

   CaMV 35S promoter

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

   Plasmid

   #185910

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8e-nSpCas9-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A

   Promoter

   CMV and T7

   Available Since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET32a-hHBEGF

   Plasmid

   #199234

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Proheparin-binding EGF-like growth factor (HBEGF Human)
   

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHW539 (15xUAS::kin-2a(G310D)::SL2::GFP::let-858 3'UTR)

   Plasmid

   #107137

   Purpose

   Dominant negative PKA cGAL effector for C. elegans

   Depositor

   Paul Sternberg

   Article

   Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

   Insert

   kin-2a(G310D)::SL2::GFP
   

   Expression

   Worm

   Promoter

   15xUAS-delta-pes-10(basal promoter)

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMZ828

    Plasmid

    #155322

    Purpose

    Constitutive expression of PhyB(1-650)-VP16 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-PhyB(1-650)-VP16-NLS-Tnos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pROF021

    Plasmid

    #155326

    Purpose

    Expression of PULSE-controlled FLuc

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    (etr)8-(C120)5-PhCMVmin-FLuc-TSV40
    

    Use

    Luciferase and Synthetic Biology; Eukaryotic expr…

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFETCh_RXRB

    Plasmid

    #104040

    Purpose

    Donor vector for 3' FLAG tag of human RXRB

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    RXRB homology arms (RXRB Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mammalian

    Available Since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-DIO-TC66T-2A-oG

    Plasmid

    #178430

    Purpose

    Expresses TVA(TC66T) and optimized Rabies G (oG) protein in a FLEX cassette

    Depositor

    Edward Callaway

    Inserts

    TVA950 Glu66→Thr
    Optimized G
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Promoter

    hSyn

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFTK048

    Plasmid

    #171320

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    DsRed.T1-SKL fluorescent reporter
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Arp3-pmCherryC1

    Plasmid

    #27682

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Arp3 (ACTR3 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEf1a-goldDn29-P2A-GFP

    Plasmid

    #247153

    Purpose

    Mammalian expression of a human codon optimized engineered goldDn29 recombinase (efficient and specific variant)

    Depositor

    Patrick Hsu

    Article

    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

    Insert

    goldDn29-P2A-GFP
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N

    Promoter

    Ef1a

    Available Since

    Nov. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-C1-DNAJC13(FL)

    Plasmid

    #246660

    Purpose

    Mammalian expression of full length, eGFP tagged DNAJC13

    Depositor

    Braden Lobingier

    Article

    Adoff et al Mol Biol Cell. 2025 Sep 1;36(9):ar114. d

    Insert

    DNAJC13 (DNAJC13 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. CRAF-HiBiT Fusion Vector

    Plasmid

    #238595

    Purpose

    Express CRAF-HiBiT Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    CRAF (RAF1 Human)
    

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. HCT163_pAAV-eHGT_3Xcore_1137m-minBGprom-ChR2(H134R)-EYFP-WPRE3-bGHpA

    Plasmid

    #233562

    Purpose

    Direct-expressing ChR2(H134R)-EYFP AAV virus

    Depositor

    Tanya Daigle, Allen Institute for Brain Science

    Article

    Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV

    Promoter

    minBglobin

    Available Since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF1607

    Plasmid

    #144368

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BAZ2B (BAZ2B Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pPMS-987

    Plasmid

    #232280

    Purpose

    Protein Expression

    Depositor

    Jeremy Nathans

    Insert

    Antrx2 (Antxr2 Mouse)
    

    Expression

    Mammalian

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pGL4.26-Ccnd2PromoterRegion1(1.2kb)

    Plasmid

    #228058

    Purpose

    1.2 kb from mouse Ccnd2 promoter in front of firefly luciferase reporter

    Depositor

    Kenneth Chen

    Article

    Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

    Insert

    Ccnd2 promoter (Ccnd2 Mouse)
    

    Use

    Luciferase

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pTRIPZ-shWT1_B

    Plasmid

    #217637

    Purpose

    Dox inducible shRNA targeting WT-1

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    shWT1_B (WT1 Human)
    

    Use

    RNAi

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCR8-PspCas13b_gRNA[ccdbCam]_1xMS2b

    Plasmid

    #196847

    Purpose

    backbone for gRNA cloning of dpspCas13b tagged by 1xMS2

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pzk408-pmax-3xflag-dpspCas13b(AAAA)-NES

    Plasmid

    #196828

    Purpose

    Express dpdpCas13b with AAAA mutation

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Insert

    dpspCas13b(AAAA)
    

    Tags

    3XFLAG and NES

    Expression

    Mammalian

    Mutation

    K367A, D369A and K370A

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pKB238

    Plasmid

    #170033

    Purpose

    Encodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Neuropeptide Y (NPY Synthetic, Human, Escherichia coli)
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Promoter

    Ptac and PvirK

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pHH0103_RAPGEF4_DEP

    Plasmid

    #109895

    Purpose

    Protein expression and purification of RAPGEF4_DEP

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    RAPGEF4_DEP (RAPGEF4 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. 6379 H10-K218T

    Plasmid

    #1250

    Depositor

    Susan Lindquist

    Article

    Glover et al Cell 1998 Jul 10;94(1):73-82.

    Insert

    Hsp104 (K218T) (HSP104 Budding Yeast)
    

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    K218T

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

29. 6380 H10-K620T

    Plasmid

    #1251

    Depositor

    Susan Lindquist

    Article

    Glover et al Cell 1998 Jul 10;94(1):73-82.

    Insert

    Hsp104 (K620T) (HSP104 Budding Yeast)
    

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    K620T

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

30. hKDR

    Plasmid

    #83436

    Purpose

    human VEGFR2 with V5 and tGFP tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Am J Physiol Cell Physiol. 2018 Aug 1;31

    Insert

    human VEGFR2 (KDR Human)
    

    Tags

    V5 and turboGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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