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4,133 results

Showing: 3601 - 3630 of 4133 results

  2. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51085
    Purpose
    Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV Retrograde and AAV1
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin1
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51085-plasmid-map-sequence-id-148706

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  4. pDULE-ABK

    Plasmid
    #49086
    Purpose
    A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments
    Depositor
    Peter Schultz
    Article
    Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.
    Inserts
    tRNA synthetase
    tRNA for a photocrosslinking amino acid
    Use
    Tags
    Expression
    Bacterial
    Mutation
    L274M, C313A, Y349F
    Promoter
    GlnRS and lpp
    Available since
    Nov. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    49086-plasmid-map-sequence-id-396431

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  7. pRK5-myc-RhoA-Q63L

    Plasmid
    #12964
    Depositor
    Gary Bokoch
    Insert
    RhoA constitutively active (RHOA Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Q63L Constitutively active
    Promoter
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only
    12964-plasmid-map-sequence-id-139200

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  12. pmEGFP-1

    Plasmid
    #36409
    Depositor
    Benjamin Glick
    Type
    Empty backbone
    Use
    Fluorescent protein cassette for sub-cloning
    Tags
    mEGFP
    Expression
    Mutation
    Promoter
    no promoter
    Available since
    May 15, 2012
    Availability
    Academic Institutions and Nonprofits only
    36409-plasmid-map-sequence-id-45554

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  13. pSico PGK puro

    Plasmid
    #11586
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes the puromycin gene to be recombined out of the construct, activating shRNA expression.
    Depositor
    Tyler Jacks
    Article
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
    11586-plasmid-map-sequence-id-204172

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  18. Luc.Cre empty vector

    Plasmid
    #20905
    Depositor
    Tyler Jacks
    Type
    Empty backbone
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 22, 2009
    Availability
    Academic Institutions and Nonprofits only
    20905-plasmid-map-sequence-id-339882

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  23. pDONR207 SARS-CoV-2 M

    Plasmid
    #141274
    Purpose
    Gateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Has Service
    Cloning Grade DNA
    Insert
    M (M SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    April 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141274-plasmid-map-sequence-id-288102

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  27. GFP-BLM

    Plasmid
    #80070
    Purpose
    Expresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)
    Depositor
    Nathan Ellis
    Article
    Hu et al Hum Mol Genet. 2001 Jun 1;10(12):1287-98
    Insert
    BLM (BLM Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    If correct ID, the cDNA is from HeLa and has char…
    Promoter
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    80070-plasmid-map-sequence-id-423157

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  28. pdCas9-DNMT3A-PuroR_v2

    Plasmid
    #74407
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74407-plasmid-map-sequence-id-140317

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Showing: 3601 - 3630 of 4133 results