We narrowed to 29,740 results for: REP
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Plasmid#222404Purposearabinose-induced synthesis of a hybrid RNA containing a 5' MS2 hairpin, E. coli chiP 5'UTR insert between XmaI and HindIII sites, surrounded by a 7bp GC clamp, and followed by a 3' trpA terminator.DepositorInsert5’UTR of chiP (-98 to +12, relative to AUG) (chiP E. coli)
UseTags1xMS2 hairpin, 7GC clamp, TtrpA terminatorExpressionBacterialMutationPromoterpBADAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
3xMS-TR-100-PURO HRD
Plasmid#207582Purposehomologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locusDepositorInsert3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
UseTags3xMS2ExpressionMammalianMutationPromoterSV40Available SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H56A)
Plasmid#231373PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationH56APromoterCMVAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R68A)
Plasmid#231374PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationR68APromoterCMVAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R97A)
Plasmid#231375PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationR97APromoterCMVAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(K98A)
Plasmid#231376PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationK98APromoterCMVAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H56A, K98A)
Plasmid#231377PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationH56A, K98APromoterCMVAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R97A, K98A)
Plasmid#231378PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationR97A, K98APromoterCMVAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R97E, K98E)
Plasmid#231380PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationR97E, K98EPromoterCMVAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R710A)
Plasmid#231381PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationR710APromoterCMVAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H712A)
Plasmid#231382PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationH712APromoterCMVAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R710A, H712A)
Plasmid#231383PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationR710A, H712APromoterCMVAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R635A)
Plasmid#231384PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationR635APromoterCMVAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(D669A)
Plasmid#231371PurposeExpress catalytically dead mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationD669APromoterCMVAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
hnRNPAB X2-IDR
Plasmid#216517PurposeExpresses MBP-tagged intrinsically disordered region of hnRNPAB X2DepositorInserthnRNPAB X2-IDR
UseTags6xHis tags-MBPExpressionBacterialMutationdeleted amino acids 2-225PromoterAvailable SinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRRv2_sgTom
Plasmid#218346PurposeCRISPR KO plasmid for tdTomato in human cell linesDepositorInsertInserted sgRNA targetting tdTomato
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tensin-2 Y483E
Plasmid#201794PurposeExpresses Tensin-2 protein with Y483E mutation in MAC-Tag-C backboneDepositorInsertTensin-2 (Tns2 Mouse)
UseTagsBirA, HA, strepIIIExpressionBacterial and MammalianMutationmutation Y483EPromoterAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tensin-2 Y483F
Plasmid#201795PurposeExpresses Tensin-2 protein with Y483F mutation in MAC-Tag-C backboneDepositorInsertTensin-2 (Tns2 Mouse)
UseTagsBirA, HA, strepIIIExpressionBacterial and MammalianMutationmutation Y483FPromoterAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tensin-2 Y483A
Plasmid#201796PurposeExpresses Tensin-2 protein with Y483A mutation in MAC-Tag-C backboneDepositorInsertTensin-2 (Tns2 Mouse)
UseTagsBirA, HA, strepIIIExpressionBacterial and MammalianMutationmutation Y483APromoterAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-TSen
Plasmid#209916PurposeA FRET biosensor for ADAM17 activityDepositorInsertTSen
UseTagsECFP and YPetExpressionMammalianMutationPromoterAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9 fused to scFv (anti-spike)
Plasmid#186421PurposeExpression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2DepositorInsertdCas9-scFv fusion (anti-SARS-CoV-2 spike)
UseCRISPRTags6HisExpressionMutationPromoterAvailable SinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-FLuc0_EMCV-Ren
Plasmid#210590PurposeExpresses SM(FLAG)-nonoptimal Firefly luciferase, and EMCVIRES-driven Renilla luciferaseDepositorInsertsSpaghetti monster (FLAG)-nonoptimal FLuc
Optimal Renilla luciferase
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterEMCV IRES and UBC humanAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-FLuc100_EMCV-Ren
Plasmid#210591PurposeExpresses SM(FLAG)-optimal Firefly luciferase, and EMCVIRES-driven Renilla luciferaseDepositorInsertsSpaghetti monster (FLAG)-optimal FLuc
Optimal Renilla luciferase
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterEMCV IRES and UBC humanAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-yhaM-RFP
Plasmid#207651PurposeOverexpression of native L-cysteine desulfidase and RFP in E. coli MG1655DepositorInsertyhaM
UseTags6xHisExpressionBacterialMutationPromoterT7Available SinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-PES-H1-L92F-Q94Y
Plasmid#218705PurposeEncodes codon optimized PES-H1 L92F-Q94Y enzyme for bacterial expressionDepositorInsertPES-H1
UseTags10xHisExpressionBacterialMutationL92F-Q94YPromoterAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGA-pTRE-B
Plasmid#218894Purposegolden gate cloningDepositorInsertTRE
UseSynthetic BiologyTagsExpressionMutation580G>CPromoterAvailable SinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGF-WPRE-G
Plasmid#218901Purposegolden gate cloningDepositorInsertWPRE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGE-V5_TurboID-F
Plasmid#218900Purposegolden gate cloningDepositorInsertV5-TurboID
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGD_MUTT2A_E
Plasmid#218899Purposegolden gate cloningDepositorInsertMUTT2A
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGD-T2A-E
Plasmid#218898Purposegolden gate cloningDepositorInsertT2A
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only