We narrowed to 5,173 results for: pcDNA3.1+

1. pmyc-GFP-TNRC6A-del.GW-I+II+III-NES-mut

   Plasmid

   #42040

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   deleted aa457-532, aa716-772 and aa804-924, F951A…

   Promoter

   CMV

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. mcherry Beta 4

   Plasmid

   #36114

   Depositor

   Narasimhan Gautam

   Article

   Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

   Insert

   G protein beta subunit B4 (Gnb4 Mouse)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   May 3, 2012

   Availability

   Academic Institutions and Nonprofits only

3. mcherry Beta 3

   Plasmid

   #36113

   Depositor

   Narasimhan Gautam

   Article

   Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

   Insert

   G protein beta subunit B3 (GNB3 Human)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   May 3, 2012

   Availability

   Academic Institutions and Nonprofits only

4. CMV-2xFLAG-Atoh1ER-DsRed

   Plasmid

   #101735

   Purpose

   Expresses mouse Atoh1 fused to ER and DsRed

   Depositor

   Albert Edge

   Article

   Parker et al Hum Gene Ther Methods. 2014 Feb;25(1):1-

   Insert

   Atoh1 (Atoh1 Mouse)
   

   Use

   Tags

   2x FLAG, DsRed, and ER

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPA-RL-FL

   Plasmid

   #78863

   Purpose

   DULIP Tandem Construct used for data normalization

   Depositor

   Erich Wanker

   Article

   Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

   Insert

   Firefly
   

   Use

   Expression vector

   Tags

   Protein A, Renilla luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTS1026-Tier1-PhCMV-3xNLS-iRFP670

   Plasmid

   #169532

   Purpose

   Tier-1 vector encoding PhCMV-driven iRFP670 that is localized to the nucleus (PhCMV-3xNLS-iRFP670-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven nucleus-localized far-red fluorescent protein iRFP670
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA-NES-ZapCmR1.1

   Plasmid

   #59014

   Purpose

   Cytosolic zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

   Insert

   NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
   

   Use

   Tags

   Nuclear Exclusion Signal

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

8. a5-ecto-pcDNA31hygro

   Plasmid

   #113386

   Purpose

   Express human alpha5 ectodomain with secretion peptide, purification tags and C-terminal clasp

   Depositor

   Timothy Springer

   Article

   Li et al EMBO J. 2017 Mar 1;36(5):629-645. doi: 1

   Insert

   human alpha 5 ectodomain
   

   Use

   Tags

   Strep II tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YFP Gamma 12

   Plasmid

   #36109

   Depositor

   Narasimhan Gautam

   Article

   Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

   Insert

   G protein subunit Gamma 12 (Gng12 Mouse)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 3, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pFL-V5-GW

    Plasmid

    #78857

    Purpose

    DULIP Prey Destination Vector (Gateway, N-terminal)

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Firefly luciferase, V5-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPA-RL-GW

    Plasmid

    #78855

    Purpose

    DULIP Bait Destination Vector (Gateway, N-terminal)

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Protein A-Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. V5-WT-short-UBASH3A

    Plasmid

    #192088

    Purpose

    Mammalian expression of human wild-type short-form UBASH3A with V5 tag

    Depositor

    Patrick Concannon

    Article

    Ge et al Diabetes. 2017 Jul;66(7):2033-2043. doi:

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-BAP-Sox2

    Plasmid

    #133281

    Purpose

    Expression of target proteins BAP-Sox2 in mammalian cells

    Depositor

    Arman Kulyyassov

    Article

    Kulyyassov et al Biomolecules. 2020 Jan 16;10(1). pii: bi

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Tags

    Biotin Acceptor Peptide contains 7-His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTS2311-Tier1-spacer-PPGK-NLuc_TB

    Plasmid

    #169552

    Purpose

    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-NanoLuc Luciferase - mTagBFP fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. YFP-Gamma 5

    Plasmid

    #36044

    Depositor

    Narasimhan Gautam

    Article

    Akgoz et al Cell Signal. 2006 Oct;18(10):1758-68. Ep

    Insert

    G protein subunit Gamma 5 (Gng5 Rat)
    

    Use

    Tags

    His and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pActin-miRFP718nano

    Plasmid

    #195745

    Purpose

    Human beta-actin labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    miRFP718nano-human beta-actin
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGW-FL-V5

    Plasmid

    #78858

    Purpose

    DULIP Prey Destination Vector (Gateway, C-terminal)

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Firefly luciferase, V5-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGW-RL-PA

    Plasmid

    #78856

    Purpose

    DULIP Bait Destination Vector (Gateway, C-terminal)

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Renilla luciferase, Protein A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-CAML-Myc-His

    Plasmid

    #22295

    Depositor

    Erik Lillehoj

    Article

    Guang et al Int J Biochem Cell Biol. 2009 Jun . 41(6

    Insert

    calcium modulating ligand (CAMLG Human)
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pTS2381-Tier1-PhCMV-Igk_TM

    Plasmid

    #169487

    Purpose

    Tier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) for membrane localization of fusion proteins attached to a secretion signal Igk (PhCMV-IgkSS-TM-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven membrane localization
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNIR-Fb6m

    Plasmid

    #184680

    Purpose

    NIR-Fb to spike-Cov-19 RBD

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-Fb6m (S Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-HA-REDD1 P139A (#625)

    Plasmid

    #99945

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    P139A

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-HA-REDD1 S137A (#627)

    Plasmid

    #99943

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S137A

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. YFP Gamma 4

    Plasmid

    #36104

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 4 (Gng4 Mouse)
    

    Use

    Tags

    YFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pcDNA3 RL3Am

    Plasmid

    #109364

    Purpose

    Human rod opsin chimera with intracellular loop 3 from Amphioxus opsin 1 with 1D4 tag

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Rod opsin chimera with Amphioxus Opsin1 intracellular Loop 3 (RHO Branchiostoma belcheri, Human)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Opto-casp9_casp9-VfAU1-LOV

    Plasmid

    #131222

    Purpose

    Expresses Opto-casp9_casp9-VfAU1-LOV (light-activated caspase9)

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    Opto-casp9 (casp9-VfAU1-LOV)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Opto-casp9_casp9-AtCRY2-PHR

    Plasmid

    #131223

    Purpose

    Opto-casp9_casp9-AtCRY2-PHR (light-activated caspase9)

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    Opto-casp9 (casp9-AtCRY2-PHR)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCLC3sFlag

    Plasmid

    #52422

    Purpose

    mammalian expression of flag tagged rat CLC3 short transcript

    Depositor

    Steven Weinman

    Article

    Shimada et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    CLC3 (Clcn3 Rat)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. b2-TS5

    Plasmid

    #103878

    Purpose

    full-length human b2 with tension sensor at b2_743

    Depositor

    Timothy Springer

    Article

    Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

    Insert

    full-length human b2 with tension sensor at b2_743 (ITGB2 Human)
    

    Use

    Tags

    tension sensor module

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGlomyc-Frat1

    Plasmid

    #124499

    Purpose

    Expresses myc-tagged mouse FRAT1 in mammalian cells (N-terminal tag)

    Depositor

    Renee van Amerongen

    Article

    van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

    Insert

    Frat1 (Frat1 Mouse)
    

    Use

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge