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We narrowed to 6,626 results for: GFP expression plasmids

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Showing: 6251 - 6300 of 6626 results

  2. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201152
    Purpose
    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    dCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Mutation
    gRNA sequence: TACGTTCTCTATCACTGAT
    Available Since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201152-plasmid-map-sequence-id-401799

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  3. pCDH-TRE3G-N-cG-CMV-P

    Plasmid
    #192886
    Purpose
    Tet inducible Nanoluc copGFP expression
    Depositor
    Kazuhiro Oka
    Insert
    Nanoluc-P2A-copGFP
    Use
    Lentiviral
    Available Since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    192886-plasmid-map-sequence-id-378891

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  4. pcDNA3 H2B-mIFP T2A Mpro (C145A)

    Plasmid
    #163080
    Purpose
    Expresses inactive Mpro mutant and a near-infrared fluorescent protein mIFP fused to H2B
    Depositor
    Xiaokun Shu
    Insert
    Inactive Mpro mutant C145A and H2B-mIFP
    Expression
    Mammalian
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    163080-plasmid-map-sequence-id-320827

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  5. pT7-V5-SBP-C1-HsRRP41

    Plasmid
    #64871
    Purpose
    expresses V5- and SBP-tagged RRp41 in mamalian cells
    Depositor
    Elisa Izaurralde
    Insert
    RRP41 (EXOSC4 Human)
    Tags
    V5 and SBP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    64871-plasmid-map-sequence-id-109466

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  6. pJAT43

    Plasmid
    #204299
    Purpose
    For introducing heat shock inducble B3 recombinase. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-EGFP
    Use
    CRISPR
    Promoter
    ie1
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204299-plasmid-map-sequence-id-406188

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  7. pDL025_HA-rb_IgG_HC_15F11

    Plasmid
    #193622
    Purpose
    HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.
    Depositor
    Jennifer DeLuca
    Article
    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:
    Insert
    Anti_HA_15F11 rabbit heavy chain
    Tags
    signal peptide
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193622-plasmid-map-sequence-id-446783

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  8. pDL026_HA-rb_IgG_LC_15F11

    Plasmid
    #193623
    Purpose
    HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.
    Depositor
    Jennifer DeLuca
    Article
    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:
    Insert
    Anti-HA_15F11 rabbit light chain
    Tags
    signal peptide
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193623-plasmid-map-sequence-id-380458

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  9. TRUPATH Triple Ga11

    Plasmid
    #196059
    Purpose
    Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha11-RLuc8 (GNA11 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196059-plasmid-map-sequence-id-386126

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  10. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

    Plasmid
    #172318
    Purpose
    CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    172318-plasmid-map-sequence-id-343646

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  13. LLP340 pEF1a-NLS-scFvGCN4-DNMT3a (mutant)

    Plasmid
    #100944
    Purpose
    Single chain variable fragment antibody GCN4 fused to DNMT3a catalytic mutant, binds SunTag, with 3xTy1 tag
    Depositor
    Ryan Lister
    Article
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    scFvGCN4, sfGFP, GB1, DNMT3a catalytic mutant (DNMT3A Human, Synthetic)
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    F636A, E660A, E725A, R295A
    Available Since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    100944-plasmid-map-sequence-id-206112

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  14. SunTagng14aa

    Plasmid
    #106439
    Purpose
    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.
    Depositor
    Steven Jacobsen
    Article
    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Insert
    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106439-plasmid-map-sequence-id-205526

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  15. TRUPATH Triple Ga15

    Plasmid
    #196060
    Purpose
    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196060-plasmid-map-sequence-id-385870

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  17. pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA)

    Plasmid
    #117168
    Purpose
    CRISPR Cas9 SunTag system to target NtDRMcd (without an NLS) to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    117168-plasmid-map-sequence-id-238529

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  24. TRUPATH Triple Gagust

    Plasmid
    #196054
    Purpose
    Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaGustducin-RLuc8 (GNAT3 Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196054-plasmid-map-sequence-id-393090

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  25. TRUPATH Triple GaoB

    Plasmid
    #196052
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196052-plasmid-map-sequence-id-387470

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  26. TRUPATH Triple GaZ

    Plasmid
    #196053
    Purpose
    Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaz-RLuc8 (GNAZ Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196053-plasmid-map-sequence-id-387469

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  27. pYPQ-ABE-Cas9n-Act3.0

    Plasmid
    #178956
    Purpose
    It consists of a Cas9 nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    ABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178956-plasmid-map-sequence-id-354328

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  28. pYPQ-CBE-SpRYn-Act3.0

    Plasmid
    #178958
    Purpose
    It consists of a SpRY nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2 -SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    hA3A-SpRY-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178958-plasmid-map-sequence-id-354326

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  29. pYPQ-dpcoCas9-Act3.0

    Plasmid
    #158408
    Purpose
    CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158408-plasmid-map-sequence-id-330919

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  30. pZH509

    Plasmid
    #102664
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102664-plasmid-map-sequence-id-199389

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  31. pYPQ-ABE-SpRYn-Act3.0

    Plasmid
    #178959
    Purpose
    It consists of a SpRY nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    ABE8e-SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178959-plasmid-map-sequence-id-354322

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  34. pJAT56

    Plasmid
    #204300
    Purpose
    Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-EGFP
    Use
    CRISPR
    Promoter
    3XP3
    Available Since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204300-plasmid-map-sequence-id-405836

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  39. pEG302-SunTag-FWAgRNA-4-22aa-TET1cd

    Plasmid
    #115344
    Purpose
    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter
    Depositor
    Steven Jacobsen
    Insert
    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    March 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    115344-plasmid-map-sequence-id-222905

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  40. SunTag-FWAgRNA-4-14aa-TET1cd

    Plasmid
    #106436
    Purpose
    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter
    Depositor
    Steven Jacobsen
    Article
    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Insert
    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS (TET1 Mustard Weed, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    106436-plasmid-map-sequence-id-267957

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  42. pSBL_DK186

    Plasmid
    #156422
    Purpose
    Mammalian expression of 3a-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells
    Depositor
    Stephen Brohawn
    Article
    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.
    Insert
    SARS-CoV-2 3a protein
    Tags
    EGFP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    156422-plasmid-map-sequence-id-306308

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  43. pM162

    Plasmid
    #173221
    Purpose
    Expresses ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP(1-9)-GB1 and Puro-T2A-MCP-mCherry-GFP11-GB1
    Depositor
    Hajin Kim
    Article
    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do
    Inserts
    ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP (1-10)-GB1
    MCP-mCherry-GFP11-GB1
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    173221-plasmid-map-sequence-id-341549

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  46. human ACF

    Plasmid
    #112860
    Purpose
    plasmid to overexpress human APOBEC1-complementation factor (ACF; A1CF) in mammalian cells
    Depositor
    Silvestro Conticello
    Article
    Chieca et al bioRxiv 2021
    Insert
    APOBEC1 complementation factor (A1CF Human)
    Expression
    Mammalian
    Available Since
    Oct. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    112860-plasmid-map-sequence-id-224730

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  49. pVP144

    Plasmid
    #222081
    Purpose
    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and eforRED for plasmid eviction.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al Biodes Res. 2025 Feb 27;7(1):100001. doi
    Insert
    dCas9-PmCDA1, sfGFP, sacB and eforRED
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    222081-plasmid-map-sequence-id-438346

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Showing: 6251 - 6300 of 6626 results