165,947 results

1. pAAVS1-Neo-CAG-M2rtTA-H2BmNeon (donorAB-mNeon)

   Plasmid

   #85799

   Purpose

   donorAB-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP

   Depositor

   Roland Friedel

   Article

   Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

   Insert

   PPP1R12C (PPP1R12C Human)
   

   Use

   Human targeting

   Mutation

   homology arms for knock-in into first intron

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Active Wnt8B-V5

   Plasmid

   #43819

   Depositor

   Xi He

   Article

   MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

   Insert

   WNT8B (WNT8B Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV0356

   Plasmid

   #133468

   Purpose

   Integration into fission yeast genome at ade6 locus

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. plentiCRISPR-sgGOT1

   Plasmid

   #72874

   Purpose

   The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

   Depositor

   David Sabatini

   Article

   Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

   Insert

   sgGOT1
   

   Use

   Lentiviral

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pB2AR-miRFP670nano3

   Plasmid

   #184671

   Purpose

   Beta-2-adrenergic receptor internally tagged with miRFP670nano3

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   B2AR-miRFP670nano3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRetroX-TRE3G-SopE

   Plasmid

   #221327

   Purpose

   Dox-inducible expression of SopE in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   SopE
   

   Use

   Retroviral

   Tags

   FLAG

   Promoter

   TRE3GV

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-ASNS-OE-IRES-Thy1.1

   Plasmid

   #192947

   Purpose

   Asparagine synthetase overexpression in murine cells

   Depositor

   Ping-Chih Ho

   Article

   Fernandez-Garcia et al Cell Rep. 2022 Nov 15;41(7):111639. doi:

   Insert

   Asparagine synthetase (Asns Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   5'LTR

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTn7-SCOUT22.RY

   Plasmid

   #201018

   Purpose

   pLv1-pUC18R6KT-miniTn7-Km-mCherry-sYFP2, KmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Inserts

   mcherry
   sYFP2
   

   Expression

   Bacterial

   Promoter

   J23104

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTn7-SCOUT22.YB

   Plasmid

   #201019

   Purpose

   pLv1-pUC18R6KT-miniTn7-Km-sYFP2-TagBFP, KmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Inserts

   sYFP2
   TagBFP
   

   Expression

   Bacterial

   Promoter

   J23104

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAV0413

    Plasmid

    #133470

    Purpose

    Integration into fission yeast genome at his5 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAV0357

    Plasmid

    #133469

    Purpose

    Integration into fission yeast genome at lys3 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5-HA-Ubiquitin-K33

    Plasmid

    #17607

    Purpose

    Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K33 only, other lysines mutated to arginines; P19…

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDRf1

    Plasmid

    #36027

    Depositor

    Wolf Frommer, Dominique Loque

    Article

    Loque et al Nature. 2007 Mar 8;446(7132):195-8. Epub

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. PET-B-HypaSpCas9-NLS-6xHis

    Plasmid

    #207386

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HypaSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A, amino acids 1005-1013…

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHage2-EF1a-mCherryTRF1-IRES-Blast

    Plasmid

    #182588

    Purpose

    expresses mcherry-trf1 in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

    Insert

    hTRF1 (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-15F11-HA-Halo

    Plasmid

    #129592

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. mPapaya1-H2B-6

    Plasmid

    #56651

    Purpose

    Localization: Nucleus/Histones, Excitation: 530, Emission: 541

    Depositor

    Michael Davidson

    Article

    Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

    Insert

    H2B (H2BC11 Human)
    

    Tags

    mPapaya1

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3-RARE-luciferase

    Plasmid

    #13458

    Depositor

    T. Michael Underhill

    Article

    Hoffman et al J Cell Biol. 2006 Jul 3. 174(1):101-13.

    Insert

    Retinoic Acid Receptor Response Element
    

    Use

    Luciferase

    Available Since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. GAL4UAS-Luciferase reporter

    Plasmid

    #64125

    Purpose

    Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    GAL4UAS
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    GAL4UAS

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223-NME6

    Plasmid

    #23562

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    NME6 (NME6 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pBSHSP:Cre;CMV:tdTomato-SceI

    Plasmid

    #30525

    Depositor

    Gerhart Ryffel

    Article

    Roose et al Transgenic Res. 2009 Aug . 18(4):595-605

    Insert

    Cre recombinase tdTomato
    

    Use

    Cre/Lox; Heat shock inducible, i-scei megabase ve…

    Expression

    Mammalian

    Available Since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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22. pDS170-enNTS1

    Plasmid

    #199152

    Purpose

    JZ73, bacterial expression construct expressing thermostabilised rat neurotensin receptor 1 (enNTS1)

    Depositor

    Daniel Scott

    Article

    Bumbak et al Cell Rep. 2023 Jan 31;42(1):112015. doi:

    Insert

    enNTS1 (Ntsr1 Rat)
    

    Tags

    10xHis tag, Avi tag, MBP, and muGFP

    Expression

    Bacterial

    Mutation

    V208M, C273S, C391S and C393S (over NTS1-B5)

    Promoter

    Lac/T5

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA-Wnt8A-V5

    Plasmid

    #35935

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt8A (Wnt8a Human)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Silent Mutation A (600) --> G

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLKO.1 shPIK3CD.v1 puro

    Plasmid

    #58706

    Purpose

    Lentiviral shRNA vector for knockdown of human PIK3CD

    Depositor

    Kevin Janes

    Article

    Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

    Insert

    shPIK3CD (PIK3CD Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pMXs GFP-LC3-RFP

    Plasmid

    #117413

    Purpose

    Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.

    Depositor

    Noboru Mizushima

    Article

    Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

    Plasmid

    #139989

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pDONR221_SLC14A2

    Plasmid

    #132265

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC14A2 (SLC14A2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 19, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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28. pBbS8c_ddCpf1_final

    Plasmid

    #212040

    Purpose

    Bacterial expression of the CRISPRi system for genome editing in E. coli

    Depositor

    Quanjiang Ji

    Article

    Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

    Insert

    ddCpf1
    

    Use

    CRISPR

    Promoter

    L-arabinose-inducible promoter PBAD

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. nlsSalicS1-NR

    Plasmid

    #232192

    Purpose

    Negative control for nlsSalicS1

    Depositor

    Alexander Jones

    Article

    Tang et al Science. 2025 Oct 9;390(6769):188-194. d

    Insert

    nlsSalicS1-NR
    

    Tags

    His-tag and Myc

    Expression

    Plant

    Promoter

    P16

    Available Since

    Oct. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. Ring2-FLAG-AID-mClover

    Plasmid

    #246226

    Purpose

    Expression of Ring2-FLAG-AID

    Depositor

    Hengbin Wang

    Insert

    Ring2 (RNF2 Human)
    

    Use

    CRISPR

    Tags

    Flag-AID-mClover

    Available Since

    Oct. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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