4,142 results
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Plasmid#154194PurposeLentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only
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pDGM6
Plasmid#110660PurposeAdeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.DepositorInsertAAV6 cap genes, AAV2 rep genes, adenovirus helper genes
UseAAVTagsExpressionMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP
Plasmid#13031PurposeMammalian expression vector for cloning your gene fused to EGFPDepositorInsertEnhanced Green Fluorescent Protein
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21 (ORF-EG)
Plasmid#46948PurposeInducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interestDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mCherry
Plasmid#176016PurposeMammalian expression of mCherry. Parton lab clone DFQDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTight-9-124-BclxL
Plasmid#60857Purpose2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoterDepositorInsertmiR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterpTightAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFGET19_Ulp1
Plasmid#64697PurposeH6-Ulp1 from pHYRS52 into pET-28b(+)DepositorInsertULP1 (ULP1 Budding Yeast, Saccharomyces cerevisiae)
UseTags6xHisExpressionBacterialMutationUlp1(403-621)PromoterT7Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) eGFP
Plasmid#129020PurposeExpresses eGFPDepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
35S:RUBY
Plasmid#160908PurposeRUBY under the control of CaMV 35S promoter/marker for transformationDepositorInsertRUBY and CaMV 35S promoter
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBBR1MCS-2
Plasmid#85168PurposeMobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsExpressionBacterialMutationPromoterConstitutive (derepressed P-lac)Available sinceNov. 8, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSIRV-NFAT-eGFP
Plasmid#118031PurposeFluorescent reporter for NFAT activationDepositorInserteGFP
UseRetroviralTagsExpressionMutationPromoterminimal promoter with NFAT binding siteAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
8xGTIIC-luciferase
Plasmid#34615PurposeYAP/TAZ-responsive synthetic promoter driving luciferase expressionDepositorInsertsynthetic TEAD luciferase reporter
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-WT
Plasmid#17608PurposeMammalian expression of HA tagged ubiquitinDepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-CMV-bArrestin2-TEV
Plasmid#107245PurposeVector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.DepositorInsertARRB2
UseTagsTEV(NIA), tobacco etch virus NIAExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K48
Plasmid#17605PurposeMammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to argininesDepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK48 only, other lysines mutated to arginines. Enh…PromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax
Plasmid#174820PurposeMammalian expression of SpCas9 PEmax prime editorDepositorInsertPEmax
UseTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVpuro-CMV-N-3xFLAG
Plasmid#123223PurposeN-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.DepositorTypeEmpty backboneUseLentiviral; Gateway destinationTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K63
Plasmid#17606PurposeMammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to argininesDepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK63 only, other lysines mutated to arginines. Enh…PromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
EZ-Tet-pLKO-Puro
Plasmid#85966PurposeTet/Dox inducible shRNA lentivirus with puromycin selectionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMutationPromoterH1 / TetOAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 hygro
Plasmid#24150Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNAAvailable sinceMarch 15, 2010AvailabilityAcademic Institutions and Nonprofits only