We narrowed to 12,055 results for: cel.2

1. LentiCRISPR v2-sgAMPK alpha2 clone3

   Plasmid

   #162124

   Purpose

   Lentiviral sgRNA plasmid targeting human AMPK alpha2

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgAMPK alpha2 (PRKAA2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEGFP Kind2 S159/666E

   Plasmid

   #105313

   Purpose

   To study altered downstream functions of phospholylation-site mimetic kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S159/666E

   Promoter

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP KInd2 S159E

   Plasmid

   #105311

   Purpose

   To study altered downstream functions of phospholylation-site mimetic kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S159E

   Promoter

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP Kind2 S159/666A

   Plasmid

   #105309

   Purpose

   To study altered downstream functions of phospholylation-site null kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S159/666A

   Promoter

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFP Kind2 S666A

   Plasmid

   #105308

   Purpose

   To study altered downstream functions of phospholylation-site null kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S666A

   Promoter

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFP KInd2 S159A

   Plasmid

   #105306

   Purpose

   To study altered downstream functions of phospholylation-site null kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S159A

   Promoter

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti-EF1a-dCas9-VPR-Puro

   Plasmid

   #99373

   Purpose

   3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   (Sp)dCas9-VPR-P2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TriEx-4 DHX36

   Plasmid

   #68368

   Purpose

   Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.

   Depositor

   James Vaughn

   Article

   Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-2

   Insert

   DHX36 (DHX36 Human)
   

   Use

   Tags

   Histidine

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV ie enhancer/promoter

   Available Since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pEUI(+)

   Plasmid

   #111834

   Purpose

   For mamalian expression of Ecdysone receptor-based inducible transgene expression switch.

   Depositor

   Hyunju Ro

   Article

   Lee et al Mol Ther Nucleic Acids. 2016 Sep 27;5(9)

   Insert

   GAL4-minimal VP16-EcR (EcR Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SV40, 10XUAS

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. CTCF-mAC donor (Hygro)

    Plasmid

    #140646

    Purpose

    CTCF tagging with mAID-clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CTCF (CTCF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP-C1-AR Q48

    Plasmid

    #86428

    Purpose

    Fluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine region

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    Androgen receptor (AR) (AR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Poly-glutamine expansion, G130D

    Promoter

    CMV

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept

    Plasmid

    #182424

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-Etanercept fusion proteins

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ-mNeongreen-Etanercept (TNFRSF1B Human)
    VNp-LZ
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp), and Vesicle Nuc…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-C1-AR Q0

    Plasmid

    #86427

    Purpose

    Fluorescent human androgen receptor (fused to EGFP) with deleted polyglutamine region

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    androgen receptor (AR) (AR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of the poly-glutamine expansion

    Promoter

    CMV

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-BRD7

    Plasmid

    #65379

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BRD7 (BRD7 Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Rb-379-928-pGex2TK

    Plasmid

    #21749

    Depositor

    William Kaelin

    Article

    Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion mutant ORF contains aa 379-928 of wt Rb …

    Promoter

    Available Since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMK262 (RAD21-mAC Neo)

    Plasmid

    #140538

    Purpose

    RAD21 tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    RAD21 (RAD21 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CTCF-mAC donor (Neo)

    Plasmid

    #140645

    Purpose

    CTCF tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CTCF (CTCF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX330-sgAAVS1

    Plasmid

    #85802

    Purpose

    Cas9/CRISPR vector for cut at human AAVS1 gene intron1

    Depositor

    Roland Friedel

    Article

    Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

    Insert

    PPP1R12C (PPP1R12C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti_CRISPRi_sgAR1

    Plasmid

    #167000

    Purpose

    Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    AR Promoter sgRNA 1 (AR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE N' EGFP NAP1 FL IRES Blast

    Plasmid

    #179100

    Purpose

    Expression of full length NAP1 cDNA

    Depositor

    Alicia Pickrell

    Article

    Paul et al J Cell Biol. 2024 Feb 5;223(2):e20230308

    Insert

    Nak associated protein 1 (AZI2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. RRL.sin.cPPT.SFFV/Ace2.WPRE (MT136)

    Plasmid

    #145842

    Purpose

    Lentiviral vector genome to ectopically express human Ace2

    Depositor

    Caroline Goujon

    Article

    Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

    Insert

    human Ace2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Lenti_CRISPRi_sgAR3

    Plasmid

    #167002

    Purpose

    Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    AR Promoter sgRNA 3 (AR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. DHC1 CRISPR

    Plasmid

    #140545

    Purpose

    DHC1 tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-EF1α-Flex/3'USS-hM4D/2A/GFP(ATG mut)

    Plasmid

    #197892

    Purpose

    Can be used to generate AAV virus that will express hM4D/2A/GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    hM4D/2A/GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    EF1a

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ACTB-(A)2x

    Plasmid

    #112056

    Purpose

    ACTB mRNA tagged with 2 copies of Riboglow (variant A)

    Depositor

    Amy Palmer

    Article

    Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

    Insert

    ACTB (ACTB Human)
    

    Use

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    2 copies of Riboglow tag (variant A) after stop c…

    Promoter

    CMV

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJC-elav1.8kb-TALE1-VP64-P10

    Plasmid

    #104609

    Purpose

    Expresses TALE1 under the control of a 1.8kb elav enhancer element

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    1.8kb elav enhancer element (elav Fly)
    

    Use

    TALEN

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available Since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HOXB6 (human) FLAG pMSCV

    Plasmid

    #8534

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB6 (HOXB6 Human)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    contains improved ribosomal binding site and FLAG…

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

28. pEGFP Kind2 S159/181/666A

    Plasmid

    #105310

    Purpose

    To study altered downstream functions of phospholylation-site null kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S159/181/666A

    Promoter

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEX Grap2-SH2

    Plasmid

    #46435

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Grap2 (GRAP2 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 52-159

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pU6-crRNA(TBK1)

    Plasmid

    #109322

    Purpose

    AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TBK1

    Depositor

    Feng Zhang

    Article

    Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

    Insert

    mCherry-KASH (TBK1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGEX-4T1_GST-GBRPL2

    Plasmid

    #223730

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    GABARAPL2 (GABARAPL2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 2-116 only, with Glycine exposed

    Promoter

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PGK_ACE2-ECD_Notch-Gal4VP64 (pZYW037)

    Plasmid

    #194226

    Purpose

    Lentiviral expression vector expressing ACE2-ECD SynNotch and mCherry transduction marker

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    PGK-ACE2-SynNotch-T2A-mCherry (ACE2 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMSCV-IRES-GFP/CALR ins5 YD/FL-FLAG

    Plasmid

    #214706

    Purpose

    Mammalian expression of human CALR ins5 YD/FL-FLAG

    Depositor

    Norio Komatsu

    Article

    Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

    Insert

    CALR ins5 YD/FL-FLAG (CALR Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    MSCV

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCDNA3-SREBP-1-GFP (A317-318; A341; A1171)

    Plasmid

    #204199

    Purpose

    Mammalian expression of SREBP-1 (isoform 1)

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SREBP-1 (SREBF1 Human)
    

    Use

    Tags

    3xHA, eGFP and T7x2

    Expression

    Mammalian

    Mutation

    Gly to Ala 317,318,341,1171

    Promoter

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV_SEPT9_i1 S22A S23A-msfGFP

    Plasmid

    #180338

    Purpose

    mammalian expression of human SEPT9_i1 S22A S23A fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 S22A S23A

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV_SEPT9_i1 R10A R15A-msfGFP

    Plasmid

    #180336

    Purpose

    mammalian expression of human SEPT9_i1 R10A R15A fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 R10A R15A

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV_SEPT9_i1 S12A S13A-msfGFP

    Plasmid

    #180337

    Purpose

    mammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 S12A S13A

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Lenti_CRISPRi_sgAR10

    Plasmid

    #167003

    Purpose

    Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    AR Promoter sgRNA 10 (AR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. DHC1-mIAA17 Hygro

    Plasmid

    #140544

    Purpose

    DHC1 tagging with mIAA7

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDNA5-LAP-MPS1-dTPR

    Plasmid

    #114048

    Purpose

    Expression of exogenous LAP tagged MPS1 delta TPR (TPR is deleted)

    Depositor

    Geert Kops

    Article

    Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

    Insert

    MPS1-dTPR (TTK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    delta TPR (deletion of TPR domain 63-191)

    Promoter

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCDNA5-LAP-MPS1-d60

    Plasmid

    #114046

    Purpose

    Expression of exogenous LAP tagged MPS1 delta 60 (NTE is deleted)

    Depositor

    Geert Kops

    Article

    Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

    Insert

    MPS1-d60 (TTK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    delta 60 (deletion of NTE domain)

    Promoter

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pEGFP Kind2 S159/181/666E

    Plasmid

    #105314

    Purpose

    To study altered downstream functions of phospholylation-site mimetic kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S159/181/666E

    Promoter

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pEVOL-pAzF[TAG]

    Plasmid

    #164579

    Purpose

    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Inserts

    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    araBAD, glnS, and proK

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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44. pEMS2181

    Plasmid

    #158421

    Purpose

    AAV plasmid with Ple32 (CLDN5 MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple32 (CLDN5 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple32

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. CRY2high-mCherry-Raf1

    Plasmid

    #104064

    Purpose

    Expresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry and Raf1

    Depositor

    Bianxiao Cui

    Article

    Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

    Insert

    CRY2high-mCherry-Raf1 (CRY2, RAF1 Mustard Weed, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    CRY2PHR E490R

    Promoter

    CMV

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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46. tetO-HAND2

    Plasmid

    #170690

    Purpose

    doxycycline-inducible overexpression of HAND2

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    HAND2 (HAND2 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. RRL.sin.cPPT.SFFV/Ace2.IRES-neo.WPRE (MT129)

    Plasmid

    #145840

    Purpose

    Lentiviral vector genome to ectopically express human Ace2

    Depositor

    Caroline Goujon

    Article

    Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

    Insert

    human Ace2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. tetO-NR2F1

    Plasmid

    #170698

    Purpose

    doxycycline-inducible overexpression of NR2F1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    nuclear receptor subfamily 2 group F member 1 (NR2F1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    deletion of 36G- please see depositor comments

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pSems-leader-mEGFP-MBP-TMD(ALA7)

    Plasmid

    #193051

    Purpose

    Expression of artificial transmembrane protein tagged with mEGFP and MBP at the plasma membrane. Control construct for single molecule co-localization and co-tracking microscopy.

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    ALA7-TMD
    

    Use

    Tags

    Ig k-chain leader sequence, MBP, and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pEMS2230

    Plasmid

    #158420

    Purpose

    AAV plasmid with Ple253 (PITX3 MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple253 (PITX3 )
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple253

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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