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pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE

Plasmid

#20944

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Publication

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

H134R

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
FU-tet-o-hKLF4

Plasmid

#19777

Depositor

Konrad Hochedlinger

Publication

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

Klf4 (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLKO.1 hygro shRNA E-cadherin

Plasmid

#18802

Depositor

Bob Weinberg

Publication

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

E-cadherin shRNA (CDH1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

July 22, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMevT

Plasmid

#17815

Depositor

Jay Keasling

Publication

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCD/NL-BH*DDD

Plasmid

#17531

Depositor

Jakob Reiser

Publication

Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

Insert

HIV-1 Gag/Pol, Tat, Rev

Use

Lentiviral

Expression

Mammalian

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pCS2 mt-GFP (BamHI minus)

Plasmid

#15681

Depositor

Michael Klymkowsky

Type

Empty backbone

Use

Xenopus/mammalian/avian/zebrafish

Tags

6x Myc and GFP

Available since

Sept. 14, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV5 Smad2-HA

Plasmid

#14930

Depositor

Joan Massague

Publication

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (SMAD2 Human)

Tags

HA

Expression

Mammalian

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
LV-Lac

Plasmid

#12108

Depositor

Inder Verma

Publication

Pfeifer et al Proc Natl Acad Sci U S A. 2001 Sep 25. 9

Insert

LacZ

Use

Lentiviral

Expression

Mammalian

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pEGFP-C2 RanGAP

Plasmid

#13378

Depositor

Mary Dasso

Publication

Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602

Insert

RanGAP (RANGAP1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLenti6-DEST PINK1-V5 KD

Plasmid

#13319

Depositor

Mark Cookson

Publication

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Kinase dead: K219A/D362A/D384A

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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RcCMV/Rb

Plasmid

#1763

Depositor

Bob Weinberg

Insert

human Rb (RB1 Human)

Expression

Mammalian

Promoter

CMV

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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1271 pBabe puroL Myr HA Akt2

Plasmid

#9018

Depositor

William Sellers

Insert

Akt2 (AKT2 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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PB-TO-ASCL1-DLX2

Plasmid

#182307

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Publication

van Voorst et al bioRxiv [Preprint]. 2025 Jul 7:2025.06.3

Inserts

Ascl1 (Ascl1 Mouse)
Dlx2 (Dlx2 Mouse)
puroR-T2A-mycNLS-mTagBFP2

Tags

None and T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and pTRE3G-bidirectional

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV mGAD65(delE1) GFP WPRE SV40pA

Plasmid

#177316

Purpose

To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.

Depositor

Hirokazu Hirai

Publication

Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10

Insert

mGAD65(delE1) promoter, GABAergic interneurons specific promoter

Use

AAV

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pRT043-CLYBL-DDdcas9-VPH

Plasmid

#158091

Purpose

Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

Depositor

Martin Kampmann

Publication

Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

Insert

CLYBL-CAG-DDdCas9VPH-T2A-GFP

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-FLEx-ER-TurboID

Plasmid

#160857

Purpose

Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Publication

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Has service

AAV8

Insert

ER TurboID

Use

AAV

Tags

HA and V5

Promoter

CAG

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hsyn-GRAB_5-HT1.0

Plasmid

#140552

Purpose

Expresses the 5-HT sensor GRAB_5-HT1.0 in neurons

Depositor

Yulong Li

Publication

Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s

Has service

AAV9

Insert

5-HT sensor GRAB_5-HT1.0

Use

AAV

Promoter

hSyn

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Cbh_v5 AAV-CBE N-terminal

Plasmid

#137175

Purpose

AAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Publication

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE N-terminal; U6-protospacer

Use

AAV

Mutation

Cas9 D10A

Promoter

Cbh

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pREP-8xARE-GFP-SV40-BFP

Plasmid

#134910

Purpose

NRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFP

Depositor

Markus Landthaler

Publication

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Inserts

8xARE- minimal promoter - GFP
TagBFP

Expression

Mammalian

Promoter

8xARE - minimal promoter and SV40

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CMV-mCherry-(F30-2xPepper)10

Plasmid

#129401

Purpose

mCherry mRNA reporter tagged with (F30-2xPepper)10

Depositor

Samie Jaffrey

Publication

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

mCherry-(F30-2xPepper)10

Expression

Mammalian

Promoter

CMV

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mNeonGreen-Giantin

Plasmid

#98880

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Giantin

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pAAV-CAG-NLS-GFP

Plasmid

#104061

Purpose

AAV expression of nuclear localized GFP with SV40 NLS

Depositor

Viviana Gradinaru

Publication

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has service

AAV PHP.eB

Insert

GFP

Use

AAV

Promoter

CAG

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pHIV-iRFP720-E2A-Luc

Plasmid

#104587

Purpose

Lentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoter

Depositor

Antonius Plagge

Publication

Comenge et al Elife. 2018 Jun 27;7. pii: 33140. doi: 1

Insert

iRFP720-E2A-Luciferase

Use

Lentiviral and Luciferase

Tags

bi-cistronic

Expression

Mammalian

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJL1-sfGFP

Plasmid

#102634

Purpose

In vitro expression of sfGFP from the T7 promoter

Depositor

Michael Jewett

Publication

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

sfGFP

Tags

strep tag

Expression

Bacterial

Promoter

T7

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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Lenti-entry-puro

Plasmid

#85745

Purpose

Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

Depositor

Qin Yan

Publication

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Type

Empty backbone

Use

Lentiviral

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV CD68-hM4D(Gi)-mCherry

Plasmid

#75033

Purpose

Expression of hM4D(Gi) using the CD68 promoter.

Depositor

Bryan Roth

Publication

Grace et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Has service

AAV1 and AAV9

Insert

hM4D(Gi)-mCherry

Use

AAV

Expression

Mammalian

Promoter

CD68

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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CIBN-CAAX

Plasmid

#79574

Purpose

expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting

Depositor

Pietro De Camilli, Olof Idevall-Hagren

Publication

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

CIBN (CIB1 Mustard Weed)

Tags

C-terminal CAAX-box

Expression

Mammalian

Mutation

K93A, R94A, K106A, K107A (please see depositor co…

Promoter

CMV

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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GFP-BLM

Plasmid

#80070

Purpose

Expresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)

Depositor

Nathan Ellis

Publication

Hu et al Hum Mol Genet. 2001 Jun 1;10(12):1287-98

Insert

BLM (BLM Human)

Tags

GFP

Expression

Mammalian

Mutation

If correct ID, the cDNA is from HeLa and has char…

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pDonor_mU6

Plasmid

#69350

Purpose

sgRNA scaffold and mouse U6 promoter

Depositor

Andrea Ventura

Publication

Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

Insert

mU6 promoter and sgRNA scaffold flanked by BbsI sites

Use

CRISPR

Promoter

mU6 promoter

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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phDLX2-N174

Plasmid

#60860

Purpose

2nd generation lentiviral transfer plasmid. Expresses DLX2 under the EF1a promoter

Depositor

Andrew Yoo

Publication

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

DLX2 (DLX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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