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178,651 results

Showing: 3901 - 3930 of 178651 results

  6. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid
    #107942
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R, and N282A
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107942-plasmid-map-sequence-id-259760

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  9. pcDNA3.1-hLbCpf1(TYCV) (pY230)

    Plasmid
    #89355
    Purpose
    Expresses humanized LbCpf1 variant that recognizes TYCV PAMs
    Depositor
    Feng Zhang
    Publication
    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cpf1, RR variant
    Use
    CRISPR
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    G532R/K595R
    Promoter
    CMV
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89355-plasmid-map-sequence-id-178226

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  13. CIBN-CAAX

    Plasmid
    #79574
    Purpose
    expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting
    Depositor
    Pietro De Camilli, Olof Idevall-Hagren
    Publication
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CIBN (CIB1 Mustard Weed)
    Tags
    C-terminal CAAX-box
    Expression
    Mammalian
    Mutation
    K93A, R94A, K106A, K107A (please see depositor co…
    Promoter
    CMV
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    79574-plasmid-map-sequence-id-314173

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  14. pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)

    Plasmid
    #79620
    Purpose
    Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells
    Depositor
    Akihiko Kondo
    Publication
    Nishida et al Science. 2016 Aug 4. pii: aaf8729.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9
    Tags
    PmCDA1 and UGI
    Expression
    Mammalian
    Mutation
    D10A for nickase Cas9
    Promoter
    pCMV
    Available since
    July 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    79620-plasmid-map-sequence-id-154120

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  15. GFP-BLM

    Plasmid
    #80070
    Purpose
    Expresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)
    Depositor
    Nathan Ellis
    Publication
    Hu et al Hum Mol Genet. 2001 Jun 1;10(12):1287-98
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BLM (BLM Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    If correct ID, the cDNA is from HeLa and has char…
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80070-plasmid-map-sequence-id-423157

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  17. pYPQ152

    Plasmid
    #69303
    Purpose
    Gateway entry vector with pco-dCas9-VP64
    Depositor
    Yiping Qi
    Publication
    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pco-dCas9-VP64 (Plant codon-optimized)
    Use
    CRISPR
    Mutation
    VP64 activator fusion; NLS fusion
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69303-plasmid-map-sequence-id-247582

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  21. pSBbi-Neo

    Plasmid
    #60525
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60525-plasmid-map-sequence-id-486715

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  22. pAC-BETA

    Plasmid
    #53272
    Purpose
    Contains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Publication
    Cunningham FX et al Plant Cell. 1996 Sep;8(9):1613-26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    crtE, crtY, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    53272-plasmid-map-sequence-id-487543

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Showing: 3901 - 3930 of 178651 results