We narrowed to 7,632 results for: ALP
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Plasmid#144329PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only
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hEBI3 pEF6-V5 (MJS_002)
Plasmid#72490PurposeExpression of human EBI3 in mammalian cells (V5 tag)DepositorAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mutant Human Cortactin-GFP
Plasmid#71365Purposemammalian expression of mutant human Cortactin-GFPDepositorInsertcortactin (CTTN Human)
TagsEGFPExpressionMammalianMutationsilent mutation present at 72nd nucleotidePromoterCMVAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro
Plasmid#110830PurposeExpresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.DepositorAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR4 mito roGFP2-Grx1
Plasmid#65000PurposeExpression of mitochondrial roGFP2-Grx1 under the alphaTub84B promoterDepositorInsertGlutaredoxin-1 (GLRX Human)
TagsroGFP2ExpressionInsectMutationmitochondrial targeting sequenceAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPuroR-MCS-LexA-VP16-MiniWhite
Plasmid#165898PurposePuromycin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloningDepositorInsertSelectable Empty Enhancer Driver
UseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROF141
Plasmid#155329PurposeBinary vector for expression of the PULSE system under the control of PAtUbi10 promoterDepositorInsertLB_PAtUbi10-SRDX-NLS-EL222-Tnos_SF_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB
UseSynthetic BiologyExpressionPlantAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C NLRP1 CARD WT
Plasmid#164008PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARDDepositorAvailable SinceFeb. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-APPL1-ΔPTB
Plasmid#59768PurposeExpresses APPL1 with PTB domain deletedDepositorInsertAPPL1 (APPL1 Human)
TagsEGFPExpressionMammalianMutationdeleted amino acids 467-709PromoterCMVAvailable SinceOct. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag
Plasmid#154970PurposeExpresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.DepositorInsertPfEBA175
TagsGFP and signal peptide (residues 1 to 32) from TG…ExpressionMammalianMutationinsert codes for EBA175 residues 1284 to 1462PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
R
Plasmid#167170PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cellsDepositorInsertsATP1A1-R
ATP1B1
ExpressionBacterial and InsectPromoterP10 and PHAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pONSY-CoNMM:mCherry
Plasmid#111878PurposeThis plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.DepositorInsertCapsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
UseCapsaspora owczarzakiTagsmCherryPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Elafin
Plasmid#18102DepositorInsertElafin (PI3 Human)
ExpressionMammalianAvailable SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEBG-IFNAR2(265-515)
Plasmid#12437DepositorInsertIFNAR2 a.a. 265-515 (IFNAR2 Human)
TagsGSTExpressionMammalianMutationpEBG vector is from Bruce Mayer, may be different…Available SinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cx43-Myc
Plasmid#60693Purposemammalian expression of Myc-tagged rat Cx43DepositorAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2z-tal1
Plasmid#164656Purposefor the synthesis of zebrafish tal1 mRNADepositorAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43
Plasmid#60692Purposeretroviral expression of rat Cx43DepositorAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 A66V
Plasmid#166817PurposeGateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)DepositorAvailable SinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
p1.1-Tr2-eGFP
Plasmid#162782PurposeFluorescent reporter for protein expression studiesDepositorInsertenhanced green fluorescent protein
ExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Myc-Idax WT
Plasmid#68553PurposeThis plasmid expresses full length IDAX.DepositorAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
HP1-ECFP-SNAP
Plasmid#187966Purposeexpresses HP1 with C-terminal tags ECFP and SNAPDepositorAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1458
Plasmid#143281PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pONSY-CoH2B:Venus
Plasmid#111877PurposeThis plasmid expresses Venus fluorescent protein fused to endogenous Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to transfect Capsaspora cells and visualize nucleus in vivo.DepositorInsertCapsaspora Histone H2B (CoH2B) fused to Venus
UseCapsaspora owczarzakiTagsVenusPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cx43-YA/VD
Plasmid#60699Purposemammalian expression of rat Cx43 YA/VD mutantDepositorAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 P1214R
Plasmid#166822PurposeGateway pDONR vector encoding NLRP1 with the patient mutation P1214R (Grandemange et al., 2017) (no stop codon)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pegAAT K342E correction
Plasmid#169841PurposeExpress a pegRNA used for correction (via A•T-to-G•C) of the E342K mutationDepositorInsertpegAAT K342E correction (SERPINA1 Human)
ExpressionMammalianAvailable SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43-YA/VD
Plasmid#60700Purposeretroviral expression of rat Cx43 YA/VD mutantDepositorInsertCx43 (Gja1 Rat)
UseRetroviralExpressionMammalianMutationYA/VD (Y286A/V289D)PromoterLTRAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
MBP-GTSE1(long)-His6
Plasmid#69110PurposeFor bacterial expression of recombinant human GTSE1 (longer isoform) tagged with MBP and His.DepositorAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPKm-245
Plasmid#90503PurposeExpresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - P2A - MTS - tFD - P2A - MTS - tFNRDepositorInsertmito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
UseLentiviralAvailable SinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-CERT PH domain
Plasmid#58725PurposeExpresses GFP-Tagged CERT PH domain in yeastDepositorInsertcollagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
TagsGFP and MycExpressionYeastPromoterPHO5Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-MCS-QF-MiniWhite
Plasmid#165906PurposeBlasticidin resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloningDepositorInsertSelectable Empty Enhancer Driver
UseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPKm-244
Plasmid#90502PurposeExpresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - IRES - MTS - tFD - P2A - MTS - tFNRDepositorInsertmito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
UseLentiviralAvailable SinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
mKikGR-Tubulin-6
Plasmid#57330PurposeLocalization: Microtubules, Excitation: 507 / 583, Emission: 517 / 593DepositorAvailable SinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRLCon1-3180
Plasmid#31445DepositorAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
ss-ED-mCherry-GPI
Plasmid#163680PurposeExprsses the GPI-anchored luminal domain of ST6GAL1 with a mCherry tag in mammalian cellsDepositorInsertThe luminal domain of ST6 beta-galactoside alpha-2,6-sialyltransferase 1 with N-terminally tagged signal sequences (ST6GAL1 Human)
TagsGPI anchor, mCherry, and signal sequencesExpressionMammalianMutationThis chimera contains the ecto-domain of ST. Its …Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
p1.2-GS
Plasmid#162770PurposeProtein expression in CHO cellsDepositorTypeEmpty backboneExpressionMammalianPromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT131_GalL_cCDA1-NGBE3
Plasmid#145095PurposeExpressing base editor cCDA1-NGBE3 in yeast cellsDepositorInsertcCDA1-NGBE3
UseCRISPRExpressionYeastMutationspCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
S
Plasmid#167172PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant in Sf9 cellsDepositorInsertsATP1A1-S
ATP1B1
ExpressionBacterial and InsectPromoterP10 and PHAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pG418R-MCS-QF-MiniWhite
Plasmid#165902PurposeG418 resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloningDepositorInsertSelectable Empty Enhancer Driver
UseSynthetic BiologyAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Tac-TC
Plasmid#162492PurposeExpresses partial length Tac (TMD and cytosolic tail) with GFP tag in mammalian cellsDepositorInsertpartial length Tac (TMD and cytosolic tail) (IL2RA Human)
TagsGFPExpressionMammalianMutationTac is in partial length with its TMD and cysotol…Available SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-IgL-C99-T4L-rTA
Plasmid#177840PurposeTango assayDepositorInsertC terminal 99 amino acid fragment of human Alzheimer Amyloid beta precursor Protein (APP Human)
ExpressionMammalianAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1-myc-BirA*-NRASG12V
Plasmid#122209PurposeExpresses myc-BirA* fused to NRASG12V in mammalian cellsDepositorInsertNRASG12v (NRAS Human)
Tagsmyc-BirA*ExpressionMammalianMutationchanged G12 to VPromoterCMVAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pDB.His.MBP.3C NLRP1 UPA-CARD WT
Plasmid#164003PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARDDepositorAvailable SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 M77T
Plasmid#166818PurposeGateway pDONR vector encoding NLRP1 with the patient mutation M77T (Zhong et al., 2016) (no stop codon)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 A54T
Plasmid#166815PurposeGateway pDONR vector encoding NLRP1 with the patient mutation A54T (Zhong et al., 2016) (no stop codon)DepositorAvailable SinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
exon L-firefly
Plasmid#81006Purposeluciferase based mini-gene to report splicing at exon 25 in Drosophila paraylticDepositorInsertparalytic (para Fly)
Tagsfirefly luciferaseExpressionInsectMutationspanning exon 24 to exon 26, termination codon in…PromoterP-AC5 (Actin 5C)Available SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
ADAP1
Plasmid#163905PurposeFull length mouse ADAP1 for purification from insect cellsDepositorAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_FRS2_IRS
Plasmid#109815PurposeProtein expression and purification of FRS2_IRSDepositorInsertFRS2_IRS (FRS2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only