163,606 results

1. mCherry-Vav2

   Plasmid

   #129623

   Purpose

   RhoGEF

   Depositor

   Dorus Gadella

   Article

   Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

   Insert

   Vav2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mCherry-GAL9

   Plasmid

   #166689

   Purpose

   For creating a stable mCherry-GAL9 cell line

   Depositor

   Alan Sabirsh

   Article

   Munson et al Commun Biol. 2021 Feb 16;4(1):211. doi:

   Insert

   LGALS9 (LGALS9 Human)
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PDG459 V3

   Plasmid

   #226958

   Purpose

   CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPB-cT3G-cERP2-NR5A1

   Plasmid

   #192915

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of NR5A1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   NR5A1 (NR5A1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PPRE-H2B-eGFP

   Plasmid

   #84393

   Purpose

   Fluorescent reporter for PPARgamma activity

   Depositor

   Thierry Fournier, Severine Degrelle

   Article

   Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

   Insert

   PPARG response element (PPARG Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   PPRE

   Available Since

   Jan. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SaABE8e

   Plasmid

   #138500

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSaCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

   Plasmid

   #131461

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   Trp53-V5, H3F3A-EGFP-AU1

   Expression

   Mammalian

   Mutation

   Pdgfra D842V

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEBB-SBP-HA

   Plasmid

   #36100

   Depositor

   Ezra Burstein

   Type

   Empty backbone

   Use

   Tags

   HA and SBP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP hsp70

   Plasmid

   #15215

   Depositor

   Lois Greene

   Article

   Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

   Insert

   hsp70 (HSPA1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBBR15.2-2H12.D11opt

    Plasmid

    #221185

    Purpose

    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter (Internal Lab ID: UJ11239)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen2.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLBH531_MBP-Cas14a1 expression

    Plasmid

    #112500

    Purpose

    Expresses MBP fused to Cas14a1

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. OaAEP1_C247A

    Plasmid

    #200307

    Purpose

    Bacterial expression plasmids for production of C247A mutant of asparaginyl endopeptidase 1 from O. affinis (OaAEP1_C247A)

    Depositor

    Artur Krężel

    Article

    Antonenko et al Bioconjug Chem. 2023 Mar 15;34(4):719-27

    Insert

    Asparaginyl endopeptidase from Oldenlandia affinis
    

    Use

    Tags

    Smt3

    Expression

    Bacterial

    Mutation

    Cysteine 247 to Alanine

    Promoter

    T7

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL4.23-GW

    Plasmid

    #60323

    Purpose

    This is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-6p-2-EWSR1

    Plasmid

    #46384

    Depositor

    Heinz Gehring

    Article

    Pahlich et al Proteins. 2005 Oct 1;61(1):164-75.

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-EF1α-DIO-CFL-SN

    Plasmid

    #181740

    Purpose

    AAV vector for expressing CFL-SN

    Depositor

    Yasunori Hayashi

    Article

    Goto et al Science. 2021 Nov 12;374(6569):857-863.

    Insert

    CFL-SN
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHBintE

    Plasmid

    #48136

    Purpose

    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TFORF2494

    Plasmid

    #142112

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZBTB18 (ZBTB18 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT3TS-spRYCas9-ABE-UMax

    Plasmid

    #222139

    Purpose

    Near PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    TadA-nSpRYCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FRB-mCherry-Giantin

    Plasmid

    #186575

    Purpose

    Expression of FRB-mCherry on Golgi membrane with Giantin transmembrane (residues 3131-3259)

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    Giantin (GOLGB1 Human)
    

    Use

    Tags

    FRB-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNatA (pACYCduet-naa10-naa15)

    Plasmid

    #72928

    Purpose

    Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

    Inserts

    naa10 (naa10 Fission Yeast, Synthetic)
    naa15 (naa15 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optomised for E.coli expression

    Promoter

    T7

    Available Since

    Feb. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PMP34-mCherry-EGFP-Tet-On

    Plasmid

    #109017

    Purpose

    Tet-On construct to induce expression of tandem mCherry-eGFP tagged PMP34 (peroxisome marker)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    PMP34 (SLC25A17 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. BPK2014-AaCas12b

    Plasmid

    #121949

    Purpose

    E. coli expression, protein purification

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b
    

    Use

    Tags

    10xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TFORF1906

    Plasmid

    #141883

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF7L2 (TCF7L2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPROBE-GT

    Plasmid

    #37814

    Depositor

    Steven Lindow

    Article

    Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    Promotorless gfp reporter gene
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pUCmini-iCAP-PHP.B

    Plasmid

    #103002

    Purpose

    non-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mTFP1-N1

    Plasmid

    #54521

    Purpose

    Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson, Robert Campbell

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Type

    Empty backbone

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTN4

    Plasmid

    #174212

    Purpose

    Donor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locus

    Depositor

    Masato Kanemaki

    Article

    Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

    Inserts

    AtTIR1(F79G)::mRuby (TIR1 Nematode)
    GFP
    

    Use

    CRISPR and Cre/Lox

    Tags

    mRuby

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. coel::RFP

    Plasmid

    #8938

    Depositor

    Piali Sengupta

    Article

    Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

    Insert

    unc-122 promoter (unc-122 Nematode)
    

    Use

    Tags

    RFP

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Nov. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pF1-Hs Cdk1/cyclin B1

    Plasmid

    #177330

    Purpose

    Co-expression of the human active Cdk1/cyclin B1 kinase complex

    Depositor

    Andreas Boland

    Article

    Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

    Inserts

    Cyclin-dependent kinase 1 (CDK1 Human)
    G2/mitotic-specific cyclin-B1 (CCNB1 Human)
    

    Use

    Tags

    2 x Strep-tag and 8 x His

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. RNASEL gRNA (BRDN0001149212)

    Plasmid

    #77051

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge