159,258 results

1. pTRIP-SFFV-mtagBFP-2A RIGI WT

   Plasmid

   #167289

   Purpose

   Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   DDX58 (RIGI Human)
   

   Use

   Lentiviral

   Tags

   tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AiP11633 - pAAV-hsA2-AiE0140h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1633)

   Plasmid

   #163503

   Purpose

   Direct-expressing SYFP2 AAV Virus

   Depositor

   The Allen Institute for Brain Science, Boaz Levi

   Article

   Mich et al Cell Rep. 2021 Mar 30;34(13):108754. doi

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   MinRho

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-C-mScarlet-Zeo

   Plasmid

   #207733

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. mCherry-Lifeact-7

   Plasmid

   #54491

   Purpose

   Localization: Action, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mCherry-Lifeact

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-MAP1B

   Plasmid

   #44396

   Depositor

   Phillip Gordon-Weeks

   Article

   Scales et al J Cell Sci. 2009 Jul 15;122(Pt 14):2424-

   Insert

   MAP1B (Map1b Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

   Plasmid

   #83841

   Purpose

   Fluorescent probe for G1/S transition and M/G1 transition

   Depositor

   Michael Lin

   Article

   Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-PinkyCaMP

   Plasmid

   #232856

   Purpose

   An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under hSyn promoter

   Depositor

   Olivia Masseck

   Article

   Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

   Insert

   PinkyCaMP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX551

   Plasmid

   #60957

   Purpose

   pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.

   Depositor

   Feng Zhang

   Article

   Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

   Insert

   SpCas9
   

   Use

   AAV and CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   pMecp2

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. eSrtA(2A-9) pET29b

   Plasmid

   #75145

   Purpose

   reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity

   Depositor

   David Liu

   Article

   Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

   Insert

   reprogrammed sortase A variant
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   S102C A104H E105D K138P K152I N160K K162H T164N K…

   Promoter

   T7

   Available since

   July 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

    Plasmid

    #171019

    Purpose

    Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    LargeBit-SNAPtag- Beta-actin
    

    Use

    Lentiviral and Luciferase

    Tags

    Beta actin and LargeBiT

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHO4d-Cas9

    Plasmid

    #67881

    Purpose

    Cas9nlsHis6 Bacterial Expression construct

    Depositor

    Michael Nonet

    Article

    Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and T7 epitope

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. ampl43

    Plasmid

    #161830

    Purpose

    pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

    Depositor

    Saeed Tavazoie

    Article

    Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_3xFlagEzh2 WT

    Plasmid

    #173717

    Purpose

    WT EZH2 expression plasmid with 3XFLAG tag

    Depositor

    Thomas Cech

    Article

    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

    Plasmid

    #226105

    Purpose

    ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    atf4 (ATF4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains uORF1-2 of ATF4, whose stability can ser…

    Promoter

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ecoli cpGFP

    Plasmid

    #215713

    Purpose

    Expresses cpGFP in Ecoli

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    cpGFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    circularly permuted GFP

    Promoter

    T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET9d-hnRNP-A1

    Plasmid

    #23026

    Depositor

    Douglas Black

    Insert

    hnRNPA1 (HNRNPA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPR

    Plasmid

    #42875

    Purpose

    A crRNA expression plasmid for targeting a specific sequence.

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    CRISPR-BsaI
    

    Use

    CRISPR; E.coli

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. YPet-N1

    Plasmid

    #54637

    Purpose

    Localization: N1 Cloning Vector, Excitation: 513, Emission: 527

    Depositor

    Patrick Daugherty, Michael Davidson

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

    Type

    Empty backbone

    Use

    Tags

    YPet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCS2+mNeonGreen-C Cloning Vector

    Plasmid

    #128144

    Purpose

    To express a protein of interest fused to the C-terminus of mNeonGreen

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    mNeonGreen fusion

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAIDA1

    Plasmid

    #79180

    Purpose

    A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags

    Depositor

    Gen Larsson

    Article

    Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi

    Insert

    Adhesin Involved in Diffuse Adherence
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PH-PLCD1-GFP

    Plasmid

    #51407

    Purpose

    Is a biosensor for PI(4,5)P2

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    PH-PLCD1 (aa 1–170) (PLCD1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKb-TnpB2

    Plasmid

    #215334

    Purpose

    ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site

    Depositor

    Kutubuddin Molla

    Article

    Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10

    Insert

    Rice codon optimized ISDra2 TnpB
    

    Use

    Tags

    Not applicable

    Expression

    Plant

    Mutation

    Promoter

    Rice ubiquitin promoter for TnpB and maize ubiqui…

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mCherry-Peroxisomes-2

    Plasmid

    #54520

    Purpose

    Localization: Peroxisomes, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    Peroximal Targeting Signal 1 (PTS1) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti CMV Blast DEST (706-1)

    Plasmid

    #17451

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Blast 

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMo130-TelR

    Plasmid

    #50799

    Purpose

    Suicide vector for generating chromosomal deletions in multidrug-resistant bacteria

    Depositor

    Kim Lee Chua

    Article

    Amin et al BMC Microbiol. 2013 Jul 13;13:158. doi:

    Insert

    tellurite-resistance cassette
    

    Use

    Suicide vector

    Tags

    Expression

    Mutation

    Promoter

    PCs12

    Available since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pX330A-1x2

    Plasmid

    #58766

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET47b-T7-enAsCas12f

    Plasmid

    #204642

    Purpose

    Expression of enAsCas12f in E. coli

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    enAsCas12f
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D196K, N199K, G276R, N328G, D364R

    Promoter

    T7

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

    Plasmid

    #159816

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDV044

    Plasmid

    #191824

    Purpose

    Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. pCPC600 promoter. With terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpR

    Depositor

    Jim Roberts

    Article

    Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pmScarlet-i_H2A_C1

    Plasmid

    #85053

    Purpose

    In vivo visualization of the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    H2A (H2AC16 Human)
    

    Use

    Tags

    mScarlet-i

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge