We narrowed to 11,551 results for: NSI

1. raACR-mCerulean3-pAAV2-hSyn

   Plasmid

   #204966

   Purpose

   Panneuronal expression of the MsACR1 mutant raACR fused to mCerulean3

   Depositor

   Peter Hegemann

   Article

   Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

   Insert

   raACR
   

   Use

   AAV

   Tags

   mCerulean3

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST

   Plasmid

   #105448

   Purpose

   Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

   Has Service

   AAV9

   Insert

   ChrimsonR-mRuby2-ST
   

   Use

   AAV

   Tags

   mRuby2 and soma targeting motif

   Expression

   Mutation

   K176R in Chrimson

   Promoter

   hSyn

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter)

   Plasmid

   #128607

   Purpose

   Expresses 36xMashTag with MoonTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Insert

   36xMashTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pcmv

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Venus-rab7a

   Plasmid

   #234743

   Purpose

   Mammalian expression of late endosome marker Venus-rab7a

   Depositor

   Nevin Lambert

   Article

   Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

   Insert

   Venus-rab7a (Rab7 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pARF6(Q67L)-CFP

   Plasmid

   #11387

   Depositor

   Joel Swanson

   Article

   Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

   Insert

   ADP Ribosylation Factor 6 (ARF6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACE-GIRK4-mC-S

   Plasmid

   #172427

   Purpose

   Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

   Depositor

   Arthur Laganowsky

   Article

   Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

   Insert

   GIRK4 (KCNJ5 Human)
   

   Use

   Tags

   mCherry Streptag II

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHAGE-IRES-puro-NLS-NLS-dMisCas13b-3xGFP-NLS

   Plasmid

   #191367

   Purpose

   Overexpression of NLS-NLS-dMisCas13b-3xGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-NLS-dMisCas13b-3xGFP-NLS
   

   Use

   Tags

   3xGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET_RGG

   Plasmid

   #124929

   Purpose

   Expression of RGG domain from LAF-1 gene

   Depositor

   Matthew Good, Daniel Hammer, Benjamin Schuster

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   LAF-1 RGG domain (laf-1 Nematode)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   RGG domain

   Promoter

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. GRK2-K220R

   Plasmid

   #35403

   Depositor

   Robert Lefkowitz

   Article

   Freedman et al J Biol Chem. 1995 Jul 28;270(30):17953-6

   Insert

   GRK2 (GRK2 Bovine)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K220R

   Promoter

   CMV

   Available Since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSMALB

    Plasmid

    #161786

    Purpose

    Gateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and TagBFP.

    Depositor

    John Dick, Peter van Galen

    Article

    van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Bidirectional minhCMV and SFFV

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-29b-3xGluGlu-c-jun-His6

    Plasmid

    #129227

    Purpose

    Encodes 3xGluGlu tagged human c-Jun for purification by nickel affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    cJun (JUN Human)
    

    Use

    Tags

    6xHistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYFJ16-LplA(W37V) (for E. Coli expression)

    Plasmid

    #34838

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LplA W37V (lplA E. Coli)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Mutated Trp37 to Val

    Promoter

    T5 promoter

    Available Since

    May 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV7.1_3xFLAG-WWC2

    Plasmid

    #172995

    Purpose

    constitutive expression of FLAG-tagged WWC2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-WWC2 (WWC2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS416Gal TDP43 WT YFP

    Plasmid

    #27447

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

15. EK0379 CMV-TO-EGFP-stop-t70587 (PB)

    Plasmid

    #191154

    Purpose

    Expression of Trigger 1 in the 3' UTR of EGFP in mammalian cells; used to make a cell line to study sensors for integrated 3' UTR or CDS triggers; expression is inducible with doxycycline due to CMV-TO promoter.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    EGFP-t70587
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG sHRPb-FKBP-post mGRASP

    Plasmid

    #73146

    Purpose

    Small fragment of split HRP and FKBP fused to the extracellular terminus of the "post mGRASP" scaffold published by Magee, in which the majority of the NLG1 extracellular domain is deleted.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    sHRPb-FKBP-post mGRASP
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTK23

    Plasmid

    #37405

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

    Insert

    Neuromodulin (GAP43 Human)
    

    Use

    Tags

    S-tag, TEV, and mCherry

    Expression

    Mammalian

    Mutation

    Contains the first 20aa of GAP43 (neuromodulin)

    Promoter

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCB168

    Plasmid

    #37389

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

    Insert

    dynein cytoplasmic 1 intermediate chain 1 (DYNC1I1 Human)
    

    Use

    Retroviral

    Tags

    GFP, S-tag, and TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR-ARF19

    Plasmid

    #83846

    Purpose

    Gateway donor vector for ARF19

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    ARF19 (ARF19 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJW6.01

    Plasmid

    #62882

    Purpose

    Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    GFP::MEG-3
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    meg-3

    Available Since

    March 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

    Plasmid

    #63168

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-V5"
    

    Use

    Tags

    N-myristoylation

    Expression

    Insect

    Mutation

    Promoter

    GAL4-responsive UAS hsp70

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKD184

    Plasmid

    #90953

    Purpose

    Expresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutively

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Inserts

    Thiosulfate response regulator
    Superfolder GFP
    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Bba_J23114, PltetO-1, and PphsA

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHES830

    Plasmid

    #87944

    Purpose

    Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    Zif268 DBD - hPR LBD - MSN2 AD (PGR Budding Yeast, Synthetic, Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 (crippled)

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMSCV-human-pro-IL-18

    Plasmid

    #214317

    Purpose

    Expression of gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myc

    Expression

    Mutation

    Promoter

    MESV

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MSCV-miRE_shBRD9_561-SV40-GFP

    Plasmid

    #75139

    Purpose

    Retroviral expression plasmid encoding shBRD9_561 (targeting human BRD)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBRD9_561
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pLP_mod_mNeonGreen11_P2A_puroR_CRE-GFP

    Plasmid

    #229227

    Purpose

    Modular BxB1 landing pad vector containing C-term mNeonGreen11 tag, puroR marker, and a cAMP GFP reporter.

    Depositor

    Willow Coyote-Maestas

    Article

    Howard et al Cell. 2025 Feb 6;188(3):671-687.e20. doi

    Type

    Empty backbone

    Use

    Tags

    mNeonGreen11

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pENTR-PKAKTRClover

    Plasmid

    #59146

    Purpose

    ORF encoding PKA KTR mClover flanked by gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    PKA Kinase Translocation Reporter (PRKACA Human, Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-TRE-DIO-hM3Dq-mCherry (Cre-OFF)

    Plasmid

    #166609

    Purpose

    Encodes Cre-inactivated hM3Dq-mCherry under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    hM3Dq-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mt deltaN20 TFB2MdeltaC3

    Plasmid

    #205062

    Purpose

    TFB2M protein expression

    Depositor

    Smita Patel

    Article

    Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

    Insert

    TFB2M (TFB2M Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks N-terminal 20 aa and C-terminal 3 aa

    Promoter

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-blueCKAR

    Plasmid

    #118472

    Purpose

    cpBFP-based single-color biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueCKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHAGE-NLS-dMisCas13b-SNAPf-NLS-3xFlag

    Plasmid

    #191536

    Purpose

    Overexpression of NLS-dMisCas13b-SNAPf-NLS-3xFlag in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-dMisCas13b-SNAPf-NLS-3xFlag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHAGE-IRES-puro-NLS-dMisCas13b-EGFP-NLS

    Plasmid

    #191359

    Purpose

    Overexpression of NLS-dMisCas13b-EGFP-NLS in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-dMisCas13b-EGFP-NLS
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHR-FKBP:mCherry-Rab7a

    Plasmid

    #72903

    Purpose

    Late endosome-targeted mCherry-tagged recruiter construct

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    Rab7a (RAB7A Human)
    

    Use

    Lentiviral

    Tags

    FKBP, mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDT-sgRNA-XMAS-1x

    Plasmid

    #164413

    Purpose

    Dual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHR-FKBP:mCherry-Rab11a

    Plasmid

    #72902

    Purpose

    Recycling endosome-targeted mCherry-tagged recruiter construct

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    Rab11a (RAB11A Human)
    

    Use

    Lentiviral

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    fragment a.a. 2-216

    Promoter

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET_RGG-RGG-RGG

    Plasmid

    #124938

    Purpose

    Expression of RGG-RGG-RGG

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFnCas12a_NT

    Plasmid

    #169838

    Purpose

    Expresses FnCas12a and guide RNA in Bacteria

    Depositor

    John van der Oost

    Article

    Mohanraju et al CRISPR J. 2021 Feb;4(1):82-91. doi: 10.1

    Insert

    FnCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pXynA

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pIBA3-SpyCatcher-sfGFP

    Plasmid

    #107420

    Purpose

    For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

    Depositor

    Jack Leo

    Article

    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

    Insert

    SpyCatcher
    

    Use

    Tags

    His tag, StrepII, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHH0103_TEC-1/1

    Plasmid

    #91252

    Purpose

    Protein expression and purification of human SH3 domain construct TEC-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    TEC-1/1 (TEC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. mCherry-Incenp wt

    Plasmid

    #108487

    Purpose

    expression of vsv-INCENP wt-mCherry

    Depositor

    Susanne Lens

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    INCENP (INCENP Human)
    

    Use

    Tags

    VSV and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge