We narrowed to 5,601 results for: Feng Zhang

1. pLenti_P2A_Puro

   Plasmid

   #211364

   Purpose

   Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

   Depositor

   Jacques Neefjes

   Article

   Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pErCas12a EZ Clone

   Plasmid

   #132641

   Purpose

   Expresses ErCas12a and an sgRNA to target a region of interest

   Depositor

   Stephen Ekker

   Article

   Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

   Insert

   ErCas12a
   

   Use

   AAV and CRISPR

   Tags

   3x HA and SV40 NLS

   Expression

   Mammalian

   Mutation

   Q926R (Please see depositor comments) and BsaI si…

   Promoter

   CMV Promoter

   Available since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lenti-SpCas9 blast

   Plasmid

   #104997

   Purpose

   This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. sgTP53(A)

   Plasmid

   #85559

   Purpose

   Expresses sgRNA targeting human TP53 around codon F109

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   tumor protein p53 (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

5. sgTP53(B)

   Plasmid

   #85560

   Purpose

   Expresses sgRNA targeting human TP53 around codon F109

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   tumor protein p53 (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

6. sgALK(A)

   Plasmid

   #85562

   Purpose

   Expresses sgRNA targeting human ALK around codon F1174

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   anaplastic lymphoma receptor tyrosine kinase (ALK Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

7. sgEGFR(A)

   Plasmid

   #85564

   Purpose

   Expresses sgRNA targeting human EGFR around codon T790

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   epidermal growth factor receptor (EGFR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

8. NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

   Plasmid

   #75236

   Purpose

   CRISPR/Cas9 plasmid against human NFATc1

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc1 (NFATC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. sgTP53(R273)

   Plasmid

   #85561

   Purpose

   Expresses sgRNA targeting human TP53 around codon R273

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   tumor protein p53 (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

10. NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75237

    Purpose

    CRISPR/Cas9 plasmid against human NFATc1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc1 (NFATC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. sgALK(B)

    Plasmid

    #85563

    Purpose

    Expresses sgRNA targeting human ALK around codon F1174

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    anaplastic lymphoma receptor tyrosine kinase (ALK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

12. Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75244

    Purpose

    CRISPR/Cas9 plasmid against human Helios

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Helios (IKZF2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. lentiCRISPR v2 kif26b sgRNA1

    Plasmid

    #102857

    Purpose

    Expresses sgRNA targeting mouse Kif26b

    Depositor

    Henry Ho

    Article

    Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

    Insert

    Mouse Kif26b (Kif26b Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. lentiCRISPR v2 kif26b sgRNA2

    Plasmid

    #102858

    Purpose

    Expresses sgRNA targeting mouse Kif26b

    Depositor

    Henry Ho

    Article

    Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

    Insert

    Mouse Kif26b (Kif26b Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. sgALK(Chr. Inv.)

    Plasmid

    #85569

    Purpose

    Expresses sgRNA targeting human ALK between exon 20 and 21

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    anaplastic lymphoma receptor tyrosine kinase (ALK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

16. sgEGFR(B)

    Plasmid

    #85565

    Purpose

    Expresses sgRNA targeting human EGFR around codon T790

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    epidermal growth factor receptor (EGFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

17. NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75238

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc1 (NFATC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75239

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc1 (NFATC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #223323

    Purpose

    Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

    Depositor

    Jonathan Arias

    Article

    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

    Insert

    SpCas9 and sgRNA-T2 (cas9 )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX335_U6-Chimeric_BB-CBh-hSpCas9n(D10A)_SOX17_Ex2_KI

    Plasmid

    #195502

    Purpose

    Cas9 nickase expression vector bearing a sgRNA targeting Exon 2 of human SOX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA (SOX17 Human)
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Neurog2 x2)

    Plasmid

    #171100

    Purpose

    CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Neurog2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9 (Neurog2 Mouse, S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Ascl1 x2)

    Plasmid

    #171099

    Purpose

    CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9 (Ascl1 Mouse, S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. lenti-sgRNA blast

    Plasmid

    #104993

    Purpose

    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. lenti-sgRNA puro

    Plasmid

    #104990

    Purpose

    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. lenti-sgRNA hygro

    Plasmid

    #104991

    Purpose

    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. lenti-sgRNA neo

    Plasmid

    #104992

    Purpose

    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCROPseq-VEX-optimized-scaffold

    Plasmid

    #203321

    Purpose

    Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. px330-Axin1

    Plasmid

    #162543

    Purpose

    sgRNA targeting Axin1

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Axin1 (Axin1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75240

    Purpose

    CRISPR/Cas9 plasmid against human Ikaros

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Ikaros-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75241

    Purpose

    CRISPR/Cas9 plasmid against human Ikaros

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid

    #163643

    Purpose

    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    

    Use

    Lentiviral

    Tags

    Luc2-P2A-tdTomato-T2A

    Expression

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    EFS and pU6

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AOI-WT-Cas9-sg-mouse Ezh2-E10-GFP

    Plasmid

    #91879

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Ezh2 (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

33. AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP

    Plasmid

    #91880

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Ezh2 (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

34. sgAPC(B)

    Plasmid

    #85558

    Purpose

    Expresses sgRNA targeting human APC around codon I1417

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    APC, WNT signaling pathway regulator (APC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

35. Ikaros-CRISPR-Nick2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75243

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human Ikaros (2/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75242

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human Ikaros (1/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PX459-CTNNA1(α-catenin CRISPR KO)

    Plasmid

    #229708

    Purpose

    Knockout of a-catenin in mammalian cells using CRISPR-Cas9 technology

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. px330-Kmt2b-4

    Plasmid

    #162538

    Purpose

    sgRNA targeting Kmt2b

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Kmt2b (Kmt2b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. px330-Kmt2c-1

    Plasmid

    #162531

    Purpose

    sgRNA targeting Kmt2c

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Kmt2c (Kmt2c Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. px330-Kmt2c-2

    Plasmid

    #162532

    Purpose

    sgRNA targeting Kmt2c

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Kmt2c (Kmt2c Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge