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SpyCatcher003 S49C

Plasmid

#133448

Purpose

Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

Depositor

Mark Howarth

Publication

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003 S49C

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Contains S49C mutation

Promoter

T7

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCS2+mNeonGreen-C Cloning Vector

Plasmid

#128144

Purpose

To express a protein of interest fused to the C-terminus of mNeonGreen

Depositor

Ken-Ichi Takemaru

Type

Empty backbone

Tags

mNeonGreen fusion

Expression

Mammalian

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pHAGE-KRAS-G12D

Plasmid

#116423

Purpose

Lentiviral expression of KRAS G12D

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

G12D

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET259-pUC57 24xMS2V6

Plasmid

#104391

Purpose

MS2V6 cloning

Depositor

Robert Singer, Evelina Tutucci

Publication

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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p11d-tRPA(123)

Plasmid

#102613

Purpose

Expression of human RPA in E. coli

Depositor

Marc Wold

Publication

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Inserts

RPA1 (RPA1 Human)
RPA2 (RPA2 Human)
RPA3 (RPA3 Human)

Expression

Bacterial

Promoter

T7

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pAAV-FLEX-EGFPL10a

Plasmid

#98747

Purpose

Cre-dependently expresses the fusion protein EGFPL10a

Depositor

Nathaniel Heintz, Alexander Nectow, Eric Schmidt

Publication

Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

Has service

AAV5 and AAV Retrograde

Insert

EGFPL10a

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Promoter

EF1a

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pCDNA3.1-FLAG-hSF3B1-K700E

Plasmid

#82577

Purpose

Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant)

Depositor

Manoj Pillai

Publication

Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

Insert

human SF3B1-K700E (SF3B1 Human)

Tags

FLAG peptide

Expression

Mammalian

Mutation

K700E

Promoter

CMV

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pBA439

Plasmid

#85967

Purpose

Perturb-seq vector backbone

Depositor

Jonathan Weissman

Publication

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAAV-mDlx-GCaMP6f-Fishell-2

Plasmid

#83899

Purpose

GCaMP6f expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Publication

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has service

AAV1, AAV9, and AAV Retrograde

Insert

GCaMP6f

Use

AAV

Expression

Mammalian

Promoter

mDlx

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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IMS-APEX2

Plasmid

#79058

Purpose

Expresses APEX in the mitochondrial intermembrane space.

Depositor

Alice Ting

Publication

Hung et al Mol Cell. 2014 Jul 17;55(2):332-41. doi:

Insert

APEX

Tags

V5

Expression

Bacterial and Mammalian

Promoter

T7 promoter

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCDH-EF1

Plasmid

#72266

Purpose

Express gene of interest under EF-1 promoter

Depositor

Kazuhiro Oka

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 11, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

20 Articles

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pCS6-SMASh-YFP

Plasmid

#68852

Purpose

Expresses SMASh tag fused to YFP at N terminus in mammalian cells

Depositor

Michael Lin

Publication

Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

Insert

SMASh-YFP

Tags

SMASh tag

Expression

Mammalian

Promoter

CMV

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pComb3XTT

Plasmid

#63891

Purpose

Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

Depositor

Carlos Barbas

Publication

Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

Insert

TT Fab

Use

Phage display

Tags

6xHis, HA tag, and geneIII

Expression

Bacterial

Promoter

lacZ

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCDH-puro-Bcl-XL

Plasmid

#46972

Purpose

lentiviral expression of Bcl-XL

Depositor

Jialiang Wang

Publication

Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCI-EGFP-NR2a wt

Plasmid

#45445

Depositor

Andres Barria, Robert Malinow

Publication

Barria et al Neuron. 2002 Jul 18;35(2):345-53.

Insert

NR2A (Grin2a Rat)

Tags

EGFP

Expression

Mammalian

Mutation

EGFP inserted after the predicted signal peptide …

Promoter

CMV

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA

Plasmid

#37120

Purpose

DO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cells

Depositor

Bernardo Sabatini

Publication

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

TdTomato-EGFP

Use

AAV and Cre/Lox; Cre-switch

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pT3-EF1a-Bmi1

Plasmid

#31783

Depositor

Xin Chen

Publication

Xu et al Mol Cancer Res. 2009 Dec;7(12):1937-45.

Insert

Bmi1 (BMI1 Human)

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pRRL SFFV d20GFP.T2A.mTagBFP Donor

Plasmid

#31485

Depositor

Andrew Scharenberg

Publication

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d20BFP T2A BFP

Use

Lentiviral

Mutation

GFP has 20 amino acids deleted from the C terminu…

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pET His6 MBP TEV LIC cloning vector (2M-T)

Plasmid

#29708

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, MBP, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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pRsetB-pHRed

Plasmid

#31472

Depositor

Gary Yellen

Publication

Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

Insert

pHRed

Tags

7xHis

Expression

Bacterial

Available since

July 21, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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