We narrowed to 2,906 results for: BFP
-
Plasmid#224484PurposeGateway compatible middle entry clone containing V5 tagged mTagBFP2 (Cytosolic blue fluorescent reporter)DepositorInsertV5-mTagBFP2
UseGateway cloningAvailable SinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3
Plasmid#98974PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 3 and Cas9(D10A)
ExpressionMammalianAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-iCre
Plasmid#113837PurposeMonomeric TagBFP2 and iCre recombinaseDepositorInsertmTagBFP2-T2A-iCre
ExpressionMammalianAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-nCas9n-P2A-TagBFP2-pA
Plasmid#176026PurposeMultisite gateway vector for expression of nCas9n with blue reporter. Parton lab clone ENTDepositorInsertnCas9n-P2A-TagBFP2
UseGateway entry cloneAvailable SinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomTagBFP-SpHis5
Plasmid#44838PurposeYeast tagging vector to create gene fusion with yeast optimized mTagBFP with SpHis5 selectionDepositorInsertyomTagBFP
ExpressionYeastAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-C1-SACM1LdeltaTMD-C389S-Fis1tail
Plasmid#220079PurposeSAC1 with a C389S mutation in it with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue FluorophoreDepositorAvailable SinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-EBFP2-ccdB-Puro
Plasmid#158552PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEBFP2ExpressionMammalianAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn1-TagBFP2/CAAX
Plasmid#197590PurposeLentiviral transfer vector for the neuronal expression of membrane-bound TagBFP2 (good visualization of neuronal processes)DepositorInsertmTagBFP2-CAAX
UseLentiviralTagsCAAXPromoterhSynIAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVH231-Tier1-PmPGK1-mTagBFP2
Plasmid#169520PurposeTier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).DepositorInsertPPGK-driven monomeric Tag blue fluorescent protein 2
ExpressionMammalianPromoterPPGKAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL2-mGold-P2A-EBFP2
Plasmid#157998PurposeCo-expression of mGold and EBFP2 in mammalian cells.DepositorInsertmGold-P2A-EBFP2
ExpressionMammalianMutationmGold is mVenus with L46F;T63S mutationsPromoterCMVAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5135: pCAG-BFP-GuideArray
Plasmid#210606PurposeGuide array for hyperdCas12a-based transcriptional activator with AAAT synSeparatorDepositorInsertpCAG-BFP-GuideArray
UseCRISPRPromoterCAGAvailable SinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-tagBFP-PSM-EGFP
Plasmid#100603PurposeDonor scaffold with EGFP positive selection module, and tagBFP negative selection moduleDepositorTypeEmpty backboneUseSynthetic BiologyTagsEGFP-T2A-Puro and tagBFPAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRepair-MPP8_mAID-P2A-BFP
Plasmid#175036PurposeHomology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9DepositorInsertmAID-P2A-BFP
UseHdr donor templateAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-crScaffold_SV40-BFP
Plasmid#224860PurposecrRNA scaffold for RfxCas13d expressed from hU6 promoter and reporter BFP protein expressed from SV40 promoterDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionMammalianPromoterhU6Available SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)
Plasmid#172066PurposeProtein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intronDepositorInsertmTagBFP2(11x7)
ExpressionInsectAvailable SinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAC-CR7T-gRNA2.1-nlsBFP
Plasmid#170515PurposegRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterCR7TAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha1 P35S:BFP:T35S (GB3416)
Plasmid#160637PurposeTU for the constitutive expression of Blue Fluorescent ProteinDepositorInsertBFP
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4
Plasmid#98975PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 4 and Cas9(D10A)
ExpressionMammalianAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-tagBFP-PSM-dTOMATO
Plasmid#100604PurposeDonor scafold with dTOMATO positive selection module, and tagBFP negative selection moduleDepositorTypeEmpty backboneUseSynthetic BiologyTagsdTOMATO-T2A-Puro and tagBFPAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-dre
Plasmid#124832PurposeMonomeric TagBFP2 and dre recombinaseDepositorInsertmTagBFP2-T2A-dre
ExpressionMammalianPromoterCAGAvailable SinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-EBFP2-ccdB-G418
Plasmid#158556PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEBFP2ExpressionMammalianAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 yeBFP2-T2A-mCherry
Plasmid#219851Purposeequimolar expression of yeBFP2 and mCherry in budding yeastDepositorInsertyeBFP2-T2A-mCherry
ExpressionYeastAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW yomTagBFP2-T2A-mCherry
Plasmid#205493PurposeExpression of yomTagBFP2-T2A-mCherry in yeastDepositorInsertyomTagBFP2-T2A-mCherry
ExpressionYeastAvailable SinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
SOX2-P2A-tagBFP-HDR
Plasmid#163751PurposeHDR knock-in template for tagging the human endogenous SOX2 gene with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.DepositorInsertSOX2 (SOX2 Human)
UseCRISPR and Synthetic BiologyTagsP2A-tagBFPMutationDoes not contain start codon to avoid random inte…Available SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9-SunTag(10X)-2A-BFP
Plasmid#227389PurposeAdhesion module, dCas9-SunTagDepositorInsertdCas9-SunTag
UseLentiviralMutationEnzymatically dead Cas9Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Pou5f1-g2)-PGKpuroBFP-W
Plasmid#105036PurposeLentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-EBFP2-ccdB-Blast
Plasmid#158557PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEBFP2ExpressionMammalianAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mTagBFP2-FRB-Rab7
Plasmid#214273PurposeMammalian expression of mTagBFP2-FRB-Rab7 (late endosome marker)DepositorAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
plenti-arRNA-BFP-backbone
Plasmid#180221PurposeFor the cloning of arRNA-expressing vectors containing BFP fluorescence marker in mammalian cellsDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ST-SBP-tagBFP
Plasmid#65266Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorInsertStr-KDEL and truncated sialyltransferase (ST6GAL1 Human)
TagsStreptavidin Binding Protein (SBP) and tagBFPExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable SinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-W
Plasmid#105034PurposeLentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2
Plasmid#169531PurposeTier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).DepositorInsertPCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2
ExpressionMammalianPromoterPhCMVAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W
Plasmid#67983PurposeCas9 activity reporter (control) with GFP and BFPDepositorInsertU6gRNA cassette, PGKGFP2ABFP cassette, WPRE
UseCRISPR and LentiviralMutationDeleted BbsI site within WPRE, modified BFPAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(GFP)-PGKpuro2ABFP-W
Plasmid#200475PurposeLentiviral vector expressing gRNA targeting GFPDepositorInsertGFP
UseLentiviralAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA EBFP2
Plasmid#167905PurposeLentiviral vector for expressing U6 sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W
Plasmid#105039PurposeLentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(GATA3(13))-PGKpuro2ABFP-W
Plasmid#208548PurposeLentiviral vector expressing gRNA targeting human GATA3DepositorInsertGATA3(13) (GATA3 Human)
UseLentiviralAvailable SinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(GATA3(12))-PGKpuro2ABFP-W
Plasmid#208547PurposeLentiviral vector expressing gRNA targeting human GATA3DepositorInsertGATA3(12) (GATA3 Human)
UseLentiviralAvailable SinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1
Plasmid#98972PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 1 and Cas9(D10A)
ExpressionMammalianAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2
Plasmid#98973PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 2 and Cas9(D10A)
ExpressionMammalianAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Stop-gcry-TagBFP
Plasmid#117771PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsert3x3 Stop sequence
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRE-HRI-V5-IRES-BFP
Plasmid#202435PurposeLentiviral vector expression of HRI and TagBFPDepositorAvailable SinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUltra-hot-H2B-mTagBFP2
Plasmid#161792Purpose3rd generation lentiviral multicistronic vector for mammalian expression of mCherry, H2B-mTagBFP2, and one gene of interestDepositorInsertH2B-mTagBFP2
UseLentiviral; MulticistronicTagsmCherry and mTagBFP2ExpressionMammalianMutationI174A in the mTagBFP2 protein according to PLoS O…PromoterUbCAvailable SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FKBP-mTagBFP2-SOScat
Plasmid#178866PurposeMammalian expression of SOS1(Rem-Cdc25) fused to FKBP-mTagBFP2DepositorAvailable SinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS1-rab-3P::his24::tagBFP2
Plasmid#124344PurposeNuclear Localized Pan-Neuronal tagBFP2DepositorInsertrab3, tagBFP2 (rab-3 Nematode)
ExpressionWormAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
U6::sgBFP-U6::sgKrasG12D-pX333-red
Plasmid#149448PurposepX333 backbone expressing 2 sgRNA targeting BFP and KRAS G12D to edit mouse KRAS G12D allele.DepositorInsertsgRNA BFP and mouse KRAS G12D
UseCRISPRExpressionMammalianAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS1-rab-3P::his-24::tagBFP
Plasmid#124343PurposeNuclear Localized Pan-Neuronal tagBFPDepositorInsertrab-3, tagBFP (rab-3 Nematode)
ExpressionWormAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
StrKDEL-IRES-mannosidase II-mTagBFP2
Plasmid#165460PurposeExpressed mannII-BFP simultaneously with a RUSH Hook Str-KDEL.DepositorInsertPartial ManII-mTag2BFP fusion (Man2a1 Mouse)
TagsStr-KDEL and mTag2BFPExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W
Plasmid#105040PurposeLentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-cmlc2-TagBFP
Plasmid#117786PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertmRFP
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only