164,247 results

1. LZF43: Cre-on CAG-CheRiff-CFP (DIO)

   Plasmid

   #104114

   Purpose

   synOptopatch construct

   Depositor

   Adam Cohen

   Article

   Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

   Insert

   CheRiff-CFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 phiC31

   Plasmid

   #68310

   Purpose

   In vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection).

   Depositor

   Konrad Basler

   Article

   Bischof et al Proc Natl Acad Sci U S A. 2007 Feb 27;10

   Insert

   phiC31
   

   Use

   Rna transcription

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. anxA1-GFP

   Plasmid

   #107194

   Purpose

   Expresses porcine annexin A1-GFP fusion protein for live cell imaging

   Depositor

   Ursula Rescher, Volker Gerke

   Article

   Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

   Insert

   annexin A1
   

   Use

   Tags

   Green Fluorescent Protein

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry

   Plasmid

   #203652

   Purpose

   Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.

   Depositor

   Liam Holt

   Article

   Shu T et et al bioRxiv 2022

   Insert

   PfV
   

   Use

   Lentiviral

   Tags

   Sapphire-IRES-H2B-mCherry

   Expression

   Mutation

   Promoter

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS

   Plasmid

   #71396

   Purpose

   2Gi2R assay

   Depositor

   Fred Gage

   Article

   Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

   Inserts

   2xGFP:NES
   2xRFP:NLS
   

   Use

   Lentiviral

   Tags

   IRES

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha and IRES

   Available Since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDIRECT_23C

   Plasmid

   #91140

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. murine TCR OTII-2A.pMIG II

   Plasmid

   #52112

   Purpose

   retroviral expression of both TCRalpha and TCRbeta using a single vector

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Inserts

   T cell receptor alpha chain (Tcra Mouse)
   T cell receptor beta chain (Tcrb Mouse)
   

   Use

   Retroviral

   Tags

   P2A (from porcine teschovirus-1)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA Raptor

   Plasmid

   #8513

   Depositor

   David Sabatini

   Article

   Kim et al Cell 2002 Jul 26;110(2):163-75.

   Insert

   Raptor (RPTOR Human)
   

   Use

   Tags

   ha

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. a1Ha-pcDNA3

   Plasmid

   #45809

   Depositor

   Edward Perez-Reyes

   Article

   Cribbs et al Circ Res. 1998 Jul 13;83(1):103-9.

   Insert

   a1Ha (CACNA1H Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-link-ymScarletI-URA3

    Plasmid

    #168055

    Purpose

    Replaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymScarletI
    

    Use

    Tags

    ymScarletI

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. AbVec2.0-IGHG4

    Plasmid

    #99578

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG4 isotype

    Depositor

    Hedda Wardemann

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG416GPD-ccdB

    Plasmid

    #14148

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO_TOMM20-SNAP-tag2-T2A-EGFP

    Plasmid

    #226516

    Purpose

    Mamalian cell expression of SNAP-tag2 on mitochondrial surface and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    TOMM20-SNAP-tag2-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-Lyso-TORCAR

    Plasmid

    #64929

    Purpose

    Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    LAMP1-TORCAR (LAMP1 Human, Synthetic)
    

    Use

    Tags

    Cerulean, Full-length LAMP1, and YPet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-hNox4

    Plasmid

    #69352

    Purpose

    expresses human Nox4 in mammalian cells

    Depositor

    Karl-Heinz Krause

    Article

    Serrander et al Biochem J. 2007 Aug 15;406(1):105-14.

    Insert

    hNOX4 (NOX4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. QPM-sgR (pTaU3)

    Plasmid

    #140448

    Purpose

    For sgRNA expression in monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

    Insert

    TaU3-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    TaU3

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTRC-APEX2

    Plasmid

    #72558

    Purpose

    Expression of cytosolic APEX2 in bacteria

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    APEX2
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    A134P relative to APEX

    Promoter

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX-PVX

    Plasmid

    #213040

    Purpose

    PVX vector to express heterologous sequences in plants

    Depositor

    Jose-Antonio Daros

    Article

    Uranga et al Hortic Res. 2023 Dec 19;11(1):uhad279. d

    Insert

    Potato virus X
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S

    Available Since

    May 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJC5-LAMP1-RFP-3xHA

    Plasmid

    #102932

    Purpose

    Express a lysosomal Tag

    Depositor

    David Sabatini

    Article

    Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

    Insert

    LAMP1
    

    Use

    Lentiviral

    Tags

    RFP-HA

    Expression

    Mutation

    Promoter

    ubc

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC57-sgRNA expression vector

    Plasmid

    #51132

    Purpose

    For in vitro transcription of sgRNA from the T7 promoter.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV hSyn FLEx-loxp Kir2.1-2A-GFP

    Plasmid

    #161574

    Purpose

    Expression of Kir2.1-2A-GFP in a Cre-dependent fashion

    Depositor

    Kevin Beier, Robert Malenka

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    Kir2.1-2A-GFP (Kcnj2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti CMV GFP DEST (736-1)

    Plasmid

    #19732

    Purpose

    3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expression

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi; Gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX330S-3

    Plasmid

    #58779

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. lenti SYN-FLAG-dCas9-VPR

    Plasmid

    #114196

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SYN

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-FLuc

    Plasmid

    #170575

    Purpose

    Express firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay.

    Depositor

    David Nemazee

    Article

    Rogers et al Science. 2020 Aug 21;369(6506):956-963.

    Insert

    Luc
    

    Use

    Luciferase and Retroviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPB-cT3G-cERP2-NR5A1

    Plasmid

    #192915

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of NR5A1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    NR5A1 (NR5A1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3 Flag TSC2

    Plasmid

    #14129

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 (TSC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. FLAG-HA-pcDNA3.1-

    Plasmid

    #52535

    Purpose

    enables eukaryotic expression of N-terminally FLAG-HA-tagged fusion proteins

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUCmini-iCAP-AAV.MaCPNS1

    Plasmid

    #185136

    Purpose

    non-standard AAV2 rep-AAV-MaCPNS1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

    Insert

    Synthetic construct isolate AAV-MaCPNS1 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFP-NUP98

    Plasmid

    #229593

    Purpose

    Expresses EGFP-NUP98 fusion in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HA-APP-mGFP

    Plasmid

    #196696

    Purpose

    Expresses APP695 with an HA tag an N-terminal for single molecule tracking and other measusers in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    HA and mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV2-SEP-GluA1 (M1)

    Plasmid

    #64942

    Purpose

    mammalian expression of rat GluA1

    Depositor

    Jeremy Henley

    Article

    Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

    Insert

    GluA1 (Gria1 Rat)
    

    Use

    Tags

    SEP (superecliptic pHluorin)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSG5 PPAR alpha

    Plasmid

    #22751

    Depositor

    Bruce Spiegelman

    Insert

    PPARalpha (Ppara Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28a-MH6-Bsu

    Plasmid

    #163911

    Purpose

    Expresses Bsu large fragment for affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    Bsu polymerase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pUCmini-iCAP-PHP.B

    Plasmid

    #103002

    Purpose

    non-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCDNA3.1-YY1

    Plasmid

    #169018

    Purpose

    Expression of YY1 driven by CMV promoter

    Depositor

    Claes Wadelius

    Article

    Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

    Insert

    human YY1 (YY1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. EF.STAT3C.Ubc.GFP

    Plasmid

    #24983

    Purpose

    3rd generation lentiviral expression of constitutively active Stat3

    Depositor

    Linzhao Cheng

    Article

    Hillion et al Cancer Research 68, 10121, December 2008

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    A662C, N664C (constitutively active)

    Promoter

    Available Since

    May 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pKM468-EGFP

    Plasmid

    #108434

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with EGFP.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    attB-eGFP-4xGly-TEV-Flag-6xHis
    

    Use

    Orbit integrating plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mTFP1-N1

    Plasmid

    #54521

    Purpose

    Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

    Depositor

    Robert Campbell, Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Type

    Empty backbone

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. drebrin-YFP

    Plasmid

    #40359

    Depositor

    Phillip Gordon-Weeks

    Article

    Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

    Insert

    drebrin E2 (DBN1 Human)
    

    Use

    Chick expression

    Tags

    eYFP

    Expression

    Mutation

    Promoter

    chick B-actin promoter with CMV enhancer

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge