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Plasmid#194697PurposeHuC driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression in zebrafishDepositorInsertCaprola_on-mEGFP
UseZebrafish expressionTagsmEGFPExpressionMutationPromoterelavl3(HuC)Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2_elavl3(HuC)_NES-Caprola_off-mEGFP
Plasmid#194698PurposeHuC driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression in zebrafishDepositorInsertCaprola_off-mEGFP
UseZebrafish expressionTagsmEGFPExpressionMutationPromoterelavl3(HuC)Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-20XUAS-IVS-NES_Caprola5-mEGFP
Plasmid#205704PurposeGal4 driven expression of EGFP-Caprola5 for targeted expression in Drosophila fliesDepositorInsertCaprola5-EGFP
UseTagsExpressionInsectMutationPromoter20 Gal4Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
plx208-CMV-SspB-EGFP-VP16
Plasmid#213532PurposeExpresses one of the split transcription factor component in mammalian cellsDepositorInsertSspB-EGFP-VP16
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K48R/K63R)-yeGFP
Plasmid#212769PurposeC-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
px552-U6-gRNA1-U6-gRNA2-CMV-eGFP
Plasmid#211760PurposePaired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.DepositorInsertgRNA1 and gRNA2 targeting VEGF-A (VEGFA Mouse, M. mulatta (rhesus macaque), Human)
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCaUra3-CUbo(K48R/K63R)-yeGFP
Plasmid#212774PurposeC-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with CaUra3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K48R)-yeGFP-His6
Plasmid#212783PurposeC-terminal PCR-based tagging with CUbo(K48R)-yeGFP-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K63R)-yeGFP-His6
Plasmid#212784PurposeC-terminal PCR-based tagging with CUbo(K63R)-yeGFP-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFR*-CUbo-GFP
Plasmid#212815PurposeConstitutive or doxycycline-inducible expression of EGFR*-CUbo-GFP in mammalian cellsDepositorInsertEGFR* (EGFR Budding Yeast, Human)
UseTags8xHis and CUbo-GFPExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFR*-CUbo-His6
Plasmid#212819PurposeConstitutive or doxycycline-inducible expression of EGFR*-CUbo-His6 in mammalian cellsDepositorInsertEGFR* (EGFR Budding Yeast, Human)
UseTagsCUbo-6xHisExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFR*-CUbo(K63R)
Plasmid#212822PurposeConstitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R) in mammalian cellsDepositorInsertEGFR* (EGFR Budding Yeast, Human)
UseTagsCUbo(K63R)ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-53~77-mEGFP(A206K)-TwStrep
Plasmid#208632Purposefor E. coli expression truncated human RPL3 (ENST00000216146), aa53~77, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 53~77 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 53~77, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-1~77-mEGFP(A206K)-TwStrep
Plasmid#208624Purposefor E. coli expression truncated human RPL3 (ENST00000216146), aa1~77, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 1~77 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 1~77, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN0617 (pDEST-mEGFP C-term, NatR)
Plasmid#210484PurposepDEST vector to tag gene of interest with mEGFP on the C-terminus to perform localization assays in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gatewayā¦TagsmEGFPExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Talin head-SpyCatcher003(K31TAG)
Plasmid#210023PurposeExpression of EGFP-Talin head(1-433)-SpyCatcher003(K31TAG) in mammalian cellsDepositorInsertEGFP-Talin head (1-433)-SpyCatcher003(K31TAG)
UseTagsExpressionMammalianMutationSpyCatcher003 with K31TAG (Amber stop codon) mutaā¦PromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSSA-DsRed/eGFP (pXK-442)
Plasmid#208827PurposeThe SSA-based surrogate reporter for validating the activity of different gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CMV-DsRed-CMV-eGFP*(SSA).DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pminiTol2-4kb wnt3 promoter-Wnt3EGFP
Plasmid#203793PurposeTol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of Wnt3-EGFP for zebrafish transgenesisDepositorInsertWnt3 (wnt3 Zebrafish)
UseZebrafish expressionTagsmEGFPExpressionMutationPromoter4kb Wnt3 promoterAvailable sinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.7_Gag-dZF2-MCP-P2A-eGFP
Plasmid#205523PurposeExpress HIV Gag-delta-ZF2-MCP-P2A-eGFPDepositorInsertGag delta ZF2-MCP
UseTagsExpressionMammalianMutationZF2 deletedPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.14_GagZip-dp1dp6-MCP-P2A-eGFP
Plasmid#205529PurposeExpress HIV GagZip-dp1dp6-MCP-P2A-eGFPDepositorInsertGagZip-dp1dp6-MCP
UseTagsExpressionMammalianMutationNucleocapsid replaced with leucine zipper, p1 andā¦PromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.17_GagZip-MCP-dpol-P2A-eGFP
Plasmid#205532PurposeExpress HIV GagZip-MCP-dpol-P2A-eGFPDepositorInsertGag-MCP-dpol
UseTagsExpressionMammalianMutationNucleocapsid replaced with leucine zipper, pol frā¦PromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
F16-pAc5.1 EGFP Vasa closed
Plasmid#206487PurposeVasa (closed conformation) expression for Schneider cell imagingDepositorInsertvasa E400Q
UseTagsEGFPExpressionInsectMutationE400QPromoterAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-miRFP704nano(S86C)-P2A-EGFP
Plasmid#197372PurposeThe lentiviral vector for miRFP704nano(S86C) and EGFPDepositorInsertmiRFP704nano(S86C)
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationS86C mutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-miRFP718nano(S86C)-P2A-EGFP
Plasmid#197373PurposeThe lentiviral vector for miRFP718nano(S86C) and EGFPDepositorInsertmiRFP718nano(S86C)
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationS86C mutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-iRFP713(V256C)-P2A-EGFP
Plasmid#198061PurposeThe lentiviral vector for iRFP713(V256C) and EGFPDepositorInsertiRFP713(V256C)
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationV256C mutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-NIR-GECO2-P2A-EGFP
Plasmid#198063PurposeThe lentiviral vector for NIR-GECO2 and EGFPDepositorInsertNIR-GECO2
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-NIR-GECO1-P2A-EGFP
Plasmid#198065PurposeThe lentiviral vector for NIR-GECO1 and EGFPDepositorInsertNIR-GECO1
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT1-d2EGFP-mCherry
Plasmid#201451PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT1_d2EGFP-mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT2-d2EGFP-mCherry
Plasmid#201452PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT2_d2EGFP-mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT4-d2EGFP-mCherry
Plasmid#201453PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT4_d2EGFP-mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT5-d2EGFP-mCherry
Plasmid#201454PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT5_d2EGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT mCh-V5-NES-EGFP-mCBP80
Plasmid#192313PurposeGateway entry vector for an inducible mCh-V5-NES-EGFP-mCBP80DepositorInsertmCh-V5-NES-EGFP-mCBP80
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT mCh-V5-NES-EGFP-IBB
Plasmid#192314PurposeGateway entry vector for an inducible mCh-V5-NES-EGFP-IBBDepositorInsertmCh-V5-NES-EGFP-IBB
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K
Plasmid#198037PurposeUsed for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)DepositorInsertTBK1 (TBK1 Human)
UseTagsGST-TEV-EGFPExpressionInsectMutationE696KPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sgRNA(OXTR.2)-CMV-eGFP
Plasmid#194016PurposeExpresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFPDepositorInsertssgRNA(Oxtr.2)
eGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromotercmb and u6Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGIGYF-del331-370_AH
Plasmid#148921PurposeInsect Expression of DmGIGYF-del331-370DepositorInsertDmGIGYF-del331-370 (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence giveā¦PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-EGFP-DmRoquin_702-819_AE
Plasmid#148604PurposeInsect Expression of DmRoquin_702-819DepositorInsertDmRoquin_702-819 (roq Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmRoquin-del725-755_AE
Plasmid#148590PurposeInsect Expression of DmRoquin-del725-755DepositorInsertDmRoquin-del725-755 (roq Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmRoquin-del790-812_AE
Plasmid#148592PurposeInsect Expression of DmRoquin-del790-812DepositorInsertDmRoquin-del790-812 (roq Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-EGFP-DmRoquin_501-702_AD
Plasmid#148529PurposeInsect Expression of DmRoquin_501-702DepositorInsertDmRoquin_501-702 (roq Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only