We narrowed to 31,549 results for: Eng;

1. pAAV-hSyn-Cepheid1s-ST-WPRE

   Plasmid

   #214968

   Purpose

   Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for better photostability

   Depositor

   Peng Zou

   Article

   Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

   Insert

   Cepheid1s-ST
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human Synapsin

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSyn-Cepheid1b-ST-WPRE

   Plasmid

   #214967

   Purpose

   Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for brighter fluorescence

   Depositor

   Peng Zou

   Article

   Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

   Insert

   Cepheid1b-ST
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human Synapsin

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p7SL-neo-TET

   Plasmid

   #51285

   Purpose

   expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette

   Depositor

   Astrid Roy-Engel

   Article

   Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

   Insert

   7SL RNA (RN7SL2 Human)
   

   Use

   Tags

   neoTET cassette with a tetrahymena self-splicing …

   Expression

   Mammalian

   Mutation

   Promoter

   7SL upstream pollII enhancer

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCrysβ:ECFP-LexOP:mCherry-KrasG12V

   Plasmid

   #89888

   Purpose

   Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   mCherry-KRasG12V
   

   Use

   Unspecified

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA4/TO-HRASG12V

   Plasmid

   #216509

   Purpose

   Expresses HRAS with a G12V point mutation in human cells under a tetracycline/doxycycline-inducible promoter

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   HRAS (HRAS Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. scaffold-linker-U6

   Plasmid

   #89639

   Purpose

   construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

   Depositor

   Wensheng Wei

   Article

   Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

   Plasmid

   #110201

   Purpose

   Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   LexPR transactivator - 2A - Cerulean
   

   Use

   Tags

   Cerulean

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-hSyn-GRAB_NE1h

   Plasmid

   #123309

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neurons

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

   Has Service

   AAV Retrograde and AAV9

   Insert

   GPCR activation based NE sensor GRAB_NE1h
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. sg_Brd9_i5-ipUSEPR-TR657

   Plasmid

   #228922

   Purpose

   Knockdown of Brd9 in mammalian cells

   Depositor

   Sika Zheng

   Article

   Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

   Insert

   sg_Brd9_i5 (Brd9 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. sg_Brd9_i2-ipUSEPR-TR657

    Plasmid

    #228921

    Purpose

    Knockdown of Brd9 in mammalian cells

    Depositor

    Sika Zheng

    Article

    Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

    Insert

    sg_Brd9_i2 (Brd9 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-sgRNA-lib 2.0

    Plasmid

    #89638

    Purpose

    sgRNA expression in mammalian cells after Gateway cloning

    Depositor

    Wensheng Wei

    Article

    Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pENTR221 UBR5

    Plasmid

    #81062

    Purpose

    Gateway entry vector encoding full length UBR5

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) (UBR5 Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    K503R

    Promoter

    N/A

    Available since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-Myc CDC4 WT*

    Plasmid

    #16652

    Depositor

    Bert Vogelstein

    Article

    Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

    Insert

    CDC4 (FBXW7 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    *Note that this insert is only WT relative to the…

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SpRYc

    Plasmid

    #175575

    Purpose

    Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells

    Depositor

    Joseph Jacobson

    Article

    Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:

    Insert

    SpRYc
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    PID of SpRY grafted onto the N-terminal domain of…

    Promoter

    CMV

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTT3 Unc5B-FLAG

    Plasmid

    #72195

    Purpose

    Expresses full-length Unc5b with a C-terminal FLAG tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5b (Unc5b Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFBD-Akt13C(S473D)

    Plasmid

    #86577

    Purpose

    Expresses cleavable human full-length Akt1(S473D) phosphomimetic

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    10xHis

    Expression

    Insect

    Mutation

    3C cleavage site inserted at pos. 130; S473D phos…

    Promoter

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX459-TP53-exon4

    Plasmid

    #217455

    Purpose

    For TP53 knockout targeting exon 4 of TP53

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    CRISPR Cas9 guide for TP53 (TP53 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTT3 Unc5A-FLAG

    Plasmid

    #72194

    Purpose

    Expresses full-length Unc5a with a C-terminal FLAG tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5a (Unc5a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-hSyn-GRAB_NEmut

    Plasmid

    #123310

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neurons

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Has Service

    AAV Retrograde and AAV9

    Insert

    GPCR activation based NE control sensor GRAB_NEmut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PX459-TP53-exon5

    Plasmid

    #217456

    Purpose

    For TP53 knockout targeting exon 5 of TP53

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    CRISPR Cas9 guide for TP53 (TP53 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Src-Y530F

    Plasmid

    #124659

    Purpose

    Human c-Src with Y530F mutation

    Depositor

    Cheng-han Yu

    Article

    Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

    Insert

    cSrc (SRC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y530F

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mEosEM-E_Lamin A

    Plasmid

    #226338

    Purpose

    Photoswitchable fluorescent protein for correlative light-electron microscopy; nuclear envelope-targeted mEosEM-E for mammalian expression

    Depositor

    Pingyong Xu

    Article

    Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

    Insert

    Lamin A (LMNA Human)
    

    Use

    Tags

    mEosEM-E

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUC19_Ntag_BbsI-Mammalian

    Plasmid

    #186674

    Purpose

    N-terminal tag cassette with BbsI restriction sites for CRISPR donor plasmid.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    Mammalian N-terminal Tag
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA3-HA-Akt1-K179M

    Plasmid

    #73409

    Purpose

    Expresses kinase-inactive mutant of Akt with HA tag

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    changed K179M to make Kinase inactive mutant

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTT3 Unc5D-FLAG

    Plasmid

    #72197

    Purpose

    Expresses full-length Unc5d with a C-terminal FLAG tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5d (Unc5d Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Tol2-lyzC-mcherry-2a-CDK2

    Plasmid

    #121131

    Purpose

    neutrophil specific expression

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    dre-WT CDK2 (cdk2 Zebrafish)
    

    Use

    Tags

    mcherry-2a

    Expression

    Bacterial

    Mutation

    Promoter

    neutrophil specific lyzC

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pU6-neo-TET

    Plasmid

    #51286

    Purpose

    expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    U6 (RNU6-1 Human)
    

    Use

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Mutation

    Promoter

    U6 upstream enhancer sequence

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SOX17-3'gRNA

    Plasmid

    #210465

    Purpose

    gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    SOX17 (SOX17 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Ai32

    Plasmid

    #34880

    Purpose

    Cre-dependent expression of ChR2(H134R)-EYFP, for optogenetic excitation

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

    Insert

    Floxed ChR2(H134R)-EYFP
    

    Use

    Mouse Targeting

    Tags

    EYFP

    Expression

    Mutation

    H134R in ChR2

    Promoter

    CAG

    Available since

    May 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGL410-BRN2p

    Plasmid

    #110733

    Purpose

    Luciferase reporter for the human BRN2 promoter

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    BRN2 promoter luciferase (POU3F2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCDNA3-HA-Akt1-aa1-149

    Plasmid

    #73410

    Purpose

    Expresses HA tagged aa1-149 of AKT1

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Expresses aa 1-149 only of AKT1

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

32. pcDNA3.1(+)- e18 LOF

    Plasmid

    #131729

    Purpose

    Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

    Depositor

    Alberto Ciccia

    Article

    Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

    Insert

    e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCrysβ:ECFP-LexOP:mCherry-HrasG12V

    Plasmid

    #89887

    Purpose

    Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    mCherry-HRasG12V
    

    Use

    Unspecified

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCDNA3-HA-Akt1-aa120-433

    Plasmid

    #73411

    Purpose

    Expresses HA tagged aa120-433 AKT1

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Expresses aa 120-433 of AKT1

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

35. pCrysβ:ECFP-LexOP:mCherry-NRasQ61K

    Plasmid

    #89886

    Purpose

    Human NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    mCherry-NRasQ61K
    

    Use

    Unspecified

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TOP2A sgRNA1

    Plasmid

    #138190

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TOP2A

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TOP2A sgRNA2

    Plasmid

    #138191

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TOP2A

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAC149-pCR8-dCas9VP160

    Plasmid

    #48221

    Purpose

    dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP160

    Expression

    Mutation

    D10A;H840A nuclease-deficient

    Promoter

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLNCX2-STIM1

    Plasmid

    #89817

    Purpose

    Express HUMAN STIM1

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    NO TAG

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDNA3-HA-Akt1-aa1-408

    Plasmid

    #73412

    Purpose

    Expresses HA tagged aa1-408 of AKT1

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Expresses aa 1-408 of AKT1

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only