We narrowed to 29,737 results for: REP

1. pEF1α:flex-mcherry

   Plasmid

   #222618

   Purpose

   Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

   Depositor

   Franck Polleux

   Article

   Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

   Insert

   Cre-dependent mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuet-1_hGH

   Plasmid

   #182410

   Purpose

   Bacterial expression of human growth hormone

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   hGH (GH1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS_4xMma PylT_FLAG-AcKRS

   Plasmid

   #140021

   Purpose

   Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   AcKRS-3 (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   L301M, L305I, Y306F, L309A, C348F

   Promoter

   EF1

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST40-2XFL-Src-K298M-Y419F-Y530F

   Plasmid

   #140321

   Purpose

   Expresses Flag-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Use

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   K298M-Y419F-Y530F

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

   Plasmid

   #140018

   Purpose

   Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   EcoTyrRS
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y37L, D182S, F183M, L186A

   Promoter

   EF1

   Available Since

   May 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

   Plasmid

   #174899

   Purpose

   Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGM22082_cRed

   Plasmid

   #117225

   Purpose

   pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

   Insert

   Canthaxanthin Operon
   

   Use

   Tags

   34 amino acid N-terminal linker, Hexahistidine Ta…

   Expression

   Bacterial

   Mutation

   removed Type IIS restriction sites within the bac…

   Promoter

   T7 RNA Polymerase

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSFDuet-1_mNeongreen-Etanercept

   Plasmid

   #182420

   Purpose

   Bacterial expression of mNeongreen-Etanercept fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   mNeongreen-Etanercept (TNFRSF1B Human)
   

   Use

   Tags

   mNeongreen

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS_4xG1 PylT FLAG-G1 PylRS Y125A

   Plasmid

   #154769

   Purpose

   plasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y125A

   Promoter

   EF1

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Bxb1_AJMA

    Plasmid

    #183760

    Purpose

    Bxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Inserts

    ASAP2f
    jRCaMP1b
    miRFP703
    ASAP2f
    

    Use

    Tags

    Lck and NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFL-EGFP-3xFlag-DEST

    Plasmid

    #134903

    Purpose

    Baculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tag

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Type

    Empty backbone

    Use

    Tags

    3x-Flag

    Expression

    Insect

    Mutation

    Promoter

    polyhedron

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB-CAG-FLEX-F14F15-BFP-(mCherry)'

    Plasmid

    #201831

    Purpose

    piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

    Depositor

    Ian Wickersham

    Article

    Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Inserts

    CAG
    BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA 3.3 hFZD4

    Plasmid

    #115908

    Purpose

    Expresses human FZD4 in mammalian cells.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    FZD4 (FZD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-eGFP-P2A-LgBiT-NLS

    Plasmid

    #187834

    Purpose

    Expression of eGFP-P2A-LgBiT-NLS from human synapsin promoter via adeno-associated virus for neuronal expression.

    Depositor

    William McEwan

    Article

    Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

    Insert

    eGFP-P2A-LgBiT-NLS
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-W

    Plasmid

    #105043

    Purpose

    Lentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Stk11 (Stk11 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-KS-attB2-SA(2)-T2A-dVP16AD-Hsp70

    Plasmid

    #62911

    Purpose

    Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-dVP16AD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-FRT/TO-mNeonGreen-BRD4

    Plasmid

    #204692

    Purpose

    This plasmid allows for inducible expression of short BRD4 isoform tagged with mNeonGreen, in mammalian cells.

    Depositor

    Michael Rosen

    Article

    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR221-YAP1

    Plasmid

    #79503

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    no Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS-KS-attB2-SA(0)-T2A-LexA::QFAD-Hsp70

    Plasmid

    #62947

    Purpose

    Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-LexA::QFAD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-GFP-WT1 +KTS N terminal HA

    Plasmid

    #217639

    Purpose

    Entry vector with WT1 +KTS mutant with N terminal HA for gateway cloning

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    WT1 +KTS isoform (WT1 Human)
    

    Use

    Gateway cloning

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pENTR-GFP-WT1 -KTS N terminal HA

    Plasmid

    #217641

    Purpose

    Entry vector with WT1 -KTS mutant with N terminal HA for gateway cloning

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    WT1 -KTS isoform (WT1 Human)
    

    Use

    Gateway cloning

    Tags

    HA

    Expression

    Mutation

    p.408_410delKTS

    Promoter

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3-TORCAR-NLS

    Plasmid

    #64931

    Purpose

    Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR-NLS (EIF4EBP1 Synthetic, Human)
    

    Use

    Tags

    Cerulean, Nuclear localization signal (NLS), and …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBS-KS-attB2-SA(0)-T2A-Gal4-Hsp70

    Plasmid

    #62896

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE

    Plasmid

    #168107

    Purpose

    PiggyBac transposon vector constitutively driving PuroR-eGFP and conditionally expressing S.aureus Cas9 and mCherry

    Depositor

    Erich Jarvis

    Article

    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

    Insert

    Cas9-P2A-mCherry
    

    Use

    CRISPR and Cre/Lox

    Tags

    HA

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET17b-yebST

    Plasmid

    #175804

    Purpose

    Complementation of yebST knockout in E. coli K-12

    Depositor

    Ian Henderson, Damian Ekiert, Gira Bhabha

    Article

    Isom et al Sci Rep. 2017 Aug 17;7(1):8608. doi: 10.

    Insert

    yebST
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    silent mutations to introduce restriction sites

    Promoter

    T7

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pRetroX-PTuner DD-linker-M.EcoGII-v5-Lamin B1

    Plasmid

    #122083

    Purpose

    Express M.EcoGII fused to LaminB1 with detabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII-V5-LaminB1
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of LaminB1

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. pJP-CoV2leader-nLuc-TSS

    Plasmid

    #175430

    Purpose

    Expresses nLuc with 5' leader of CoV2 in mammalian cells.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nLUC plus 5' CoV2 leader
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBS-KS-attB2-SA(0)-T2A-p65AD-Hsp70

    Plasmid

    #62912

    Purpose

    Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-p65AD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPB12A

    Plasmid

    #210035

    Purpose

    Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas12a
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. pAAVS1-Puro-TNNT2-Neo

    Plasmid

    #214013

    Purpose

    Expression of cardiac troponin T

    Depositor

    Paul Burridge

    Article

    Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

    Insert

    TNNT2 (TNNT2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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