163,606 results

1. pEGFP-IQGAP1

   Plasmid

   #30112

   Depositor

   David Sacks

   Article

   Ren et al J Biol Chem. 2005 Oct 14. 280(41):34548-

   Insert

   IQGAP1 (IQGAP1 Human)
   

   Use

   Tags

   tandem EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. XRE-pNL1.3[secNLuc]

   Plasmid

   #182295

   Purpose

   Secreted Luciferase reporter for AhR activity

   Depositor

   Severine Degrelle, Thierry Fournier

   Article

   Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

   Insert

   Xenobiotic Response Element (CYP1A1 Human)
   

   Use

   Tags

   secNluc

   Expression

   Mammalian

   Mutation

   Promoter

   XRE

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-Bmpr2

   Plasmid

   #163403

   Purpose

   Expresses BMPR-2 in mammalican cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

   Plasmid

   #62723

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV1 and AAV5

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   human synapsin promoter

   Available Since

   April 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEX_305

   Plasmid

   #41390

   Purpose

   Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag

   Depositor

   David Root

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Nrxn3b-Nav1.6-MKII-f-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

   Plasmid

   #163031

   Purpose

   FLiCRE TF with fast kinetics for neuronal expression

   Depositor

   Alice Ting

   Article

   Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

   Insert

   Nrxn3b-MKII-f-hLOV1-TEVcs-tTA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RfxCas13d-backbone

   Plasmid

   #165078

   Purpose

   Expression of RfxCas13d sgRNAs from a U6 promoter

   Depositor

   Ling-Ling Chen

   Article

   Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

   Insert

   Scaffold gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE

   Plasmid

   #60906

   Purpose

   The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imaging

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO.1 mito-mEmerald

   Plasmid

   #174542

   Purpose

   labels mitochondria with mEmerald

   Depositor

   Minna Roh-Johnson

   Article

   Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

   Insert

   MTS-mEmerald
   

   Use

   Lentiviral

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. rAAV9-CAP-in-cis-Lox-AAV

    Plasmid

    #205990

    Purpose

    rAAV9 cap acceptor vector for CREATE libraries

    Depositor

    Viviana Gradinaru

    Article

    Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

    Insert

    rAAV9 cap acceptor vector for CREATE libraries
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Deletion between XbaI and AgeI sites

    Promoter

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEF1-alpha-V

    Plasmid

    #27290

    Depositor

    Timothy Springer

    Article

    Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

    Insert

    integrin alpha V (ITGAV Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Isoform 2

    Promoter

    Available Since

    March 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLIX403_deltaUBA_UBAP2L_mCherry

    Plasmid

    #105287

    Purpose

    puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein lacking the UBA domain of UBAP2L

    Depositor

    Eugene Yeo

    Article

    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

    Insert

    UBAP2L (UBAP2L Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deleted amino acids 20-108

    Promoter

    TRE promoter, Tet ON

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Stat1 alpha Flag pRc/CMV

    Plasmid

    #8691

    Depositor

    Jim Darnell

    Article

    Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

    Insert

    STAT1 alpha (STAT1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

    Plasmid

    #52521

    Purpose

    Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virus

    Depositor

    Scott Sternson

    Article

    Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

    Insert

    ChR2-2a-hM4D
    

    Use

    AAV and Cre/Lox

    Tags

    2xHA on ChR2

    Expression

    Mutation

    Promoter

    CBA

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.CAG.(cyto).cpSFGFP.HaloTag

    Plasmid

    #209654

    Purpose

    Non-responsive control

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    cpSFGFP.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP VAMP7 (102-220)

    Plasmid

    #42314

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Coiled Coil domain (102-220) (VAMP7 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Δ1-102

    Promoter

    CMV

    Available Since

    March 13, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pC081: pAAV.CMV.mCherry

    Plasmid

    #205744

    Purpose

    AAV vector for expressing CMV-driven mCherry reporter gene.

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1A eIF4A3

    Plasmid

    #83035

    Purpose

    mammalian expression of eIF4A3

    Depositor

    Gideon Dreyfuss

    Article

    Chan et al RNA. 2004 Feb;10(2):200-9.

    Insert

    eIF4A3 (EIF4A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pMSCV-Cas9-2A-GFP-sgRNA

    Plasmid

    #124889

    Purpose

    Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

    Depositor

    Harvey Lodish

    Article

    Li et al Haematologica. 2016 Jun;101(6):e216-9. d

    Insert

    Cas9
    

    Use

    Retroviral

    Tags

    2A-GFP

    Expression

    Mutation

    Promoter

    MSCV

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti Dest BlastR R1-R2

    Plasmid

    #84574

    Purpose

    Gateway destination vector expressing Blasticidin resistance under a PGK promoter

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Type

    Empty backbone

    Use

    Lentiviral; Gateway

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 35S-GAL4BD-GWY/pAM

    Plasmid

    #201233

    Purpose

    Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    35S-GAL4BD-GWY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDONR223_TRAP1_WT_V5

    Plasmid

    #82994

    Purpose

    Gateway Donor vector containing TRAP1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, Matthew Meyerson

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    TRAP1 (TRAP1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDEST_EWSR1

    Plasmid

    #26377

    Depositor

    Thomas Tuschl

    Article

    Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA3-Clover

    Plasmid

    #40259

    Purpose

    GFP with robust performance in protein fusions and useful as FRET donor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Insert

    Clover GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available Since

    Sept. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA5/FRT/TO HSPA8

    Plasmid

    #19460

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPA8 (HSPA8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDule-tfmF A65V S158A

    Plasmid

    #85484

    Purpose

    Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

    Insert

    tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32V F108W Q109M I159M with 65Val and 158Ala

    Promoter

    lpp (constitutive)

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. gRNA NIA TLS2

    Plasmid

    #196981

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs fused to TLS2 tags
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pU6-26, pU6-29

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TetOn-mCherry-eGFP-RAMP4

    Plasmid

    #109014

    Purpose

    Tet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    RAMP4 (SERP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pOT_2 - lenti-EFS-tNGFR-2A-puro

    Plasmid

    #181971

    Purpose

    Expresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    tNGFR (NGFR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    truncation: aa 11-276

    Promoter

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJM483

    Plasmid

    #52987

    Purpose

    A secreted Lactadherin (sGFP::LactC1C2) Phosphatidylserine sensor

    Depositor

    Ding Xue

    Article

    Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

    Insert

    Lactaherin (Mfge8 Mouse)
    

    Use

    Tags

    GFP (w/ introns) and signal sequence

    Expression

    Worm

    Mutation

    deleted native signal sequence, deleted C-termina…

    Promoter

    hsp-16.41

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-U6-gRNA-CBh-mCherry

    Plasmid

    #91947

    Purpose

    Expression of empty gRNA cassette and mCherry

    Depositor

    Jimok Kim

    Article

    Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AAV-U6gRNA1-U6gRNA2-TnT-Cre

    Plasmid

    #87682

    Purpose

    AAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.

    Depositor

    William Pu

    Article

    Guo et al Circ Res. 2017 Mar 29. pii: CIRCRESAHA.1

    Inserts

    gRNA1
    gRNA2
    Cre
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    U6 and cTnT

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pClneoMyc-LATS1

    Plasmid

    #66851

    Purpose

    Express Myc-tagged LATS1 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    LATS1 (LATS1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P44L mutation in LATS1 compared to GenBank refere…

    Promoter

    CMV

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEGFP-N1-MITF-M

    Plasmid

    #38131

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    MITF-M (MITF Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available Since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCAG mito-RCaMP1h

    Plasmid

    #105013

    Purpose

    mitochondrial calcium sensor

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    RCaMP1h
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. EFS-GFP

    Plasmid

    #110834

    Purpose

    positive control for GFP expression

    Depositor

    Michael Lewis

    Article

    Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

    Insert

    elongation factor 1alpha binding sequence (EFS) at GFP promoter region
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. hAAVS1 1R TALEN

    Plasmid

    #35432

    Purpose

    TALEN designed to target the human AAVS1 locus

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    hAAVS1 1R TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. piggyBac-rtTA (4th_Gen)-NGN2-2A-PURO-IRES-SNAP (VK_1018)

    Plasmid

    #209077

    Purpose

    Ngn2 mediated cortical neuron induction, dox induction is included

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Inserts

    NGN2 (Neurog2 Mouse)
    SNAPtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE4G

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. hAAVS1 1L TALEN

    Plasmid

    #35431

    Purpose

    TALEN designed to target the human AAVS1 locus

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    hAAVS1 1L TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLenti PGK Neo DEST (w531-1)

    Plasmid

    #19067

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Neo

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pUC18

    Plasmid

    #50004

    Purpose

    pUC cloning vector

    Depositor

    Joachim Messing

    Article

    Norrander et al Gene. 1983 Dec;26(1):101-6.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pBabe-tetoff-mVenus-PICdc42

    Plasmid

    #91876

    Purpose

    PI(WT): Photo inhibitable (blue light sensitive)

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Insert

    Vav2 (VAV2 Human)
    

    Use

    Tags

    mVenus

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAAV-pCAG eGFP T2A SP HALO HAPLN1

    Plasmid

    #228200

    Purpose

    AAV vector expressing GFP and HaloTag-HAPLN1 under constitutive promoter

    Depositor

    Sungjin Park

    Article

    Sterin et al J Neurosci. 2024 Sep 9:e0666242024. doi:

    Insert

    HAPLN1 (Hapln1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    constitutive CAG promoter

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. FKBP-V5-AP-nes_pLX304

    Plasmid

    #120912

    Purpose

    Lentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosol

    Depositor

    Alice Ting

    Article

    Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

    Insert

    FKBP-V5-AP-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pDest-566

    Plasmid

    #11517

    Depositor

    Dominic Esposito

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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46. CD9-mEGFP

    Plasmid

    #182864

    Purpose

    Localization: Tetraspanin/Membrane

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    human CD9 (CD9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. PX552

    Plasmid

    #60958

    Purpose

    pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

    Depositor

    Feng Zhang

    Article

    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

    Inserts

    U6_(SpaI)_sgRNA
    Syn_EGFP-KASH
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin and U6

    Available Since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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48. pGWB414

    Plasmid

    #74808

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. pMK243 (Tet-OsTIR1-PURO)

    Plasmid

    #72835

    Purpose

    Expresses OsTIR1 under the control of the Tet promoter

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    AAVS1-Tet-OsTIR1-PURO-AAVS1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

    Plasmid

    #185913

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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