We narrowed to 15,045 results for: NOL
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Plasmid#73284PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-CPR
Plasmid#126742Purposeyeast plasmid overexpressing CPRDepositorInsertcpr
UseTags6xHisExpressionYeastMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBR322-bom
Plasmid#155178PurposepBICI_2_strong without bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL705
Plasmid#140375PurposeExpresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoterDepositorInsertT7-5BP-tetO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EK0313 SFFV-mCherry-SG(s70587)SG-Cre (FLP-IN)
Plasmid#191149PurposeExpression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, Cre output derived from a gift from prof. Liqun LuoDepositorInsertmCherry:s70587:Cre
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
IBMc071
Plasmid#161943PurposeContains Type IIS restriction site free elements for expressing protein to be split in IBM. ECF20 expression plasmidDepositorInsertpSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOCC93
Plasmid#118911Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with GP64 viral secretion signal and C-terminal eGFP-HIS6DepositorInsertNcoI-GP64ss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
UseTagseGFP-HIS6ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF HA-TSAdwt
Plasmid#84517PurposeMammalian expression of TSAdDepositorInsertTSAd (SH2D2A Human)
UseTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
1161F
Plasmid#183138PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry 14-3-3σ
Plasmid#111881PurposeMode #2 phosphosite expression (split mCherry, using 14-3-3σ binding domain)DepositorTypeEmpty backboneUseTagsSplit mCherry system: 14-3-3σ fused to C-terminal…ExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB1975 (pUC19-PTEF1-PPGK1)
Plasmid#73296PurposeExample plasmid containing a bidirectional promoterDepositorInsertpTEF1-pPGK1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
CP1041-ABE8e-dimer
Plasmid#138499PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1041)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCellFree_G03 HDAC1
Plasmid#67101PurposeStandard for cell-free expression benchmarking.DepositorInsertHDAC1 (HDAC1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX Aps-SH2
Plasmid#46404DepositorInsertAps (SH2B2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 404-523PromoterTacAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGWB452
Plasmid#74834PurposeGateway cloning compatible binary vector for N-terminal fusion with G3GFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pOCC121
Plasmid#118893Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3CDepositorInsertNcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and MBP, cleavab…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB3050(gRNA XII-5)
Plasmid#73292PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AtUBQ10 (GB2478)
Plasmid#160594PurposeUBQ10 promoter from A. thaliana for constitutive expression in plant systems.DepositorInsertAtUBQ10
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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Zebrafish-gRNA-0008
Plasmid#42248PurposegRNA targeted to zebrafish gene tia1lDepositorInsertgRNA-tia1l (tia1l Zebrafish)
UseCRISPR; Zebrafish expressionTagsExpressionMutationPromoterT7Available sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGWB526
Plasmid#74868PurposeGateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pOCC70
Plasmid#118878Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
UseTagsmGFP, cleavable with TEV protease; HIS6, cleavabl…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_Myt1
Plasmid#64845PurposeA lentiviral vector for the expression of mouse Myt1 under doxycyline controlDepositorInsertMyt1 (Myt1 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
SaKKH-ABE8e-dimer
Plasmid#138503PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-nSaCas9 (KKH)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 NFKB1
Plasmid#67126PurposeStandard for cell-free expression benchmarking.DepositorInsertNFKB1 (NFKB1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSC44
Plasmid#104818PurposeCRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from AtUBQ10 promoter.DepositorInsertGlyma.04g057400
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-GAP-ACS1
Plasmid#126712Purposeyeast plasmid complementing ACS1 from Komagataella phaffiiDepositorInsertacs1
UseTags6xHis,MycExpressionYeastMutationPromoterGAPAvailable sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
LbABE8e-dimer
Plasmid#138505PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-dLbCas12a
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK233 (AAVS1 CMV-AtAFB2)
Plasmid#140537PurposeAAVS1 CMV-AtAFB2DepositorInsertCMV-AtAFB2
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRger3-TS-EYFP
Plasmid#127241PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.DepositorInsertChRger3-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianMutationPromoterCamKIIaAvailable sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CTTN
Plasmid#67100PurposeStandard for cell-free expression benchmarking.DepositorInsertCTTN (CTTN Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-Amrose v3
Plasmid#79539PurposepRSETa (Invitrogen) plasmid expressing Amrose variant 3DepositorInsertAmrose v3
UseTags6xHis, T7 epitope, and Xpress tagExpressionBacterialMutationPromoterT7Available sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIII-Neo-E2Crimson
Plasmid#153425PurposeRetroviral (MSCV) expression of E2-Crimson fluorescent proteinDepositorInsertE2-Crimson
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB3041(gRNA X-3)
Plasmid#73283PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-3DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIEG3-NFAT2
Plasmid#40351DepositorInsertNFAT2 (NFATC1 Mouse)
UseRetroviralTagsIRES eGFPExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_SCP1 vector_blocking 3
Plasmid#99318PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 DDX5
Plasmid#67111PurposeStandard for cell-free expression benchmarking.DepositorInsertDDX5 (DDX5 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat4-SH2
Plasmid#46514DepositorInsertStat4 (STAT4 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 133-678PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBICI_2_strong
Plasmid#155168Purposesee pBICI_strong: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syk(C)-SH2
Plasmid#46519DepositorInsertSyk(C) (SYK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 149-263PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB3047(gRNA XII-1)
Plasmid#73289PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-1DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX Slap-SH2
Plasmid#46500DepositorInsertSlap (SLA Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 61-221PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
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EK0545 SFFV-mCherry-SGc(GAPDH.s1)cSG-fs(EGFP)-(3xMS2) (FLP-IN)
Plasmid#191177PurposeMammalian expression of EK0493 with frameshift mutation (EGFP is frameshifted).DepositorInsertmCherry:GAPDH.s1:EGFP (frameshifted)
UseTagsExpressionMammalianMutationEGFP is frameshiftedPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
IBMc097
Plasmid#161945PurposeContains Type IIS restriction site free elements for IBM staging vectors. Staging plasmid for IBM on mCherryDepositorInsertpSB1C3(BsmBI-)-BsaI-mCherry*(27-218)-BsaI
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-1+t
Plasmid#48147PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
EK0387 SFFV-mTagBFP2-SGc(Bdnf.s1)cSG-mCherry (FLP-IN)
Plasmid#191157PurposeExpression of a sensor for murine Bdnf 3' UTR in mammalian cells; mTagBFP2 marker, mCherry output; 90 bp longDepositorInsertmTagBFP2:Bdnf.s1:mCherry
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only