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Showing: 7201 - 7250 of 13635 results
  1. pLP-hSox2-ires-gfp

    Plasmid
    #49391
    Purpose
    mammalian bicistronic expression of human Sox2 and EGFP
    Depositor
    Insert
    Sox2 (SOX2 Human)
    Use
    Cre/Lox; Acceptor vector
    Tags
    6xHN, EGFP, and IRES
    Expression
    Mammalian
    Mutation
    TGA (stop) to CCA (Pro) at nt 1027 with respect t…
    Promoter
    CMV
    Available since
    Dec. 2, 2013
    Availability
    Academic Institutions and Nonprofits only
  2. AP1297‐1

    Plasmid
    #87247
    Purpose
    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
    Depositor
    Insert
    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
    Use
    Tags
    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
  3. pZA48

    Plasmid
    #158526
    Purpose
    In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V/L17E-eGFP. Km plant selection marker.
    Depositor
    Insert
    ZAR1-eGFP
    Use
    Tags
    eGFP
    Expression
    Plant
    Mutation
    L17E, D481V
    Promoter
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pN256

    Plasmid
    #188472
    Purpose
    scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP
    Depositor
    Insert
    eGFP-WPRE-DAISY-4T
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pGS1222

    Plasmid
    #63791
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Twin-Strep tag followed by URA3 selection marker
    Insert
    eGFP-Twin-Strep tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eGFP-Twin-Strep tag
    Expression
    Mutation
    Promoter
    none
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
  6. KAP3-FL

    Plasmid
    #51413
    Purpose
    expression of EGFP-tagged full length human KAP3B in mammalian cells
    Depositor
    Insert
    Kinesin-associated protein 3 (KIFAP3 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 13, 2014
    Availability
    Academic Institutions and Nonprofits only
  7. pNHEJ-RPG

    Plasmid
    #85931
    Purpose
    Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells
    Depositor
    Insert
    pPB-CMV-DsRed-polyA-CAG-NHEJ. Puro-T2A-EGFP
    Use
    Dual-reporter surrogate
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. L2-AA007

    Plasmid
    #185750
    Purpose
    Vector to introduce HygR and eGFP under control of p35S into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.
    Depositor
    Inserts
    HygR2
    eGFP
    Use
    Tags
    membrane localization tag Lti6b
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pSSA-RPG

    Plasmid
    #85932
    Purpose
    Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells
    Depositor
    Insert
    pPB-CMV-DsRed-polyA-CAG-SSA. Puro-T2A-EGFP
    Use
    Dual-reporter surrogate
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
  10. pDN-D2irTN2AG5kwh

    Plasmid
    #128253
    Purpose
    Mammalian linearizer gene circuit in a Flp-In expression system based on negative feedback. The hTetR and EGFP is separated by a P2A motif.
    Depositor
    Insert
    Beta Globin Intron::hTetR::P2A::EGFP
    Use
    Synthetic Biology; Flp-in expression vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV D2ir promoter
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. KAP3-CT

    Plasmid
    #51396
    Purpose
    expression of EGFP-tagged COOH-terminal AA 661–793 KAP3B in mammalian cells
    Depositor
    Insert
    Kinesin-associated protein 3 (KIFAP3 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
  12. pOCC93

    Plasmid
    #118911
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with GP64 viral secretion signal and C-terminal eGFP-HIS6
    Insert
    NcoI-GP64ss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    Use
    Tags
    eGFP-HIS6
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
  13. L2-AA012

    Plasmid
    #185751
    Purpose
    Vector to introduce eGFP under control of pAaEF1a into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.
    Depositor
    Inserts
    HygR2
    eGFP
    Use
    Tags
    membrane localization tag Lti6b
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  14. pOCC92

    Plasmid
    #118910
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal eGFP-HIS6
    Insert
    NcoI-HBMss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    Use
    Tags
    eGFP-HIS6
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
  15. pOCC6

    Plasmid
    #118864
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal EGFP
    Insert
    NcoI-HIS6-3C-EGFP-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease, EGFP
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
  16. pHRIG-S6K

    Plasmid
    #59446
    Purpose
    lentiviral expression of S6K and EGFP
    Depositor
    Insert
    S6K (Rps6kb1 Rat)
    Use
    Lentiviral
    Tags
    IRES-EGFP
    Expression
    Mammalian
    Mutation
    S6K starts at aa24 and contains 7 extra amino aci…
    Promoter
    CMV
    Available since
    Oct. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 7201 - 7250 of 13635 results