We narrowed to 18,985 results for: INO

1. pcDNA3.1-HA-CDK2

   Plasmid

   #172613

   Purpose

   Expresses HA-tagged CDK2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK2 (CDK2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ai66(RCRL-tdT) targeting vector

   Plasmid

   #61578

   Purpose

   Target a Dre and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   CAG-RSR-LSL-tdTomato
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-nEF-Con/Fon/Von eYFP

   Plasmid

   #137164

   Purpose

   Intersectional viral expression of EYFP in cells expressing Cre AND Flp AND Vcre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV Retrograde and AAV8

   Insert

   3x-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   nEF

   Available since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pscALPSblasti-TMPRSS2 Blasti

   Plasmid

   #158088

   Purpose

   Expresses TMPRSS2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-nEF-Con/Fon-iC++-EYFP

   Plasmid

   #137155

   Purpose

   Intersectional viral expression of iC++-EYFP in cells expressing both Cre AND Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Fon-iC++-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   nEF

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV pTRE-FLEX-EGFP-WPRE-bGHpA

   Plasmid

   #65449

   Purpose

   Can be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetO

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSCAR_sgRNA_puro-mKate-lox5171

   Plasmid

   #162077

   Purpose

   3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLKO.1 puro CXCR4 siRNA-1

   Plasmid

   #12271

   Depositor

   Bob Weinberg

   Article

   Orimo et al Cell. 2005 May 6. 121(3):335-48.

   Insert

   CXCR4 siRNA (CXCR4 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHDM-SARS-CoV-2 Δ18

   Plasmid

   #164434

   Purpose

   Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   SARS-CoV-2 Spike truncated to remove 18 amino acids (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BII-BCA-Prom-ASCL2

    Plasmid

    #133394

    Purpose

    Piggybac vector for heterologous promoter reporter assay of human ASCL2 promoter

    Depositor

    Blair Madison

    Article

    Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

    Insert

    destabilized tdTomato-NLS and destabilized NLS-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free) drives expressi…

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-Ef1a-Con/Fon-sRGECO

    Plasmid

    #137126

    Purpose

    Intersectional viral expression of sRGECO in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-sRGECO
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    E217D

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-Ef1a-Coff/Fon-GCaMP6M

    Plasmid

    #137121

    Purpose

    Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-GCaMP6M
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFastbac-Dual-StrepII-BARD1

    Plasmid

    #137166

    Purpose

    Expresses human StrepII-tagged BARD1 for purification from baculovirus/insect cell system

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Insert

    BARD1 (BARD1 Human)
    

    Use

    Tags

    StrepII

    Expression

    Insect

    Mutation

    fully synthetic, codon optimized for insect expre…

    Promoter

    p10

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-AMPH1-BAR-mCherry

    Plasmid

    #85130

    Purpose

    BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry (AMPH Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLEX303-ARF6-mNeonGreen

    Plasmid

    #162027

    Purpose

    Expression of tagged ARF WT

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    ARF6 (ARF6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCS2+-N-PAS2-GAF-PHYB-MCherry-CAAX

    Plasmid

    #154910

    Purpose

    1-621 amino acids of Arabidopsis PHYB (Gene ID: 816394), tagged with mCherry fluorescence protein and the CAAX membrane moiety. Mammalian codon optimised.

    Depositor

    Jonathan Clarke, Clare Buckley

    Article

    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

    Insert

    Phytochrome B (PHYB Mustard Weed, Synthetic)
    

    Use

    Xenopus/avian/zebrafish

    Tags

    CAAX membrane moiety and mCherry

    Expression

    Mammalian

    Mutation

    1-621 amino acids of Arabidopsis PHYB

    Promoter

    CMV

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-Ef1a-Coff/Fon-BFP

    Plasmid

    #137131

    Purpose

    Intersectional viral expression of BFP in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-BFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. JWW-1 human chimeric antibody

    Plasmid

    #66748

    Purpose

    Expresses a rat/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 17-36. JWW-1 works in ELISA/WB/HPV Neutralization assay.

    Depositor

    Richard Roden

    Article

    Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

    Inserts

    JWW-1 Heavy Chain Gamma
    JWW-1 Light Chain Kappa
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    First 3 amino acids of rat light chain constant r…

    Promoter

    mEF1 and rEF1

    Available since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-Ef1a-Con/Fon-BFP

    Plasmid

    #137129

    Purpose

    Intersectional viral expression of BFP in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-BFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS D614 soluble Spike

    Plasmid

    #164652

    Purpose

    Expresses SARS-CoV-2 soluble Spike protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 soluble spike (S SARS-CoV-2)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

21. GFP-ataxin-3 C14A

    Plasmid

    #89977

    Purpose

    Expresses catalytic inactive GFP-tagged ataxin-3

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 14 to Alanine

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-Ef1a-Coff/Fon-GCaMP6F

    Plasmid

    #137124

    Purpose

    Intersectional viral expression of GCaMP6F in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-GCaMP6F
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLEX303-ARF6-T157N-mNeonGreen

    Plasmid

    #162028

    Purpose

    Fast cycling ARF mutant

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    ARF6 (ARF6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    T151N

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDEST17-ALDH1A3

    Plasmid

    #189750

    Purpose

    The construct was used to express recombinant ALDH1A3 in E.Coli. The purified protein was used for activity assay.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    ALDH1A3 (ALDH1A3 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CRY2-INPP5E(D556A)

    Plasmid

    #79564

    Purpose

    expression of CRY2PHR fused to catalytically dead INPP5E

    Depositor

    Olof Idevall-Hagren, Pietro De Camilli

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    INPP5E (INPP5E Human)
    

    Use

    Tags

    CRY2 (photolyase homology region domain of Arabid…

    Expression

    Mammalian

    Mutation

    C-terminal region, aa 214_644, C641A mutation to …

    Promoter

    CMV

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pMSCV-MAP3K2

    Plasmid

    #85652

    Purpose

    retroviral expression of human MAP3K2

    Depositor

    Julien Sage

    Article

    Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

    Insert

    MAP3K2 (MAP3K2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti PGK Puro GFP-GABARAPL1 G116

    Plasmid

    #123243

    Purpose

    Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116. For lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deleted amino acid 117. Stop codon after G116

    Promoter

    PGK

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-ITR-PytR(PyOtR)-mCherry

    Plasmid

    #204870

    Purpose

    Expresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAV

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

    Insert

    M. mazei pyrrolysyl tRNA mutant "PyOtR"
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    U25C; A3G, A4C, A5G, C6G, G63C, T65G, T66C

    Promoter

    U6

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPPI4-Strep-SNAP-CTLA-4-His6

    Plasmid

    #223583

    Purpose

    Protein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tag from mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    Tags

    His6 and Twin-Strep, SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCS2+-Y276H PHYB-CAAX

    Plasmid

    #154911

    Purpose

    1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.

    Depositor

    Jonathan Clarke, Clare Buckley

    Article

    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

    Insert

    Phytochrome B (PHYB Mustard Weed)
    

    Use

    Xenopus/avian/zebrafish

    Tags

    CAAX membrane moiety and mCherry

    Expression

    Mammalian

    Mutation

    1-621 amino acids of Arabidopsis PHYB, Tyrosine r…

    Promoter

    CMV

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TRIPZ-bioGEF-SUMO2nc-PURO

    Plasmid

    #208043

    Purpose

    Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    SUMO2nc (SUMO2 Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Expression

    Mutation

    Q90P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-Ef1a-Con/Foff 2.0-mCherry

    Plasmid

    #137133

    Purpose

    Intersectional viral expression of mCherry in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLEX301-RFP-GGA3 NGAT

    Plasmid

    #162031

    Purpose

    Expresses NGAT sequence of GGA3

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    GGA3 NGAT (GGA3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA8-Flag-LARS(1-976aa)

    Plasmid

    #139689

    Purpose

    Expresses N-teminal Flag tagged aa 1-976 of LARS1

    Depositor

    Jie Chen

    Article

    Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

    Insert

    LARS1 (LARS1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLEX304-GFP-v1 IQSEC1 WT

    Plasmid

    #162016

    Purpose

    Expression of IQSEC1 splice variant 1

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    IQSEC1 (IQSEC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLEX304-GFP-v2 IQSEC1 WT

    Plasmid

    #162017

    Purpose

    Expression of IQSEC1 splice variant 2

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    IQSEC1 (IQSEC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-B103 (LM2963)

    Plasmid

    #208960

    Purpose

    A variant CE1 construct with B103 DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-B103-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-PolBeta (JO574)

    Plasmid

    #208953

    Purpose

    A variant CE1 construct with human DNA polymerase beta, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-PolBeta-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-TaqStoffel (JO595)

    Plasmid

    #208952

    Purpose

    A variant CE1 construct with Taq Stoffel DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-TaqStoffel-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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40. pCDF1-WT_RASA2-EF1-Puro

    Plasmid

    #114096

    Purpose

    Express RASA2 in mamalian cells

    Depositor

    Yardena Samuels

    Article

    Arafeh et al Nat Genet. 2015 Dec;47(12):1408-10. doi:

    Insert

    RASA2 (RASA2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCLXSN(GFP)-HA-Slc1a5 (mouse)

    Plasmid

    #71458

    Purpose

    inducible expression fo Slc1a5 in mammalian cells

    Depositor

    Shao-Cong Sun

    Article

    Nakaya et al Immunity. 2014 May 15;40(5):692-705. doi

    Insert

    Slc1a5 (Slc1a5 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    A85P, L125F and V384A mutations compared to GenBa…

    Promoter

    CMV

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

42. pcDNA3.1 SARS-CoV-2 S D614G-trunc

    Plasmid

    #158077

    Purpose

    Expresses SARS-CoV-2 spike protein with a D614G truncated mutation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    Available since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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43. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

    Plasmid

    #208944

    Purpose

    A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pKLV-EF1a-BFPCDK6-W

    Plasmid

    #208756

    Purpose

    Lentiviral vector expressing BFP fused with CDK6 cDNA

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    CDK6 (CDK6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pEMS1424

    Plasmid

    #29220

    Purpose

    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple155-EGFP-NLS
    

    Use

    High copy number homologous recombination plasmid…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MiniPromoter Ple155 from the human PCP2 gene

    Available since

    Nov. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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46. pEMS2242

    Plasmid

    #111897

    Purpose

    ssAAV genome with Ple321 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple321 (TUBB3 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple321 (TUBB3 MiniPromoter)

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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47. pcDNA3-GFP-IMP2-2_antisense-control

    Plasmid

    #42174

    Depositor

    Alexandra Kiemer

    Article

    Kessler et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Insulin-like growth factor 2 mRNA binding protein 2-2 antisense control (IGF2BP2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

48. pENTR1A-HA-Jag1

    Plasmid

    #46052

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Jag1 (Jag1 Rat)
    

    Use

    Entry vector

    Tags

    HA tag

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

49. pLEX304-GFP-v3 IQSEC1 WT

    Plasmid

    #162018

    Purpose

    Expression of IQSEC1 splice variant 3

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    IQSEC1 (IQSEC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pLEX304-GFP-v4 IQSEC1 WT

    Plasmid

    #162019

    Purpose

    Expression of IQSEC1 splice variant 4

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    IQSEC1 (IQSEC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge