We narrowed to 23,099 results for: crispr

1. BPK2013-AkCas12b

   Plasmid

   #121950

   Purpose

   E. coli expression, protein purification

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AkCas12b
   

   Use

   Tags

   10xHis tag and 3xHA tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHAGE-IRES-puro-NLS-dHgm4Cas13b-EGFP-NLS

   Plasmid

   #191360

   Purpose

   Overexpression of NLS-dHgm4Cas13b-EGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-dHgm4Cas13b-EGFP-NLS
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

   Plasmid

   #133340

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_gcrA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. dCas12j3 KRAB

   Plasmid

   #188507

   Purpose

   Expresses FLAG tagged dCas12j3 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12j3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D413A

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-3a-His-MBP-TEV-VC_Cas1_WT

   Plasmid

   #120364

   Purpose

   Expresses wildtype V-C Cas1

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Insert

   VC_Cas1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-3a-His-MBP-TEV-VD_Cas1_WT

   Plasmid

   #120365

   Purpose

   Expresses wildtype V-D Cas1

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Insert

   VD_Cas1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFETCh_MIXL1

   Plasmid

   #86280

   Purpose

   Donor vector for 3' FLAG tag of human MIXL1

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   MIXL1 homology arms (MIXL1 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pM116: CasRx VEGFA presgRNA

   Plasmid

   #166868

   Purpose

   U6-driven expression of human VEGFA targeting presgRNA compatible with CasRx.

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Insert

   Human VEGFA targeting presgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

   Plasmid

   #133334

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_ctrA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFETCh_TEAD3

    Plasmid

    #86281

    Purpose

    Donor vector for 3' FLAG tag of human TEAD3

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    TEAD3 homology arms (TEAD3 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro

    Plasmid

    #192681

    Purpose

    Lentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Mouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pICSL11023

    Plasmid

    #117542

    Purpose

    Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S_OMEGA(pICH51288)

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFETCh_NCoA2

    Plasmid

    #86283

    Purpose

    Donor vector for 3' FLAG tag of human NCoA2

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    NCoA2 homology arms (NCOA2 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFETCh_NR2F6

    Plasmid

    #86284

    Purpose

    Donor vector for 3' FLAG tag of human NR2F6

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    NR2F6 homology arms (NR2F6 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDIV490

    Plasmid

    #177698

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RHR2p (Debaryomyces hansenii ) and TDH3p (Candida…

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. px458_2A_GFP_sgRNA_PRKRA

    Plasmid

    #106109

    Purpose

    CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting PRKRA

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    gRNA targeting PRKRA (PRKRA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKDsgRNA-rpsL

    Plasmid

    #89953

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rpsL.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    rpsL gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEJS507-pCDest2-noAcr-mTagBFP2-IRES

    Plasmid

    #85748

    Purpose

    Expresses mTagBFP2 only; used as a control

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    mTagBFP2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-BsCas12b-2AeGFP

    Plasmid

    #121948

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    BsCas12b
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMiniT:myc-GluA1 TKIT donor

    Plasmid

    #169419

    Purpose

    Donor DNA for TKIT knockin of Myc to GluA1 in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Myc (Myc Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPPC020.306

    Plasmid

    #171148

    Purpose

    Expression of J3-BBa_J23117-mRFP and J306 scRNA on pBBR1-GmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    J306 scRNA
    J3-BBa_J23117-mRFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 and J3-BBa_J23117

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFETCh_MLX

    Plasmid

    #86277

    Purpose

    Donor vector for 3' FLAG tag of human MLX

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    MLX homology arms (MLX Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUb LwaCas13a + LwaCas13a Guide RNA

    Plasmid

    #176307

    Purpose

    Expresses LwaCas13a and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    LwaCas13a
    

    Use

    Tags

    HA tag

    Expression

    Insect

    Mutation

    E656G, I1060V, D1076G- please see depositor comme…

    Promoter

    Ubi-p63e

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBbdCas9S_Psyn-sgRNAflaB

    Plasmid

    #149642

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi flaB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAflaB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJK526

    Plasmid

    #155387

    Purpose

    PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA4 in dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJK413

    Plasmid

    #155372

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pM215A

    Plasmid

    #173217

    Purpose

    Expresses ScFV-GCN4-sFGFP10M2-GB1

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    ScFV-GCN4-sfGFP10M2-GB1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pM214

    Plasmid

    #173216

    Purpose

    Expresses MCP-mCherry-GFP11-GB1

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    MCP-mCherry-GFP11-GB1
    

    Use

    CRISPR and Lentiviral

    Tags

    HA and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBbdCas9S_Psyn-sgRNAmreB

    Plasmid

    #149653

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-AmCas12b-2AeGFP

    Plasmid

    #121947

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AmCas12b
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCF204

    Plasmid

    #121656

    Purpose

    U6-sgRNA EFS-Cas9-wt-P2A-Puro

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    Cas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEPOR1CB0112

    Plasmid

    #117548

    Purpose

    Level 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plants

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S+5'UTR OMEGA (pICH51288)

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA

    Plasmid

    #133344

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_cpaA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    constitutive

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFETCh_ZGPAT

    Plasmid

    #86276

    Purpose

    Donor vector for 3' FLAG tag of human ZGPAT

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZGPAT homology arms (ZGPAT Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pM117: VEGFA array presgRNA

    Plasmid

    #166869

    Purpose

    U6-driven expression of human VEGFA targeting array presgRNA compatible with CasRx.

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Insert

    Human VEGFA targeting array presgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJK429

    Plasmid

    #155376

    Purpose

    PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pKDsgRNA-malG

    Plasmid

    #89951

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    malG gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFETCh_ZBTB7B

    Plasmid

    #135730

    Purpose

    Donor vector for 3' FLAG tag of human ZBTB7B

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZBTB7B Homology arms (ZBTB7B Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. dSp-VP64-p65(150-261)-RTA(125-190)

    Plasmid

    #99674

    Purpose

    Expresses dSp Cas9 fused to combination of truncated activation domains

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    Tags

    VP64-P65(151-261)-RTA(125-190)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Promoter

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pKDsgRNA-hisG

    Plasmid

    #89954

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    hisG gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCF226

    Plasmid

    #121667

    Purpose

    EFS-Cas9-wt-P2A-Puro

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    cas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a core promoter

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pKDsgRNA-trmI

    Plasmid

    #89955

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    trmI gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pENTR-R4R3-stop

    Plasmid

    #113745

    Purpose

    Contains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    stop cassette
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Added 3 stop codons to the original sequence; see…

    Promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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44. pET28b-NmCas9-Flag H588A

    Plasmid

    #109032

    Purpose

    H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Use

    Tags

    Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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45. pFETCh_SOX5

    Plasmid

    #86292

    Purpose

    Donor vector for 3' FLAG tag of human SOX5

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    SOX5 homology arms (SOX5 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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46. pFETCh_ZNF644

    Plasmid

    #86264

    Purpose

    Donor vector for 3' FLAG tag of human ZNF644

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZNF644 homology arms (ZNF644 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pKDsgRNA-xylA

    Plasmid

    #89964

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    xylA gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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48. pKDsgRNA-lpoB

    Plasmid

    #89959

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    lpoB gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pU6_(GLuc)_INT[GFPApt]

    Plasmid

    #68428

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing the GFP aptamer, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing the GFP aptamer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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50. pU6_(GLuc)_INT[3xK-T]

    Plasmid

    #68429

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing three kink-turns, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing three kink-turns
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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