164,946 results

1. pEGFP-N1 Full length Importin beta

   Plasmid

   #106941

   Purpose

   Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

   Depositor

   Patrizia Lavia

   Article

   Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

   Insert

   human Importin beta-1 (KPNB1) (KPNB1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nme2Cas9_AAV

   Plasmid

   #119924

   Purpose

   All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   Nme2Cas9
   

   Use

   AAV

   Tags

   NLS-3xHA-NLS and NLS-NLS

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. PIK3CA - Exon20 H1047R

   Plasmid

   #16639

   Depositor

   Bert Vogelstein

   Article

   Samuels et al Science. 2004 Apr 23. 304(5670):554.

   Insert

   PIK3CA H1047R (PIK3CA Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   H1047R

   Available Since

   March 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. hSOD1-FLAG

   Plasmid

   #182922

   Purpose

   Mammalian expression plasmid for human WT SOD1

   Depositor

   Philip Eaton

   Article

   Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

   Insert

   [Cu-Zn] Superoxide dismutase 1 (SOD1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX208 CMV sTurboID (C)-HA-FRB-ERM

   Plasmid

   #153007

   Purpose

   Expresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membrane

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID (C) (G74)-ERM (Cb5)
   

   Use

   Lentiviral

   Tags

   FRB and HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRRL_SRSF2_WT_mCherry

   Plasmid

   #84020

   Purpose

   expression of SRSF2 in mammalian cells

   Depositor

   Robert Bradley

   Article

   Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

   Insert

   SRSF2 (SRSF2 Human)
   

   Use

   Lentiviral

   Tags

   2TA-mCherry and Flag

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEMTB-mScarlet3-H (aka mYongHong)

   Plasmid

   #239407

   Purpose

   Expresses EMTB-mScarlet3-H in mammalian cells

   Depositor

   Zhifei Fu

   Article

   Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

   Insert

   EMTB (MAP7 Human)
   

   Tags

   mScarlet3-H (aka mYongHong)

   Expression

   Mammalian

   Mutation

   M163H in mScarlet3

   Promoter

   CMV

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSH-EFIRES-P-AtAFB2-mCherry

   Plasmid

   #129716

   Purpose

   AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   AFB2 (AFB2 Mustard Weed)
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   EF1a

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCBC-MT3T4

   Plasmid

   #50595

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T3, gRNA scaffold, U6-26t plus, OsU3p, T4
   

   Use

   CRISPR; Pcr template

   Expression

   Plant

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-Fab_8D3_2_H-H6

    Plasmid

    #176075

    Purpose

    Express His6 tagged heavy chain of Fab_8D3_2 in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    His6 tagged heavy chain of Fab_8D3_2
    

    Tags

    His tag

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-Fab_8D3_2_L

    Plasmid

    #176076

    Purpose

    Express light chain of Fab_8D3_2 in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    Light chain of Fab_8D3_2
    

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST-CMV GFP-LC3B WT

    Plasmid

    #123114

    Purpose

    Expresses EGFP-LC3B wild-type in mammalian cells. Fluorescent reporter for autophagosomes.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. AiP12558 - pAAV-AiE0390m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2558)

    Plasmid

    #208141

    Purpose

    AiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. KIF1A(1-393)-GCN4-2xmCherry-EF(C)

    Plasmid

    #61665

    Purpose

    Tandem mCherry-labeled, forced-dimeric, truncated kinesin-3 fused to EF Hand linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF1A(1-393)-GCN4-2xmCherry-EF(C) (KIF1A Human)
    

    Expression

    Mammalian

    Available Since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1a_MYC_P2A_Hygro_Barcode

    Plasmid

    #120461

    Purpose

    Barcoded lentiviral vector to express MYC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSFDUET-1_VNp-anti-6xHis mAb

    Plasmid

    #214952

    Purpose

    Bacterial expression of anti-6xHis mAb light and heavy chain VNp fusions.

    Depositor

    Dan Mulvihill

    Article

    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

    Inserts

    VNp-anti-6xHis mAb heavy chain
    VNp-anti-6xHis mAb light chain
    

    Tags

    VNp6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-dCas13-M3M14nes

    Plasmid

    #155367

    Purpose

    Targeted m6A RNA methylation in mammalian cells

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pN3-MGA

    Plasmid

    #107715

    Purpose

    Expression plasmid for 3xFLAG-tagged MGA

    Depositor

    Guntram Suske

    Article

    Stielow et al PLoS Genet. 2018 Jan 30;14(1):e1007193.

    Insert

    MGA (MGA Human)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pInducer20-Blast

    Plasmid

    #109334

    Purpose

    pInducer20 with added blasticidin resistance

    Depositor

    Jean Cook

    Article

    Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

    Type

    Empty backbone

    Use

    Lentiviral; Inducible expression

    Expression

    Mammalian

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. CYFIP1.mCherry

    Plasmid

    #122051

    Purpose

    Expresses human CYFIP1 in mammalian cells.

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP1 (CYFIP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA-SET-FLAG-HA

    Plasmid

    #24998

    Depositor

    Judy Lieberman

    Article

    Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

    Insert

    SET (SET Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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22. pATetO 6XHis T4 DNA Ligase

    Plasmid

    #206207

    Purpose

    Inducible expression of T4 DNA ligase

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

    Insert

    T4 DNA ligase
    

    Expression

    Bacterial

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pRK5_mCherry_P2AT2A_GFP-nb

    Plasmid

    #238232

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLenti CMV Neo DEST (705-1)

    Plasmid

    #17392

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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25. pPAmCherry1-C1

    Plasmid

    #31929

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Expression

    Mammalian

    Mutation

    E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

    Promoter

    CMV

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)

    Plasmid

    #140892

    Purpose

    Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.

    Depositor

    Yasushi Okamura

    Article

    Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1

    Insert

    CiDr-VSP L223F-mCherry (tpte, vsp Zebrafish, Ciona intestinalis)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    changed 223L to F in Dr-VSP (zebrafish TPTE)

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET His6 TEV LIC cloning vector (2B-T)

    Plasmid

    #29666

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6-TEV

    Expression

    Bacterial

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Px461-Cas9n-Trp53-sgRNA-beta

    Plasmid

    #88847

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

29. pU6-BbsI-chiRNA

    Plasmid

    #45946

    Purpose

    Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

    Depositor

    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

    Article

    Gratz et al Genetics. 2013 May 24.

    Insert

    U6-BbsI-chiRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    Dm-snRNA:U6:96Ab

    Available Since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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30. pDEST47-SAR1-GFP

    Plasmid

    #67409

    Purpose

    Mammalian expression of hSar1 with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SAR1 (SAR1A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

31. Px461-Cas9n-Trp53-sgRNA-alpha

    Plasmid

    #88846

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

32. pcDNA3-FLAG-Luciferase

    Plasmid

    #58792

    Purpose

    Express FLAG-tagged Luciferase in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    firefly Luciferase
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pCdrA-gfp(ASV)C

    Plasmid

    #111615

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(ASV)-T0-T1
    

    Expression

    Bacterial

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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34. pMRX-IZ-ANXA7-mRuby3

    Plasmid

    #184893

    Purpose

    Stable expression of ANXA7-mRuby3 (Annexin A7-mRuby3) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    ANXA7 (ANXA7 Human)
    

    Tags

    mRuby3

    Expression

    Mammalian

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. ancBE4-SpRY

    Plasmid

    #226855

    Purpose

    Expresses ancBE4max-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ancBE4max-SpRY
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLVH-EF1a-GFP-P2A-MYC

    Plasmid

    #130695

    Purpose

    Lentiviral plasmids encoding human MYC with co-expressoin of GFP mediated by P2A.

    Depositor

    Kejin Hu

    Article

    Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

    Insert

    human MYC (MYC Human)
    

    Use

    Lentiviral

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pUGI-NLS

    Plasmid

    #101091

    Purpose

    Expresses UGI in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

    Insert

    UGI
    

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pNitET-SacB-kan

    Plasmid

    #107692

    Purpose

    Expression of the phage Che9c RecET system for Recombineering in mycobacteria

    Depositor

    Kenan Murphy

    Article

    Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

    Inserts

    Che9 phage RecET
    PNit2-NitR
    SacRB
    

    Expression

    Bacterial

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mCherry2-N1

    Plasmid

    #54517

    Purpose

    Localization: N1 Cloning Vector, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    mCherry2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Lenti-LucOS

    Plasmid

    #22777

    Depositor

    Tyler Jacks

    Insert

    OVA-SIY
    

    Use

    Cre/Lox and Lentiviral

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge