We narrowed to 6,511 results for: itch
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Plasmid#178574PurposeLentiviral vector for CamKIIα::Opto-vTrapDepositorInsertCRY2PHR-P2A-CIBN-mCherry-VAMP2
UseLentiviralTagsExpressionMutationPromoterCaMKIIaAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CIBN-GFP-TMD
Plasmid#79571Purposeexpression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)DepositorInsertsyntaxin 1A (Stx1a Mouse)
UseTagsCIBN and EGFPExpressionMammalianMutationaa 249_288 (C-terminal region (polybasic stretch …PromoterCMVAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax
Plasmid#173874PurposeMammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)DepositorInsertmCherry-NES-SspB-C3Gcat-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-CAAX
Plasmid#51567PurposeConstitutively express PM (Plasma membrane) localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsCAAX and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV_pSyn_Mito-LOV-Turbo-V5
Plasmid#199669Purposeexpresses Mito-LOV-Turbo-V5 in neurons, AAV vectorDepositorInsertLOV-Turbo
UseAAVTagsCOX4I1 mito targeting sequence and V5ExpressionMutationPromoterSynapsinAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
CALID
Plasmid#169708PurposeCre-activated light-inducible Dre recombinaseDepositorInserta double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
UseTagsExpressionMammalianMutationnonePromoterCAGAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mScarlet-H2BC11
Plasmid#207756PurposeDonor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Empty Target Control
Plasmid#127667PurposeEncodes for the CRY2-HA and mCherry proteins expressed from the EF1a promoterDepositorInsertCRY2-HA-2A-mCherry
UseTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CRY2Clust-FUSN-mut
Plasmid#200970Purposeinduction of phase-separated condensate mediated by FUSN-IDR mutantDepositorInsertFUSNmut
UseLentiviralTagsExpressionBacterial and MammalianMutation15 Tyr-to-Ser substitutionsPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2+PhyBΔCreC
Plasmid#131781PurposeMammalian, Zebrafish, or Xenopus expression of PhyBΔCreC, one half of the CreLite system.DepositorInsertPhyBCreC
UseCre/Lox; Zebrafish expressionTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX
Plasmid#188991PurposeA plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
UseTagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationF163L, K249R, G328S, G381V, G429D, D480GPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-GFAP-CRY2PHR-P2A-CIBN-mCherry-VAMP2
Plasmid#178575PurposeLentiviral vector for GFAP::Opto-vTrapDepositorInsertCRY2PHR-P2A-CIBN-mCherry-VAMP2
UseLentiviralTagsExpressionMutationPromoterGFAPAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPHR-iRFP713
Plasmid#103818PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-iRFP713 (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFX-mito-at pHluorin
Plasmid#174183PurposeExpress EYFP with mitochondria-targeting sequence in mammalian cellsDepositorInsertat pHluorin with mitchondria-targeting sequence
UseTagsthe mitochondria-targeting sequence of the cytoch…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
DHFR-GFP-th-NL1
Plasmid#173007PurposeFor light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.DepositorInsertDHFR-GFP-NL1
UseTagsDHFR and EGFPExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-CIBN-EGFP-KRasCT
Plasmid#178527PurposeExpresses CIBN-EGFP-KRasCT in mammalian cellsDepositorInsertCIBN-EGFP-KRasCT
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_Sensor_32B_LacZ
Plasmid#75007PurposeToehold switch sensor to detect Zika VirusDepositorInsertZIKV_sensor_32B_LacZ
UseTagsLacZExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-CreLite
Plasmid#131782PurposeMiddle entry vector (Tol2 kit) with bi-cistronic CreLite system components, PhyBΔCreC and PIF6CreN, linked by P2A.DepositorInsertPhyBCreC-P2A-PIF6CreN
UseCre/Lox; Gateway cloningTagsExpressionMutationPromoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cry2PHR.mCh.CofS3E
Plasmid#133619PurposeExpresses Cry2PHR - mCherry - CofilinS3E protein fusionDepositorInsertCry2PHR - mCherry - CofilinS3E
UseTagsExpressionMammalianMutationPromotercmvAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX
Plasmid#188992PurposeA plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
UseTagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationK109R, P245A, G328S, G381V, G429DPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
DHFR-GFP-th-GluA1
Plasmid#173008PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.DepositorInsertDHFR-GFP-th-GluA1
UseLentiviralTagsGFPExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
TIA1 RRM-YFP-SspBx6
Plasmid#103781Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertTIA1 RRM-YFP-SspBx6
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CreLite
Plasmid#131786PurposeLentiviral vector expressing CreLite, driven by CBh promoterDepositorInsertPhyBCreC-P2A-PIF6CreN
UseCre/Lox, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterCBhAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR
Plasmid#32483PurposeCre-dependent expression of ChR2 and PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP markerDepositorInsertChR2HA-2a-PSAML141F,Y115F:GlyR
UseAAV and Cre/Lox; Adeno-associated virus, flex swi…TagsChR2 2AExpressionMutationPromoterCAGAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pROF402
Plasmid#155334PurposeBinary vector for expression of FLuc controlled by the blue light-repressible systemDepositorInsertLB_P35Senhancer(-953 to -51)-(C120)5-PhCMVmin-FLuc-T35S_RB
UseLuciferase and Synthetic Biology; Eukaryotic expr…TagsExpressionMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
K401-Micro
Plasmid#122485PurposeComponent of optically-induced motor dimerization systemDepositorInsertHis tag MBP Kinesin 1-401 SspB micro
UseTagsH6-MBP-TEV and SspB microExpressionBacterialMutationKinesin amino acids 1-401PromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax
Plasmid#173868PurposeMammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)DepositorInsertmVenus-NES-SspB-C3Gcat-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax
Plasmid#173867PurposeMammalian expression of mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)DepositorInsertmVenus-NES-SspB-SOS2cat-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-LARG-DH-P2A-iLIDcaax
Plasmid#173863PurposeMammalian expression of mVenus-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)DepositorInsertmVenus-NES-SspB-LARG-DH-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-CDC10
Plasmid#51568PurposeConstitutively express bud neck localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsCDC10 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5
Plasmid#125236PurposeExpresses modified SPARK β2AR-NanoLuc transmembrane component in mammalian cellsDepositorInsertβ2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5
UseAAVTagsFlag, V5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX
Plasmid#188993PurposeA plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
UseTagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationD201G, A258T, K327R, G328S, G381V, G429D, T450APromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA
Plasmid#47454PurposeepiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.DepositorInsertCRY2PHR-NLS-SID4X
UseAAVTags2A_phiLOV2.1ExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax
Plasmid#173875PurposeMammalian expression of mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)DepositorInsertmVenus-NES-SspB-RGL2GEF-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax
Plasmid#173876PurposeMammalian expression of mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)DepositorInsertmCherry-NES-SspB-RGL2GEF-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
CRY2-mCherry-iTrkA
Plasmid#106168Purposemammalian expression of CRY2PHR (aa 1-498) fused to mCherry and intracellular domain of TrkA (aa 450-799)DepositorInsertCRY2PHR mCherry iTrkA (CRY2 Mustard Weed, Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hDcp2 E147Q, E148Q
Plasmid#72215Purposebacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)DepositorInsertDcp2 (DCP2 Human)
UseTagsHisExpressionBacterialMutationE147Q, E148QPromoterT7Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-Spc72
Plasmid#51582PurposeConstitutively express spindle pole body localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsSpc72 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HE-Venus-bPAC(F198Y)
Plasmid#165487PurposeXenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activityDepositorInsertVenus-bPAC(F198Y)-myc
UseCdna expression, xenopus oocyteTagsMyc and VenusExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-MCS-P2A-iLIDcaax
Plasmid#173864PurposeMammalian expression of mVenus-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)DepositorInsertmVenus-NES-SspB-MCS-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only