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Showing: 561 - 600 of 2179 results

  3. pACT2-μ3A

    Plasmid
    #198174
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:
    Insert
    AP-3 μ3A
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    silent substitution in codon 225
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198174-plasmid-map-sequence-id-387537

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  4. pACT2-μ3B

    Plasmid
    #198175
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:
    Insert
    AP-3 μ3B
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198175-plasmid-map-sequence-id-387536

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  9. Stx1a-12aa-mCer

    Plasmid
    #53236
    Purpose
    Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    Syntaxin 1A (Stx1a Rat)
    Use
    Tags
    12aa-linker and mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pBridge-tyrosinase.σ2

    Plasmid
    #197415
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-2 σ2
    Use
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    encodes R42G substitution, contains silent substi…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197415-plasmid-map-sequence-id-388893

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  19. pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.GPI

    Plasmid
    #196223
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV hSyn FLP-FRT iGluSnFR3 v857.GPI
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    GPI anchor, IgK-chain, and Myc epi tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    196223-plasmid-map-sequence-id-405486

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  20. pET-APOBEC1-YTHmut

    Plasmid
    #200159
    Purpose
    For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification
    Depositor
    Kate Meyer
    Article
    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882
    Insert
    APOBEC1-YTHmut
    Use
    Tags
    6xHis, HA, and MBP
    Expression
    Bacterial
    Mutation
    Codon optimized for E coli expression
    Promoter
    LacZ
    Available since
    June 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    200159-plasmid-map-sequence-id-396325

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  21. pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE

    Plasmid
    #104496
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has Service
    AAV PHP.eB, AAV Retrograde, and AAV1
    Insert
    jGCaMP7f
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only

  23. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10

    Plasmid
    #218864
    Purpose
    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Article
    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.
    Insert
    RSET-jGCaMP8s
    Use
    Tags
    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…
    Expression
    Insect
    Mutation
    S26M F286Y Q315H
    Promoter
    hsp70
    Available since
    May 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    218864-plasmid-map-sequence-id-433411

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  35. SnFR-gamma8

    Plasmid
    #165496
    Purpose
    Green fluorescent glutamate reporter fused to TARP gamma-8 for mammalian expression.
    Depositor
    Andrew Plested
    Article
    Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755
    Insert
    iGluSnFR extracellular domain fused to gamma-8 via NETO2 TM domain (Cacng8 Rat, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    165496-plasmid-map-sequence-id-327724

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  39. pGEX4T3-Stx3(1-265)

    Plasmid
    #100573
    Purpose
    Expresses cytoplasmic domain of Stx3(1-265) with a GST tag on the N-term.
    Depositor
    Thomas Weimbs
    Insert
    Stx3-1-265 (Stx3 Rat)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    No transmembrane domain, residues 4-264 present.
    Promoter
    tac
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    100573-plasmid-map-sequence-id-192872

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Showing: 561 - 600 of 2179 results