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Showing: 701 - 750 of 11666 results

  1. pcDNA3.1-3'TwStrep

    Plasmid
    #208618
    Purpose
    Empty vector for mammalian expression, insert Twin Strep tag using BamHI and AflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with Twin Strep tag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Twin Strep
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208618-plasmid-map-sequence-id-418314

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  4. pcDNA3.1-hUCP4-NE

    Plasmid
    #102362
    Purpose
    Overexpression of solute carrier family 25 member 27 in mammalian cells
    Depositor
    Shu Leong Ho
    Insert
    solute carrier family 25 member 27 (SLC25A27 Human)
    Use
    Tags
    NE
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    102362-plasmid-map-sequence-id-200644

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  11. NXPE1_pcDNA3.4-TOPO

    Plasmid
    #226841
    Purpose
    Expresses NXPE1 protein residues 60-547 in mammalian cells (HEK293T or expi293). x6 His tag and IL2 secretion signal included N terminal with GGGG linker to protein sequence.
    Depositor
    Bert Vogelstein
    Insert
    NXPE1 (NXPE1 Human)
    Use
    Tags
    x6 HIS and an IL2 secretion signal both N termina…
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells
    Promoter
    Available since
    Dec. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    226841-plasmid-map-sequence-id-456206

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  19. pcDNA3-PM-CaNAR2

    Plasmid
    #64730
    Purpose
    Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.
    Depositor
    Jin Zhang
    Article
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    Lyn-CaNAR2
    Use
    Tags
    Cerulean3, N-terminal targeting sequence from Lyn…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64730-plasmid-map-sequence-id-109348

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  21. pcDNA3-HA-14-3-3 epsilon

    Plasmid
    #13273
    Depositor
    Michael Yaffe
    Insert
    14-3-3 epsilon (YWHAE Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 7, 2006
    Availability
    Academic Institutions and Nonprofits only

  27. pcDNA3.3_SARS2_XBB.1.16

    Plasmid
    #201189
    Purpose
    expressing SARS-CoV-2 XBB.1.16 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (XBB.1.16) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E180V, T(K)478R on the basis of XBB.1.5
    Promoter
    CMV
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    201189-plasmid-map-sequence-id-393640

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  30. pcDNA3-StagRFP

    Plasmid
    #173014
    Purpose
    Rationally enhanced red fluorescent protein derived from TagRFP-T. For mammalian expression.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:
    Insert
    StagRFP
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    173014-plasmid-map-sequence-id-343313

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  35. pcDNA3.1 ShhN

    Plasmid
    #37680
    Depositor
    Philip Beachy
    Insert
    shh (Shh Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    soluble form (see comments)
    Promoter
    CMV
    Available since
    July 10, 2012
    Availability
    Academic Institutions and Nonprofits only
    37680-plasmid-map-sequence-id-159475

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  36. 4LNDDE488Q-pCDNA3.1(+)

    Plasmid
    #172228
    Purpose
    Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) in mammalian cells
    Depositor
    Joshua Rosenthal
    Article
    Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15
    Insert
    Lambda N peptide - human ADAR2 Deaminase Domain E488Q
    Use
    Tags
    6xHis and Flag
    Expression
    Mammalian
    Mutation
    changed the Glutamic acid 488 in the Deaminase Do…
    Promoter
    CMV
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    172228-plasmid-map-sequence-id-339581

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  50. pcDNA3-OPN-V5

    Plasmid
    #11617
    Depositor
    Steven Johnson
    Insert
    Osteopontin (SPP1 Human)
    Use
    Tags
    His and V5
    Expression
    Mammalian
    Mutation
    C9L and E160K
    Promoter
    CMV
    Available since
    July 18, 2007
    Availability
    Academic Institutions and Nonprofits only
Showing: 701 - 750 of 11666 results