We narrowed to 3,421 results for: ttl

1. pAct:dCas9-VPR

   Plasmid

   #78898

   Purpose

   Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   pActin (Drosophila)

   Available since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pENTR221-NR1H3

   Plasmid

   #79514

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   LXR Alpha (NR1H3 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. dCas9-CRY2Clust-BRD4ΔN

   Plasmid

   #200968

   Purpose

   induction of phase-separated condensate mediated by BRD4dN-IDR

   Depositor

   Jian Xu

   Article

   Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

   Insert

   BRD4dN
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. hDHFR

   Plasmid

   #184707

   Purpose

   bacterial expression of human DHFR

   Depositor

   Peter Wright

   Article

   Bhabha et al FEBS Lett. 2011 Nov 16;585(22):3528-32.

   Insert

   DHFR (DHFR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full

   Plasmid

   #79383

   Purpose

   Cas9 gRNA variant to recruit ms2 proteins

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   Zhang 2.0 + scaffold
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEN-L1-PJ23119-PplpT-sfGFP-TrrfB-BsaI-Scaf-L2

   Plasmid

   #196250

   Purpose

   Plasmid for cloning of a CRISPR-Cas9 guide RNA gene under promoter PJ23119

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   attL1-GlpT-scGFP-attL2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EGFP-NIK

   Plasmid

   #111197

   Purpose

   fluorescent fusion protein

   Depositor

   Johannes A. Schmid

   Article

   Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

   Insert

   NIK (MAP3K14 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TDP43 NOTAG2

   Plasmid

   #28207

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa86-414 only

   Promoter

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

9. mcherry-Myo 9b R1695M

   Plasmid

   #134958

   Purpose

   Expression of rat Myo 9b (Myr 5) in mammalian cells.GAP minus mutant.

   Depositor

   Martin Bähler

   Article

   Hemkemeyer et al J Biol Chem. 2021 Jan-Jun;296:100136. do

   Insert

   Myo 9b (rat) with 3' UTR. GAP minus mutant R1695M (Myo9b Rat)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR221-NFAT5

    Plasmid

    #79506

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NFAT5 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    NFAT5 (NFAT5 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TDP43 NOTAG6

    Plasmid

    #28209

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa1-314 only

    Promoter

    Available since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pEE491

    Plasmid

    #149292

    Purpose

    T-DNA encoding TRV2 with tRNA multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_tRNA_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJG85

    Plasmid

    #89281

    Purpose

    Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

    Depositor

    Daniel Voytas

    Article

    Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEE531

    Plasmid

    #149290

    Purpose

    T-DNA encoding TRV2 with spacer multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_Spacer_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR221-SMAD3

    Plasmid

    #79507

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human SMAD3 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    SMAD3 (SMAD3 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSUM-Kan-MCS2

    Plasmid

    #109289

    Purpose

    Expresses high levels of protein in mycobacteria

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    pSUM-kan-MCS1-gfp was modified to reorient the Ba…

    Promoter

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPSP6+t

    Plasmid

    #48149

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TDP43 NOTAG3

    Plasmid

    #28208

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa170-414 only

    Promoter

    Available since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pSPAsp-hp

    Plasmid

    #48122

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    predicted optimal signal peptide for Bacillus megaterium protein secretion
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEN41A-T38S38

    Plasmid

    #49524

    Purpose

    Ampicillin resistant; entry clone encoding reverse TetR (tetR38) and contains attL4 and attL1 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    constitutive TetR38
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Ptb38

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEN12A-P750

    Plasmid

    #49525

    Purpose

    Ampicillin resistant; entry clone containing PtetO-4C5G and attL1 and attL2 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    P750
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    P750

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSPYngK+-hp

    Plasmid

    #48121

    Purpose

    Shuttle vector E. coli/B.meg.; carrying the codon optimized sequence of the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    codon optimized signal peptide of YngK
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. EYFP-NIK

    Plasmid

    #111208

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

    Insert

    NIK (MAP3K14 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSFG-TFII-I-GFP beta

    Plasmid

    #22200

    Depositor

    Ananda Roy

    Article

    Hakre et al Mol Cell. 2006 Oct 20. 24(2):301-8.

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJOE5751.1

    Plasmid

    #135083

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

    Insert

    P-rhaP-BAD-His tag-eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPSP6

    Plasmid

    #48150

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMTTH+eGFP-bromo5(N140A)

    Plasmid

    #157806

    Purpose

    Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1 with N140A mutation

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    eGFP-bromo5(N140A)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    N140A

    Promoter

    pTAC

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Ef1a-QCP-3xBFP

    Plasmid

    #158206

    Purpose

    Qb coat protein fused to three repeats of the blue fluorescent protein under a Efla promoter.

    Depositor

    Roee Amit

    Article

    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

    Insert

    QCP under the Elfa promoter and three BFP tags
    

    Use

    Synthetic Biology

    Tags

    3xBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Elfa

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSPYngK-hp

    Plasmid

    #48118

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    signal peptide of YngK
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEE495

    Plasmid

    #149291

    Purpose

    T-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_Direct_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCheck2 Dest (R1-R2) #BGV129

    Plasmid

    #48955

    Purpose

    Destination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Luciferase; Gateway cloning vector

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pPK1E-1+t

    Plasmid

    #48147

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. M-ST1-VPR

    Plasmid

    #68498

    Purpose

    nuclease competent ST1-Cas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    ST-Cas9-VPR
    

    Use

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSUM-GroEL-gfp

    Plasmid

    #109286

    Purpose

    Expresses gfp in mycobacteria

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Insert

    GroEL-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GFP modified for expression in prokaryotes; inclu…

    Promoter

    GroEL

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSum-LacZ-gfp

    Plasmid

    #109285

    Purpose

    Expresses gfp in mycobacteria

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GFP modified for expression in prokaryotes; inclu…

    Promoter

    LacZalpha

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pPK1E-2+t

    Plasmid

    #48148

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSPPac-hp

    Plasmid

    #48123

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    signal peptide of Pac
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJOE6089.4

    Plasmid

    #135081

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

    Insert

    P-rhaP-BAD-eGFP-Strep-tag II
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSPNprM-hp

    Plasmid

    #48120

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein NprM under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    signal peptide of NprM
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSPVpr-hp

    Plasmid

    #48119

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Vpr under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    signal peptide of Vpr
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. TDP43 NOTAG11

    Plasmid

    #28210

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa193-274 only

    Promoter

    Available since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

42. pEF1-Pls3-sfGFP

    Plasmid

    #205750

    Purpose

    Expresses sfGFP under IPTG inducible promoter Pls3 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/High strength.

    Depositor

    Jerome Bonnet

    Article

    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

    Type

    Empty backbone

    Use

    Shuttle vector gram+ gram-

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pls3 (Phyperspank mutant, IPTG inducible)

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pEF1-Pls4-sfGFP

    Plasmid

    #205751

    Purpose

    Expresses sfGFP under IPTG inducible promoter Pls4 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. High strength.

    Depositor

    Jerome Bonnet

    Article

    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

    Type

    Empty backbone

    Use

    Shuttle vector gram+ gram-

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pls4 (Phyperspank mutant, IPTG inducible)

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. psiCHECK2_Rluc-Y0x-Fluc

    Plasmid

    #233614

    Purpose

    Expression of Rluc-Fluc reporter gene containing no Y

    Depositor

    Shintaro Iwasaki

    Article

    Matsuura-Suzuki et al Elife. 2022 Jun 8;11. pii: 72780. doi: 1

    Insert

    Rluc-Y0x-Fluc
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pKEK3235

    Plasmid

    #234107

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    sfGFP
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    S30R/Y39N/N105T/Y145F/I171V/A206V

    Promoter

    pJ23100-BsaI

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pKEK3220

    Plasmid

    #234099

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    sfGFP
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    S30R/Y39N/N105T/Y145F/I171V/A206V

    Promoter

    pJ23100-BsaI

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pKEK3237

    Plasmid

    #234091

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    sfGFP
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    S30R/Y39N/N105T/Y145F/I171V/A206V

    Promoter

    pJ23100-BsaI

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pKEK3400

    Plasmid

    #234084

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    sfGFP
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    S30R/Y39N/N105T/Y145F/I171V/A206V

    Promoter

    pJ23100-BsaI

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. psiCHECK2-1_Rluc-Y39x-Fluc

    Plasmid

    #233615

    Purpose

    Expression of Rluc-Fluc reporter gene containing 39xY

    Depositor

    Shintaro Iwasaki

    Article

    Matsuura-Suzuki et al Elife. 2022 Jun 8;11. pii: 72780. doi: 1

    Insert

    Rluc-Y39x-Fluc
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. acvr1l-VAAA-HA

    Plasmid

    #201905

    Purpose

    in vitro transcription of RNA encoding HA-tagged Acvr1l that contains 4 amino acid substitutions: T186V, S187A, S189A, S191A

    Depositor

    Mary Mullins

    Article

    Tajer et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

    Insert

    acvr1l (acvr1l Zebrafish)
    

    Use

    Tags

    HA

    Expression

    Mutation

    T186V, S187A, S189A, S191A

    Promoter

    SP6

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge