19,771 results

Showing: 4531 - 4560 of 19771 results

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pET21a-MTF-His

Plasmid

#124133

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Methionyl-tRNA formyltransferase (fmt Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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hCathepsin L

Plasmid

#11250

Purpose

Mammalian expression of human cathepsin L

Depositor

Hyeryun Choe

Article

Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

Has Service

Cloning Grade DNA

Insert

cathepsin L (CTSL Human)

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pET21a(+)-Bst LF-6xHis

Plasmid

#159148

Purpose

expresses His-tagged large fragment of Bst polymerase

Depositor

Andrea Pauli

Article

Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

Insert

large fragment of Geobacillus stearothermophilus DNA polymerase I

Tags

His

Expression

Bacterial

Available Since

Sept. 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Z-lock cofilin

Plasmid

#141137

Purpose

controlling the actin-severing ability of cofilin with light

Depositor

Klaus Hahn

Article

Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

Insert

Zdk2, Cofilin, LOV2

Expression

Mammalian

Mutation

mCherry, Zdk2(132F), Cofilin S3A, LOV WT

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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SacI ML GFP Strand 11 Short

Plasmid

#164121

Purpose

Detect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

Depositor

Marisa Brini, Tito Calì

Article

Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

Insert

SacI ML GFP Strand 11 Short

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCytochrome C-GFP

Plasmid

#41182

Depositor

Douglas Green

Article

Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

Insert

Cytochrome C

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

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Human SERCA2a (pcDNA3.1+)

Plasmid

#75187

Purpose

Mammalian expression of ATP2A2

Depositor

Jonathan Lytton, David MacLennan

Article

Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

Insert

ATP2A2 (ATP2A2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-lyso-pHoenix

Plasmid

#70112

Purpose

Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHoenix (Cd63 Mouse, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat)

Tags

Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pmEGFP_a_tubulin_C1

Plasmid

#21039

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

pmEGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-PARP1-3xFlag-WT

Plasmid

#111575

Purpose

Expresses full length PARP1 in mammalian cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP1 (PARP1 Human)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-HDAC4-FLAG

Plasmid

#30485

Depositor

Tso-Pang Yao

Insert

HDAC4 (HDAC4 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pCAGGS-HA-hUBE1L

Plasmid

#12438

Depositor

Dong-Er Zhang

Article

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

UBE1L (UBA7 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pF4Ag NanoLuc (ATG-42)

Plasmid

#137777

Purpose

Expresses NanoLuc in multi promoter expression vector (CMV andT7 promoters)

Depositor

Lance Encell

Article

Hall et al ACS Chem Biol. 2012 Nov 16;7(11):1848-57

Insert

NanoLuc (Nluc)

Expression

Bacterial and Mammalian

Mutation

NanoLuc contains 16 amino acid changes compared t…

Promoter

T7 and CMV

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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plenti-CAG-gate-FLAG-IRES-GFP

Plasmid

#107398

Purpose

Lentiviral gateway destination vector

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

FLAG

Expression

Mammalian

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pETM6-E12-vioABECD

Plasmid

#66537

Purpose

pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Inserts

VioA
VioB
VioE
VioC
VioD

Use

Synthetic Biology

Expression

Bacterial

Promoter

Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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3XFlagNICD1

Plasmid

#20183

Depositor

Raphael Kopan

Article

Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

Insert

Notch 1 Intracellular Domain (Notch1 Mouse)

Tags

3XFLAG

Expression

Mammalian

Mutation

Contains murine Notch 1 Intracellular Domain (Val…

Promoter

CMV

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV6M-Pak1

Plasmid

#12209

Depositor

Jonathan Chernoff

Article

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET-4xTR-TUBE

Plasmid

#110312

Purpose

E. coli expression and purification of 4xTR-TUBE

Depositor

Yasushi Saeki

Article

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

4xTR-TUBE (UBQLN1 Human)

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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CAG-GFP-IRES-CRE

Plasmid

#48201

Purpose

retroviral vector encoding for GFP and Cre-recombinase

Depositor

Fred Gage

Article

Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

Inserts

eGFP
Cre

Use

Retroviral

Expression

Mammalian

Promoter

CAG and linked by IRES

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-FUS/TLS-FLAGC

Plasmid

#60362

Purpose

Plasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.

Depositor

Sébastien Britton, Patrick Calsou

Article

Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

Insert

FUS (FUS Human)

Tags

FLAG and GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLX-TRV1

Plasmid

#180515

Purpose

Agrobacterium tumefaciens-based expression of TRV1

Depositor

Jose-Antonio Daros

Article

Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:

Insert

Tobacco rattle virus RNA1

Expression

Plant

Promoter

35S CaMV

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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V5-APEX2-LMNA

Plasmid

#129275

Purpose

Expression of APEX2 at nuclear lamina

Depositor

Alice Ting

Article

Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

Insert

V5-APEX2-LMNA

Use

Lentiviral

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEB1deltaC-mCherry

Plasmid

#80603

Purpose

Expresses C-terminally truncated, mCherry-tagged EB1 as marker for growing microtubule plus end

Depositor

Torsten Wittmann

Article

Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. d

Insert

EB1 (MAPRE1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Truncated C-terminus; EB1 coding sequence ends at…

Promoter

CMV

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-HIF1 alpha

Plasmid

#21101

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

HYPOXIA INDUCIBLE FACTOR 1ALPHA (HIF1A Human)

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCAG-rtTA

Plasmid

#163601

Purpose

Expresses rtTA in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

rtTA

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA2-CAND1

Plasmid

#20719

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)

Tags

HA2

Expression

Mammalian

Available Since

April 21, 2009

Availability

Academic Institutions and Nonprofits only
FUW-tetO-MCS

Plasmid

#84008

Purpose

doxycycline-inducible lentiviral vector

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-CMV

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBacPAK8-2xHA-MOF

Plasmid

#29763

Depositor

Joan Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

MYST1 (KAT8 Human)

Tags

2xHA

Expression

Insect

Available Since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only
Proinsulin-NanoLuc in pLX304

Plasmid

#62057

Purpose

Luminescent reporter of insulin secretion

Depositor

David Altshuler

Article

Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

Insert

Proinsulin-NanoLuc (Ins2 Mouse, Synthetic)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

Inserted NanoLuc into C-peptide of mouse Ins2 cod…

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Murine E-cadherin mCherry

Plasmid

#71366

Purpose

mammalian expression of murine E-caderin with mCherry fusion

Depositor

Alpha Yap

Article

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

E-cadherin (Cdh1 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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19,771 results