19,207 results

1. Spectrin-C-cpstFRET

   Plasmid

   #61111

   Purpose

   Expression of Spectrin-C-term cpstFRET to measure mechanical stress and report multidimensional stress fields

   Depositor

   Fred Sachs

   Article

   Meng et al J Cell Sci. 2012 Feb 1;125(Pt 3):743-50.

   Insert

   spectrin-cpstFRET
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. CARPID BASU-dCasRx

   Plasmid

   #153209

   Purpose

   express CARPID BASU-dCasRx fusion protein in mammalian cells

   Depositor

   Liang Zhang, Jian Yan

   Article

   Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

   Insert

   BASU-dCasRx
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   R239A/H244A/R858A/H863A in RfxCas13d

   Promoter

   EF-1a

   Available since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5M-ADAM10

   Plasmid

   #31717

   Depositor

   Rik Derynck

   Article

   Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

   Insert

   ADAM10 (ADAM10 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCherry.90 beta

   Plasmid

   #108223

   Purpose

   Mammalian expression vector for mCherry fusion to human Hsp90 beta

   Depositor

   Didier Picard

   Article

   Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

   Insert

   mCherry-Hsp90b (HSP90AB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.Syn.GCaMP6s.WPRE.SV40

   Plasmid

   #100843

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

   Promoter

   Synapsin

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLSB-NAT

   Plasmid

   #166698

   Purpose

   Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.

   Depositor

   Robin Allshire

   Article

   Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

   Inserts

   tRNA promoter : sgRNA cassette
   adh15 promoter : Cas9 codon-optimised for S. pombe
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   adh15 promoter and tRNA promoter

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Human_TFAM_NoMTS_pET28

   Plasmid

   #34705

   Depositor

   David Chan

   Article

   Ngo et al Nat Struct Mol Biol. 2011 Oct 30;18(11):

   Insert

   TFAM (TFAM Human)
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Encodes residues 43–246, corresponding to full-le…

   Promoter

   T7

   Available since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV2-hSyn-G-Flamp1

   Plasmid

   #188569

   Purpose

   green biosensor for cAMP in neurons

   Depositor

   Jun Chu

   Article

   Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

   Insert

   G-Flamp1
   

   Use

   AAV

   Tags

   His tag

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. At2S3:RUBY

   Plasmid

   #160906

   Purpose

   RUBY under the control of At2S3 promoter/marker for Arabidopsis transgenes

   Depositor

   Yunde Zhao

   Article

   He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

   Insert

   RUBY and At2S3 promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   At2S3

   Available since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TfR-pHuji

    Plasmid

    #61505

    Purpose

    Human transferrin receptor tagged with pH sensitive red fluorescent protein pHuji

    Depositor

    David Perrais

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    Transferrin receptor (TFRC Human)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJKR-H-tetR

    Plasmid

    #62561

    Purpose

    Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG32

    Plasmid

    #35122

    Depositor

    John McCusker

    Article

    Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

    Insert

    hphMX4
    

    Use

    Yeast replicating

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-N1 hMOG-alpha1-EGFP

    Plasmid

    #126463

    Purpose

    Expression a human MOG (isoform alpha1) EGFP fusion protein in mammalian cells

    Depositor

    Markus Reindl

    Article

    Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

    Insert

    Myelin oligodendrocyte glycoprotein (MOG Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLv-HSA-uDys/eGFP

    Plasmid

    #26810

    Depositor

    Jeffrey Chamberlain

    Article

    Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

    Insert

    dystrophin (DMD Human)
    

    Use

    Lentiviral; Lentiviral plasmid backbone from l. n…

    Tags

    eGFP

    Expression

    Mutation

    deleted R4-R23 deleted exons 71-78

    Promoter

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMVTNT PINK1 C-myc

    Plasmid

    #13314

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-HA-UTX

    Plasmid

    #24168

    Purpose

    Mammalian expression of human UTX with HA tag

    Depositor

    Kristian Helin

    Article

    Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

    Insert

    UTX (KDM6A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE30-His-CKM

    Plasmid

    #124134

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Creatine kinase (CKM Chicken)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDIRECT_23C

    Plasmid

    #91140

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. 3X FLAG-FUS-WT

    Plasmid

    #44985

    Depositor

    Robin Reed

    Article

    Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

    Insert

    FUS (Full length) (FUS Human)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV Puro

    Plasmid

    #68469

    Purpose

    Retroviral plasmid with multiple cloning site and puromycin resistance

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Puromycin Resistance
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3.1-Peredox-mCherry-NLS

    Plasmid

    #32384

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    Peredox-mCherry-NLS
    

    Use

    Tags

    Nuclear Localization Seq

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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22. pTO_CHMP4B_LAP

    Plasmid

    #101850

    Purpose

    Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

    Depositor

    Daniel Gerlich

    Article

    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

    Insert

    Charged Multivesicular Body Protein 4B (CHMP4B Human)
    

    Use

    Gateway cloning, flp-frt recombination, tet-induc…

    Tags

    Localization and Affinity Purification (LAP) tag

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. EF12J

    Plasmid

    #42943

    Depositor

    Eline Luning Prak

    Article

    Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

    Insert

    EN+ Human L1 element (L1RE1 Human)
    

    Use

    Tags

    EGFP (opposite strand)

    Expression

    Mammalian

    Mutation

    AgeI-BclI fragment swapped with nt 1927-3708 from…

    Promoter

    CMV MIE, L1 5'UTR

    Available since

    April 25, 2013

    Availability

    Academic Institutions and Nonprofits only

24. pSFV-Helper1

    Plasmid

    #92073

    Purpose

    This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.

    Depositor

    Henrik Garoff

    Article

    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

    Insert

    cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
    

    Use

    Helper plasmid, for run-off transcription and rna…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTRE-tdTomato

    Plasmid

    #50798

    Purpose

    Expression of tdTomato under the control of the TRE-promoter

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    TREtight driven tdTomato fluorescent protein
    

    Use

    Mutli-species

    Tags

    Expression

    Mutation

    Promoter

    TREtight

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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26. PT2/C-Luc//PGK-SB13

    Plasmid

    #20207

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    Flirefly luciferase/Sb transposase
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHN924

    Plasmid

    #178885

    Purpose

    Expression of GST-His8-HRV3C

    Depositor

    Kazuhiro Abe

    Insert

    human rhinovirus 3C protease
    

    Use

    Tags

    GST, His8

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBbA5c-MevT(CO)-MBIS(CO, ispA)

    Plasmid

    #35151

    Depositor

    Taek Soon Lee, Jay Keasling

    Article

    Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

    Insert

    AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    R364S in Mevalonate Kinase

    Promoter

    LacUV5,

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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29. pMRX-No-HaloTag7-mGFP-LC3

    Plasmid

    #184901

    Purpose

    Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Use

    Tags

    HaloTag7 and monomeric EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSC101-Donor

    Plasmid

    #140630

    Purpose

    Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    LE, RE, KanR, temperature sensitive pSC101 origin of replication
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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