We narrowed to 12,703 results for: NUC

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HA-GFP1-10-pDEST-C

Plasmid

#118369

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-GFP1-10

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pDIV643

Plasmid

#226182

Purpose

Expresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

uidA marker flanked with targeting regions to KU70 gene in K.phaffii

Expression

Bacterial

Promoter

TEF1p

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1553 - pAAV EF1a EGFP-KASH

Plasmid

#131682

Purpose

An adeno-associated viral vector expressing nuclear envelope-embedded eGFP

Depositor

Christopher Richie

Article

Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

Insert

EGFP-KASH

Use

AAV

Tags

KASH

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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p15a_AcCas12n

Plasmid

#203806

Purpose

p15a-based plasmid containing a rpsL driven AcCas12n cassette and the araBAD-driven lambda-red recombinase cassette. Apramycin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12n

Use

CRISPR

Promoter

rpsl

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSGKP_AcsgRNA

Plasmid

#203807

Purpose

pSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcgRNA

Use

CRISPR

Promoter

J23119

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pICH86966::AtU6p::sgRNA_PDS

Plasmid

#46966

Purpose

Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

Depositor

Sophien Kamoun

Article

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p::sgRNA_PDS

Use

CRISPR; Plant expression

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-p53

Plasmid

#12091

Depositor

Tyler Jacks

Article

Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

Insert

p53 (TP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

72R variant of p53

Available Since

July 5, 2006

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(TCA3)

Plasmid

#235064

Purpose

Expresses Tad-CBE(TCA3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(TCA3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-chiRNA

Plasmid

#45946

Purpose

Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Article

Gratz et al Genetics. 2013 May 24.

Insert

U6-BbsI-chiRNA

Use

CRISPR

Expression

Insect

Promoter

Dm-snRNA:U6:96Ab

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only

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pSQT1601

Plasmid

#53369

Purpose

human codon optimized Csy4 and FokI-dCas9 expression plasmid

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

hCsy4-T2A-NLS-hFokI-dCas9-NLS

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

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pFYF1320 EGFP Site#1

Plasmid

#47511

Purpose

human gRNA expression vector targeting EGFP

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EGFP site 1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pdCas9

Plasmid

#46569

Purpose

Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

Depositor

Luciano Marraffini

Article

Bikard et al Nucleic Acids Res. 2013 Jun 12.

Inserts

tracrRNA
dcas9
CRISPR array

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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T2A 3xNLS GFP

Plasmid

#227721

Purpose

Plasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp Resistant

Depositor

Oliver Hobert, Haosheng Sun

Article

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

T2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)

Use

Cloning vector

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-NPM WT

Plasmid

#17578

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

EGFP and Flag

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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p414-TEF1p-Cas9-CYC1t

Plasmid

#43802

Purpose

A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

Depositor

George Church

Article

Dicarlo et al Nucleic Acids Res

Insert

Human Optimized S. pyogenes Cas9

Use

CRISPR

Expression

Yeast

Promoter

TEF1 promoter

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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pLSU4cg10

Plasmid

#126078

Purpose

Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

longCaMV35S

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-Cas9-T2A-GFP

Plasmid

#53190

Purpose

3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pTEI165

Plasmid

#126074

Purpose

Vector for expression of plastid targeted improved YFP1-10 (T65G,T205S, T203Y) in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

YFP1-10 (improved)

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI-CMV-GFP-TREX1 BLAST

Plasmid

#164228

Purpose

lentiviral plasmid for GFP-hTREX1

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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PB-CAG-GFPd2

Plasmid

#115665

Purpose

Piggybac transposon plasmid for destabilized eGFP (GFPd2)

Depositor

Jordan Green

Article

Rui et al ACS Appl Mater Interfaces. 2019 Feb 22.

Insert

GFPd2

Use

Synthetic Biology

Tags

Destabilization

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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T2A GFP H2B

Plasmid

#227722

Purpose

Plasmid contains T2A GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

Depositor

Oliver Hobert, Haosheng Sun

Article

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

(nlp-51 homology) T2A 3xNLS GFP (nlp-51 Nematode)

Use

Cloning vector

Tags

T2A 3xNLS GFP

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pSB1C3-pT7-sfGFP_RED20

Plasmid

#135173

Purpose

Expresses sfGFP recoded in the RED20 genetic code off the T7 promoter

Depositor

Drew Endy

Article

Calles et al Nucleic Acids Res. 2019 Sep 12. pii: 556

Insert

sfGFP_RED20

Use

Synthetic Biology

Mutation

Recoded ORF of sfGFP to only use one codon per am…

Promoter

taatacgactcactata

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pX330S-2

Plasmid

#58778

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL90003

Plasmid

#68262

Purpose

Level 0 Golden Gate Part: Promoter U6 (Triticum aestivum)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter: U6 (Triticum aestivum)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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YFP-RPS5-C1

Plasmid

#134534

Purpose

Expresses YFP-RPS5 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

RPS5 (RPS5 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDae079 (eMutaT7[transition])

Plasmid

#187622

Purpose

Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Inserts

TadA-8e-T7RNAP
PmCDA1-T7RNAP

Expression

Bacterial

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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YFP-RPL23a-C1

Plasmid

#134535

Purpose

Expresses YFP-RPL23a fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

RPL23a (RPL23A Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET-Expotin 1

Plasmid

#42047

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

Exportin-1 (XPO1 Human)

Tags

His

Expression

Bacterial

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only

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MS2-epegRNA-Dnmt1

Plasmid

#211372

Purpose

Dnmt1 targeting epegRNA with MS2 insertion in gRNA scaffold for v3 PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MS2-epegRNA

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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double cut NH-donor

Plasmid

#83576

Purpose

eGFP reporter donor containing double SA cutting sites for NHEJ knock-in

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

eGFP

Use

Nhej donor

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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