We narrowed to 9,659 results for: SUB;
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Plasmid#161942PurposeCarries Golden Gate substitution insert for acVHH-assisted bisection mappingDepositorInsertpSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
ExpressionBacterialPromoterPBADAvailable SinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Banshee-ShCit-A
Plasmid#85216PurposeShort hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.DepositorInsertshCit-A
UseRetroviralExpressionMammalianPromoterH1Available SinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Banshee-ShCit-B
Plasmid#85217PurposeShort hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.DepositorInsertshCit-B
UseRetroviralExpressionMammalianPromoterH1Available SinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_Mito
Plasmid#65671PurposeExpresses Mito-ONL in mammalian cellsDepositorInsertcytochrome c oxidase subunit VIII
UseLuciferaseTagsONLExpressionMammalianPromoterCMVAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-OR-MalT-3NT
Plasmid#62309Purposesynthetic circuit: OR gate, the sgRNA used for the NOT gate was substituted with one designed to target the malT gene in the E. coli genomeDepositorInsertsA2NT sgRNA
Malt-3NT sgRNA
A2NT sgRNA
UseCRISPRExpressionBacterialPromoterPA2, PPhlF, and pBADAvailable SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCeSR beta deltaN
Plasmid#13728DepositorInsertSignal recognition particle receptor β subunit (R186.3 Nematode)
Tags6xHisExpressionBacterialMutationexpresses residues 38-240 of C. elegans SRβ with …Available SinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pScSR beta deltaN Flag
Plasmid#13729DepositorInsertSignal recognition particle receptor β subunit (SRP102 Budding Yeast)
Tags6xHis and FlagExpressionBacterialMutationexpresses residues 31-244 of S. cerevisiae SRβ wi…Available SinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSF3-ultraID
Plasmid#172878Purposeexpresses ultraID in mammalian cells with the tet-on or tet-off systemDepositorInsertultraID
UseLuciferase and Retroviral; To be used in a tet-on…TagsmycExpressionMammalianMutationfragment ([aa2-171]) of aquifex aeolicus BirA wit…PromoterTRE-cmvAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cyto-ExRai-AktAR2
Plasmid#184048PurposeCytosol-targeted ExRai-AktAR2.DepositorInsertCyto-ExRai-AktAR2
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMitoTimer
Plasmid#52659PurposeExpresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivoDepositorInsertDsRed1-E5
UseDsredTagshuman cytochrome c oxidase subunit VIII mitochond…PromoterCMVAvailable SinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSF3-microID
Plasmid#172880Purposeexpresses microID in mammalian cells with the tet-on or tet-off systemDepositorInsertmicroID
UseLuciferase and Retroviral; To be used in a tet-on…TagsmycExpressionMammalianMutationfragment ([aa2-171]) of aquifex aeolicus BirA wit…PromoterTRE-cmvAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-endo-Nb37
Plasmid#205514PurposeEndosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.DepositorInsertendo-Nb37
Tags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…ExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Luciferase-IRES-NrasG12D
Plasmid#60834PurposeMouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRESDepositorInsertNrasG12D (Nras Mouse)
UseRetroviralAvailable SinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-Rac1
Plasmid#83950PurposeRac1 FLIM donor for dynamic imaging of GTPase activityDepositorAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-BaLgp120-8his
Plasmid#100925PurposeMammalian expression plasmid for gp120 subunit from the BaL HIV-1 strainDepositorInsertHIV-1 (BaL) Env
TagsCD5 leader sequence and Purification tag after K5…ExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-mCardinal (JDW 1418)
Plasmid#242553PurposeGateway middle entry clone containing H2B-mCardinal; Nuclear far red fluorescent reporterDepositorInsertmCardinal
UseGateway subcloningAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-AktAR2
Plasmid#184047PurposeImproved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.DepositorInsertExRai-AktAR2
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianPromoterCMVAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant SigmaR1-mNeonGreen R119A F191A
Plasmid#226574PurposeEncodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
TagsmNeonGreenExpressionMammalianMutationR119A, F191AAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
CaV2.1 (pcDNA6)
Plasmid#26578DepositorAvailable SinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
p-eGFP-beta 2 CP
Plasmid#13298DepositorAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMXSIP-FLAG-ATPIF1
Plasmid#204703Purposeexpresses FLAG-ATPIF1 in mammalian cellsDepositorAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO mTRPM7
Plasmid#45482DepositorInsertTRPM7 (Trpm7 Mouse)
UseTetracycline inducibleTagsFlagExpressionMammalianPromoterTetracycline-controlled CMVAvailable SinceMay 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
CoxVIIIx2-OeNL(Ca2+)_18μ-pcDNA3
Plasmid#111924PurposeOrange color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of OeNL(Ca2+)_18μDepositorInsertOeNL(Ca2+)_18μ
TagsCoxVIIIx2ExpressionMammalianMutationE67D, E104D, D133E at CaMPromoterCMVAvailable SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3
Plasmid#111923PurposeCyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19μDepositorInsertCeNL(Ca2+)_19μ
TagsCoxVIIIx2ExpressionMammalianMutationE67D, F92W, E104D, D133E at CaMPromoterCMVAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)
Plasmid#70191PurposeDerived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.DepositorInsertClpX (clpX )
Available SinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
SP6-VEE-GFP
Plasmid#58976Purposeexpression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA repliconDepositorInsertEGFP
UseVenezuelan equine encephalitis (vee) virus rna re…ExpressionMammalianPromoter26S subgenomic promoterAvailable SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRA-30-picky_sticky
Plasmid#138983PurposeIVT template for the alpha subunit of the mouse TCR #30 that is reactive against MC38DepositorAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRB-33-MC_hammer
Plasmid#138990PurposeIVT template for the beta subunit of the mouse TCR #33 that is reactive against MC38DepositorAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRB-32-riot_punisher
Plasmid#138988PurposeIVT template for the beta subunit of the mouse TCR #32 that is reactive against MC38DepositorAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRA-32-riot_punisher
Plasmid#138987PurposeIVT template for the alpha subunit of the mouse TCR #32 that is reactive against MC38DepositorAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRB-31-murderous_crow
Plasmid#138986PurposeIVT template for the beta subunit of the mouse TCR #31 that is reactive against MC38DepositorAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRA-31-murderous_crow
Plasmid#138985PurposeIVT template for the alpha subunit of the mouse TCR #31 that is reactive against MC38DepositorAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRB-30-picky_sticky
Plasmid#138984PurposeIVT template for the beta subunit of the mouse TCR #30 that is reactive against MC38DepositorAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ss-SigmaR1DTM-mNeonGreen R119A F191A
Plasmid#226576PurposeEncodes mutant siRNA resistant SigmaR1 protein (transmembrane region deletion + substitutions) labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
TagsmNeonGreenExpressionMammalianMutationR119A, F191AAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lyn FAK biosensor
Plasmid#78299PurposeFRET biosensor. Visualization of FAK activity at lipid rafts in live cellsDepositorInsertLyn Fak biosensor
ExpressionMammalianPromoterCMVAvailable SinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-STOPS
Plasmid#175006PurposeAkt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity.DepositorInsertmCherry-Akt-STOPS
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-DsRed2
Plasmid#112219PurposeCloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2DepositorInsertDsRed2
UseCRISPRExpressionMammalianMutationDsRed2 contains a silent nucleotide substitution …Available SinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PB1-3x-FLAG-B/BRISBANE/60/2008
Plasmid#187918PurposeExpresses C terminal 3xFLAG tagged PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cellsDepositorInsertpolymerase basic protein 1
Tags3xFLAGPromoterCMV PromoterAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nuc-ExRai-AktAR2
Plasmid#184049PurposeNuclear-targeted ExRai-AktAR2.DepositorInsertNuc-ExRai-AktAR2
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_iSH2-pMag(3x)-iRFP
Plasmid#67304PurposeBlue light dependent photoswitching systemDepositorAvailable SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
ePop
Plasmid#50953PurposeExpresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillationsDepositorInsertsE lysis protein
LuxI
LuxR
MutationEarly stop codon introduced in mutated luxR resul…Available SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PB2-3x-FLAG-B/BRISBANE/60/2008
Plasmid#187916PurposeExpresses C terminal 3xFLAG tagged PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cellsDepositorInsertpolymerase basic protein 2
Tags3xFLAGExpressionMammalianPromoterCMV PromoterAvailable SinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pET15b CnA CnB
Plasmid#11787DepositorAvailable SinceMay 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
Sc++
Plasmid#155011PurposeExpresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cellsDepositorInsertSc++
ExpressionMammalianMutationT1227K, Loop Substitution from S. anginosusPromoterEF1ɑAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE7.10-P2A-GFP
Plasmid#162509PurposeExpress ABE7.10-P2A-GFP by CMV promoter in mammalian cellsDepositorInsertABE7.10-P2A-GFP
UseCRISPRTagsNLSExpressionMammalianPromoterCMVAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-ExRai-AktAR2
Plasmid#184052PurposeLysosome-targeted ExRai-AktAR2.DepositorInsertLyso-ExRai-AktAR2
TagsLysosome-associated membrane protein 1 (LAMP1).ExpressionMammalianPromoterCMVAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_iSH2-pMagFast2(3x)-iRFP
Plasmid#67298PurposeBlue light dependent photoswitching systemDepositorAvailable SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXJ40 GADD34 (Delta Pest1)
Plasmid#78096Purposemammalian expression of GADD34 Delta Pest1DepositorInsertGADD34 (PPP1R15A Human)
TagsFLAGExpressionMammalianMutationdeletion of PEST1PromoterCMVAvailable SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXJ40 GADD34 (514-674aa)
Plasmid#75480Purposemammalian expression of GADD34 514-674 aaDepositorInsertGADD34 (PPP1R15A Human)
TagsFLAGExpressionMammalianMutationexpresses aa 514-674PromoterCMVAvailable SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only