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We narrowed to 14,223 results for: Cas9

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Showing: 3061 - 3080 of 14223 results

  3. pLenti_EF1α-NES-Kinprola_PKA_T/A-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid
    #233372
    Purpose
    EF1α driven co-expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A fused to mEGFP and Cas9 expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Publication
    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038
    Inserts
    NES-Kinprola_PKA_T/A-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    Use
    Lentiviral
    Tags
    FLAG, NES, and mEGFP
    Promoter
    EF1α
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233372-plasmid-map-sequence-id-466777

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  5. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104911
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    104911-plasmid-map-sequence-id-269032

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  10. pDual_dsCas9_Puro_hs_NC_chr1

    Plasmid
    #214688
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Mutation
    Puromycin resistance cassette has silent mutation…
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214688-plasmid-map-sequence-id-428817

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  11. pCMV-eNme2-T.1-ABE8e

    Plasmid
    #185668
    Purpose
    Expresses eNme2-T.1-ABE8e in mammalian cells
    Depositor
    David Liu
    Publication
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ecTadA(8e)-neNme2-T.2
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…
    Available since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    185668-plasmid-map-sequence-id-373131

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  12. FLAG-LoxP-PURO-LoxP-SNAP-TERT

    Plasmid
    #71390
    Purpose
    Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.
    Depositor
    Thomas Cech
    Publication
    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:
    Insert
    TERT (TERT Human)
    Use
    Hr donor
    Tags
    FLAG-SNAP
    Promoter
    Endogenous TERT promotor
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71390-plasmid-map-sequence-id-128451

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  13. pORANGE GFP-Camk2a KI

    Plasmid
    #131484
    Purpose
    Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)
    Depositor
    Harold MacGillavry
    Publication
    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA and GFP donor (Camk2a, Camk2a Rat, Mouse)
    Expression
    Mammalian
    Promoter
    U6 and Cbh
    Available since
    Sept. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131484-plasmid-map-sequence-id-257183

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  14. pCAG-spCas9-N57

    Plasmid
    #169920
    Purpose
    Expression of SpCas9-N57 fusion protein to enhace site-specific DNA integration.
    Depositor
    Zhili Rong
    Publication
    Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-N57
    Use
    CRISPR
    Mutation
    N57 is the PAI domain of SB100X
    Promoter
    CAG
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    169920-plasmid-map-sequence-id-336052

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  16. pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

    Plasmid
    #187963
    Purpose
    mAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
    osTIR1
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker and T2A
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187963-plasmid-map-sequence-id-373709

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  18. pLoxP-DHFR-mCherry

    Plasmid
    #70147
    Purpose
    Expresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletion
    Depositor
    David Sibley
    Publication
    Long et al Infect Immun. 2016 Jan 11. doi: 10.1128/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    a selectable dihydrofolate reductase-thymidylate synthase marker
    Use
    Expression in toxoplasma gondii
    Tags
    mCherry
    Promoter
    DHFR 5' UTR
    Available since
    Nov. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70147-plasmid-map-sequence-id-423191

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Showing: 3061 - 3080 of 14223 results