We narrowed to 5,635 results for: pcas

1. pBS-U6-crRNA-empty

   Plasmid

   #134921

   Purpose

   Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   crRNA for CRISPR-Cas3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CP1028-ABE8e

   Plasmid

   #138492

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSpCas9 (CP1028)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CP1041-ABE8e

   Plasmid

   #138493

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSpCas9 (CP1041)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TC1332

   Plasmid

   #164874

   Purpose

   CURE-N: C to U RNA Editing, with high efficiency and low off-target effects

   Depositor

   Tian Chi

   Article

   Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

   Insert

   hAPOBEC3A fused to PspCas13b (APOBEC3A )
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   human apobec Y132D

   Promoter

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMTKM05

   Plasmid

   #233477

   Purpose

   SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and Hyg selection.

   Depositor

   Vincent Martin

   Article

   Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SPDK3860

   Plasmid

   #199245

   Purpose

   Modified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) gene

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
   

   Use

   T-dna vector

   Tags

   Expression

   Mutation

   Promoter

   pPEBV

   Available since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LLP791_PB_dCas9-SSSavi_BirA

   Plasmid

   #211786

   Purpose

   PiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   dCas9, Spy, Snoop, aGCN4, AviTags
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   dCas9 D10A and H840A

   Promoter

   pCAGG abd pEF1a

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CAG-SaCas9-WPRE

   Plasmid

   #89996

   Purpose

   Expresses high levels of WT SaCas9 for genome editing

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

   Insert

   SaCas9 (NEWENTRY Staphylococcus aureus)
   

   Use

   Tags

   3xHA, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. dCas9-10xSunTag(22aa)

   Plasmid

   #215745

   Purpose

   Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   dCas9-10xSunTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken beta-actin promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX330-PITCh-H2BC11

    Plasmid

    #207755

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBSE901

    Plasmid

    #91709

    Purpose

    CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV-U6-gRNA scaffold-UbC-mCherry-PuroR

    Plasmid

    #232183

    Purpose

    Lentiviral plasmid for cloning an spCas9 gRNA with puromycin resistance and mCherry

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, hUbC

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUDP004

    Plasmid

    #101165

    Purpose

    E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YE2-BE4max

    Plasmid

    #138156

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE2)-nSpCas9-UGI-UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R132E; within Cas9 D10A

    Promoter

    CMV

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. BPK1520_puroR

    Plasmid

    #173901

    Purpose

    SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection

    Depositor

    Ronald Cohn

    Article

    Erwood et al Genome Res. 2019 Dec;29(12):2010-2019. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/U6

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pH-mono-MAD7

    Plasmid

    #170134

    Purpose

    For plant prime editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al J Genet Genomics. 2021 May 3. pii: S1673

    Insert

    MAD7(ErCas12a)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    maize Ubiquitin-1, switchgrass Ubiquitin-1

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB-Aka-P2A-GFP

    Plasmid

    #120867

    Purpose

    Piggybac transposon plasmid for live animal optical imaging

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    Akaluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    insert Akaluc and a P2A site

    Promoter

    CAG Promoter

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ePPEmax*

    Plasmid

    #205244

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Detailed in manuscript

    Promoter

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Double UP mNeon to mScarlet Rac1-V12

    Plasmid

    #125136

    Purpose

    Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-V12 (Constitutively Active).

    Depositor

    Erik Dent

    Article

    Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

    Insert

    mNeon/mScarletP2A Rac1-V12
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Removed Not1 site in mScarlet. 12th AA in Rac1 ch…

    Promoter

    CAG

    Available since

    May 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBCW

    Plasmid

    #62658

    Purpose

    Mammalian expression of PhiC31o recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    PhiC31
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHL-FcHis

    Plasmid

    #99846

    Purpose

    mammalian expression with secretive signal and FC tag

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EPHB2 Human)
    

    Use

    Tags

    Fc_His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TC1681

    Plasmid

    #164872

    Purpose

    CURE-C2: C to U RNA Editing, with high efficiency and high off-target effects

    Depositor

    Tian Chi

    Article

    Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

    Insert

    hAPOBEC3A fused to PspCas13b (APOBEC3A )
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    human apobec Y132D

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TDP43 NOTAG1

    Plasmid

    #28206

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Full length

    Promoter

    Available since

    July 1, 2011

    Availability

    Academic Institutions and Nonprofits only

24. pORANGE LOX Cloning template vector

    Plasmid

    #139651

    Purpose

    Cloning template for ORANGE-CAKE conditional knock-in constructs

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p2T-CMV-eA3A-BE5-NG-BlastR

    Plasmid

    #152998

    Purpose

    High purity C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GB4542

    Plasmid

    #215261

    Purpose

    Module for the expression of the N. nambi HISPS gene under the regulation of the copper inducible CBS4x:mDFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    CBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GB4401

    Plasmid

    #215252

    Purpose

    Module for the expression of the N. nambi HISPS gene under the regulation of PNOS and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    SF-PNOS:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pX330-PITCh-PXN

    Plasmid

    #227315

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PXN for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of PXN (PXN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pX458-ECFP

    Plasmid

    #112220

    Purpose

    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

    Depositor

    Yinming Liang

    Article

    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

    Insert

    ECFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. NG-ABE8-dimer

    Plasmid

    #138497

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSpCas9 (NG)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge