We narrowed to 7,181 results for: RAP

1. pADH119

   Plasmid

   #90985

   Purpose

   NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   gRNA conserved, C. albicans LEU2 2 of 2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pH6HTC_HNRNPC-Halo

   Plasmid

   #175306

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   HNRNPC (HNRNPC Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1

   Plasmid

   #81133

   Purpose

   mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Inserts

   Zdk1
   VAV2 DH/PH/C1 (VAV2 Human)
   

   Use

   Tags

   mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR221-CAG.Pro

   Plasmid

   #79490

   Purpose

   MultiSite Gateway entry clones, first fragment, (attL1, attR5) CAG promoter

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   CAG Promoter
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   CAG Promoter

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-MinC

   Plasmid

   #133628

   Purpose

   Expression vector for the Escherichia coli MinC with an N-terminal fusion of a His-tag for purification.

   Depositor

   Petra Schwille

   Article

   Loose et al Nat Struct Mol Biol. 2011 May;18(5):577-

   Insert

   His-MinC (minC E. coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. SBP-EGFP-Ecadherin_puromycin

   Plasmid

   #65292

   Purpose

   Ecadherin reporter only (no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Ecadherin
   

   Use

   Tags

   EGFP and IL-2 Signal Sequence and Streptavidin Bi…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOZ-N-FH BRCC36

   Plasmid

   #27496

   Depositor

   Roger Greenberg

   Article

   Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

   Insert

   BRCC36 (BRCC3 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pTRE:CloverCP-EFS:ZeoR

   Plasmid

   #68478

   Purpose

   Lentivirus for establishment of doxycycline-inducible cell line expression CloverCP

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   CloverCP
   Zeocin Resistance
   

   Use

   Lentiviral

   Tags

   "CP" degradation domain

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and pTRE

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAdv/TERT

   Plasmid

   #26744

   Depositor

   Bingliang Fang

   Article

   Gu et al Cancer Res. 2000 Oct 1. 60(19):5359-64.

   Insert

   human TERT promoter (TERT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETx-SR6c3

    Plasmid

    #176211

    Purpose

    Bacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein

    Depositor

    Aviv Regev

    Article

    Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

    Insert

    SR6c3
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. p119 (FUGW-UbCp-rtTA3)

    Plasmid

    #105171

    Purpose

    ubiquitin C promoter drives rtTA3 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    UbCp-rtTA3
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBx1-VHH template1-3XMyc-Spacer

    Plasmid

    #176208

    Purpose

    PCR template for generating CeVICA VHH/nanobody library

    Depositor

    Aviv Regev

    Article

    Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

    Insert

    VHH template1-3XMyc-Spacer
    

    Use

    Tags

    Myc tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Expression scaffold for dCas9+sgRNA

    Plasmid

    #171799

    Purpose

    PICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNA

    Depositor

    Stephen Elledge

    Article

    Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

    Insert

    PICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. 4XBS2WT-Luc

    Plasmid

    #16593

    Depositor

    Bert Vogelstein

    Article

    Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

    Insert

    p53 binding sites (TP53 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pVCERC-5 Pvan-dcas9 (Sth3)

    Plasmid

    #133319

    Purpose

    expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    dcas9 (Streptococcus thermophilus #3)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    vanillate

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRMTK-clo6

    Plasmid

    #203957

    Purpose

    Cloning plasmid containing the kanamycin resistance gene insert

    Depositor

    Nathan Crook

    Article

    van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

    Insert

    Kanamycin Resistance Gene
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pH6HTC_mmHUR-Halo

    Plasmid

    #175311

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    HUR (Elavl1 Mouse)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21(+)-His6-Ubc13

    Plasmid

    #212715

    Purpose

    Bacterial expression of His6-Ubc13

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    Ubc13
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pH6HTC_CCDC59-Halo

    Plasmid

    #175327

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    CCDC59 (CCDC59 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. MTK8b_004

    Plasmid

    #123892

    Purpose

    Encodes the 3rd gen. 5' HIV LTR cassette as a type 8b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    5' HIV LTR (3rd gen.)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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