We narrowed to 27,292 results for: Tat

1. pRRLHygro-pEF1a-p53ashL344P-mKate2-splitmVenusC

   Plasmid

   #69587

   Purpose

   Expresses mutated p53-L344P tagged with mKate2 and split C-term mVenus

   Depositor

   Galit Lahav

   Article

   Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

   Insert

   p53-L344P (TP53 Human)
   

   Use

   Lentiviral

   Tags

   mKate2 and split mVenus - C term

   Expression

   Mammalian

   Mutation

   p53 L344P mutation that prevents dimerization and…

   Promoter

   EF1alpha

   Available since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA4 h-Stx3 resistant to shRNA #304-2xMyc/His

   Plasmid

   #99746

   Purpose

   Expresses human Stx3 with a C-term myc-myc-his that is resistant to shRNA #304 from Sigma Aldrich.

   Depositor

   Thomas Weimbs

   Article

   Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

   Insert

   Stx3 (STX3 Human)
   

   Use

   Tags

   myc-myc-his

   Expression

   Mammalian

   Mutation

   Made six (6) silent mutations to confer resistanc…

   Promoter

   CMV/TO

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4-His-Max-A-PQBP1-Y65C

   Plasmid

   #25803

   Depositor

   Marius Sudol

   Article

   Tapia et al J Biol Chem. 2010 Jun 18;285(25):19391-4

   Insert

   PQBP1 with Y65C mutation (PQBP1 Human)
   

   Use

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   Tyrosine 65 within the WW domain of PQBP1 is chan…

   Promoter

   Available since

   July 21, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pChuk

   Plasmid

   #87033

   Purpose

   express IKKalpha in mammalian cells

   Depositor

   Georgios Stathopoulos

   Article

   Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

   Insert

   IKKalpha (Chuk Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pαH-S-GSAS-deltaH69/V70

   Plasmid

   #171744

   Purpose

   Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-deltaH69/V70 variant

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Science. 2021 Jun 24. pii: science.abi62

   Insert

   Spike (S-GSAS-D614G-deltaH69/V70 variant) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIS1 mPELO

   Plasmid

   #60805

   Purpose

   Luciferase reporter to measure miR-155 mediated differential repression. Contains PELO 3' UTR and mutated miR-155 sites

   Depositor

   David Bartel

   Article

   Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

   Insert

   PELO 3'UTR and mutated miR-155 binding site (PELO Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   mutated miR-155 binding site

   Promoter

   thymidine kinase (HSV-TK promoter)

   Available since

   Oct. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIS1 mATP2B1

   Plasmid

   #60789

   Purpose

   Luciferase reporter to measure miR-155 mediated differential repression. Contains ATP2B1 3' UTR and mutated miR-155 sites

   Depositor

   David Bartel

   Article

   Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

   Insert

   ATP2B1 3'UTR and mutated miR-155 binding site (ATP2B1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   mutated miR-155 binding site

   Promoter

   thymidine kinase (HSV-TK promoter)

   Available since

   Oct. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pαH-S-GSAS-D614G-N501Y

   Plasmid

   #171748

   Purpose

   Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-N501Y variant

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Science. 2021 Jun 24. pii: science.abi62

   Insert

   Spike (S-GSAS-D614G-N501Y variant) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pαH-S-GSAS-D614G-I692V

   Plasmid

   #171746

   Purpose

   Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-I692V variant

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Science. 2021 Jun 24. pii: science.abi62

   Insert

   Spike (S-GSAS-D614G-I692V variant) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   July 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pαH-S-GSAS-D614G-Y453F

    Plasmid

    #171745

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-Y453F variant

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-D614G-Y453F variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIS1 mINSIG2

    Plasmid

    #60799

    Purpose

    Luciferase reporter to measure miR-155 mediated differential repression. Contains INSIG2 3' UTR and mutated miR-155 sites

    Depositor

    David Bartel

    Article

    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

    Insert

    INSIG2 3'UTR and mutated miR-155 binding site (INSIG2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    mutated miR-155 binding site

    Promoter

    thymidine kinase (HSV-TK promoter)

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIS1 mELOVL6

    Plasmid

    #60791

    Purpose

    Luciferase reporter to measure miR-155 mediated differential repression. Contains ELOVL6 3' UTR and mutated miR-155 sites

    Depositor

    David Bartel

    Article

    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

    Insert

    ELOVL6 3'UTR and mutated miR-155 binding site (ELOVL6 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    mutated miR-155 binding site

    Promoter

    thymidine kinase (HSV-TK promoter)

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tom20-mChF-AIP(TD)

    Plasmid

    #61521

    Purpose

    Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TD mutation

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    FK506 binding protein 1A (FKBP1A Human)
    

    Use

    Tags

    AIP with TD mutation (see comments), Tom20, and m…

    Expression

    Mammalian

    Mutation

    TD mutation (see comments)

    Promoter

    CMV

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pIS1 mLMBRD2

    Plasmid

    #60801

    Purpose

    Luciferase reporter to measure miR-155 mediated differential repression. Contains LMBRD2 3' UTR and mutated miR-155 sites

    Depositor

    David Bartel

    Article

    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

    Insert

    LMBRD2 3'UTR and mutated miR-155 binding site (LMBRD2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    mutated miR-155 binding site

    Promoter

    thymidine kinase (HSV-TK promoter)

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRRLNeo-pEF1a-p53ashL344P-mKate2-splitmVenusN

    Plasmid

    #69586

    Purpose

    Expresses mutated p53-L344P tagged with mKate2 and split N-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344P (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - N term

    Expression

    Mammalian

    Mutation

    p53 L344P mutation that prevents dimerization and…

    Promoter

    EF1alpha

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-FLAG-CHD5-Y619E-FRAG

    Plasmid

    #68871

    Purpose

    Mammalian expression of CHD5 histone binding domain (331-672aa) with Y619 mutation

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    331-672aa, Y619E

    Promoter

    CMV

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pMIR-Report-Luc-KLF4-K5S-Mt206B

    Plasmid

    #34600

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    The KLF4 K5S region was mutated at the miR-206 bi…

    Promoter

    CMV

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pENTR2b-ITGB1(YYFF)

    Plasmid

    #215451

    Purpose

    Gateway entry vector with integrin beta1 NPxY mutant (YYFF)

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    ITGB1 (ITGB1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Mutations in the NPxY sites Y783F, Y795F; Silent …

    Promoter

    none

    Available since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. A1-LCD allW D262V

    Plasmid

    #234616

    Purpose

    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.

    Depositor

    Tanja Mittag

    Article

    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

    Insert

    hnRNPA1_LCD_allW D262V (HNRNPA1 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    C-terminal domain (186-320) bearing familial ALS …

    Promoter

    T7

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. A1-LCD allW D262N

    Plasmid

    #234615

    Purpose

    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262N with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.

    Depositor

    Tanja Mittag

    Article

    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

    Insert

    hnRNPA1_LCD_allW D262N (HNRNPA1 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    C-terminal domain (186-320) bearing familial ALS …

    Promoter

    T7

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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