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pECNUS_MP01

Plasmid

#194350

Purpose

CRISPR-Cas9 multiplexing acceptor clone with BpiI cutter

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

pcoCas9, FAST-Red

Expression

Plant

Promoter

RPS5A

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBRC1

Plasmid

#183064

Purpose

Base editing plasmid for Pseudomonas chlororaphis

Depositor

Xuehong Zhang

Article

Yue et al Microb Biotechnol. 2022 Sep;15(9):2324-2

Insert

eSpCas9ppD10A

Use

CRISPR and Synthetic Biology

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pYES2.1-pomSua1

Plasmid

#107446

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Sua1

Tags

V5-His

Expression

Yeast

Mutation

See depositor comments below.

Promoter

GAL1

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pYES2.1-pomMet14

Plasmid

#107451

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met14 (met14 Fission Yeast)

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pYES2.1-pomMet1

Plasmid

#107444

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met1

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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p221z-AtMIR390a

Plasmid

#118388

Purpose

Entry clone containing AtMIR390a backbone (split by ccdB gene). Used to make artificial microRNA construct. For use in plants and compatible with the MultiSite Gateway system.

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

AtMIR390a backbone

Use

RNAi

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pBA789

Plasmid

#186244

Purpose

soc-F HR Donor Vector (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

BBR1 backbone + T4 phage homology

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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peft-3::cas-9::tbb-2 3'UTR

Plasmid

#48960

Purpose

this plasmid contains the codon optimized gene encoding Cas9 for C. elegans.

Depositor

Mario de Bono

Article

Chen et al Nucleic Acids Res. 2013 Sep 5.

Insert

cas9

Use

CRISPR

Promoter

eef-1A.1 (eft-3)

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFA-MNase-CaARG4

Plasmid

#162463

Purpose

DNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaARG4

Depositor

Adnane Sellam

Article

Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

Insert

3FLAG-Mnase

Expression

Yeast

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFGL1258

Plasmid

#116898

Purpose

TetOFF:GFP:CenpA (centromere marker)

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Inserts

CenpA promoter region
CenpA

Use

Fungal expression (in magnaporthe oryzae)

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET16

Plasmid

#107442

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET16 (MET16 Budding Yeast)

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available Since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

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pN-PURO-PTP

Plasmid

#51711

Purpose

contains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

Depositor

Arthur Gunzl

Article

Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

Type

Empty backbone

Use

T. brucei genome integration vectors

Tags

PTP (ProtC-TEV-ProtA)

Available Since

March 19, 2014

Availability

Academic Institutions and Nonprofits only

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pCR1003

Plasmid

#71821

Purpose

Express Streptococcus pyogenes dCas9 (D10A, H840A)

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Insert

T7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)

Use

CRISPR

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pFH113

Plasmid

#128210

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pOsU3-esgRNA

Plasmid

#115629

Purpose

expression esgRNA in rice protoplasts

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

OsU3p-esgRNA

Use

CRISPR

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pNHP

Plasmid

#22500

Depositor

Sergey Kasparov

Article

Coleman et al Physiol Genomics. 2003 Feb 6. 12(3):221-

Insert

gag, pol, tat, rev

Expression

Mammalian

Available Since

March 12, 2010

Availability

Academic Institutions and Nonprofits only
pLSLZ.D.R

Plasmid

#51510

Purpose

Single component LSL vector. Contains 2x35S:Zif268:FokI followed by the us:NPTII template. This sequence is between the LIR and SIR.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

NLS

Promoter

2x35S

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM-sgControl_dual-MTS

Plasmid

#162765

Purpose

Expressing sgControl_dual-MTS

Depositor

Antony K Chen

Article

Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

Insert

sgControl_dual-MTS

Expression

Mammalian

Promoter

U6

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSEVA221TrxA-AID-T7RNAP

Plasmid

#167976

Purpose

Expresses fusion protein TrxA-AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

TrxA-AID-T7RNAP

Tags

T7RNAP and TrxA

Expression

Bacterial

Promoter

Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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PX458_GABPA_2

Plasmid

#64255

Purpose

Expresses gRNA against human GABPA along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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