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Abp1-tDimerRFP

Plasmid

#27699

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Abp1 (Dbnl Mouse)

Use

Tags

tDimer-RFP

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
Unc5c.a-Fc-His

Plasmid

#72180

Purpose

Expresses the extracellular region of the Unc5C, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c.a (Unc5c Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCIG-Lmx1a

Plasmid

#45070

Depositor

Kathleen Millen

Insert

Lmx1a LIM homeobox transcription factor 1 alpha (Lmx1a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 30, 2013

Availability

Academic Institutions and Nonprofits only
mCh-KIF5A*-strep

Plasmid

#120163

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5A (Kif5a Mouse)

Use

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF5A: 1-559 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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attB-mCh-Dnmt3b1-Poly(A)-PuroR

Plasmid

#65556

Purpose

Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Puromycin selection

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Dnmt3b (Dnmt3b Mouse)

Use

Mouse Targeting; Bxb1

Tags

mCherry

Expression

Mammalian

Mutation

isoform 1

Promoter

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-Rosmarinus

Plasmid

#183462

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-rosmarinus fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

Rosmarinus

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-Nedd4-N / 2524 JM16

Plasmid

#13312

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

Nedd4 (Nedd4 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

aa1-52 and aa422-777 deleted

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pHAGE2-TetOminiCMV-SM

Plasmid

#136578

Purpose

Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Sox2-E2A-cMyc (Sox2, Myc Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAav-MCS-PQS2-3xHA

Plasmid

#84917

Purpose

rAAV-based template for genome engineering of protein C-termini containing PQS2 and 3xHA tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Insert

LOX-PGK-NEO-LOX

Use

AAV

Tags

PQS2 3xHA

Expression

Mutation

Promoter

mPGK

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-mGSDMD1-251

Plasmid

#111565

Purpose

Express ELANE-cleaved mouse GSDMD in mammalian cells

Depositor

Hongbo Luo

Article

Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

Insert

Gasdermin D (Gsdmd Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA-Flag-Trib3

Plasmid

#131157

Purpose

Mammalian expression of flag-tagged mouse TRIB3

Depositor

Warren Pear, Karen Keeshan

Article

Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 1

Insert

Trib3 (Trib3 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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3534 pcDNA3 Bax

Plasmid

#8783

Depositor

Stanley Korsmeyer

Article

Oltvai et al Cell 1993 Aug 27;74(4):609-19.

Insert

Bax (Bax Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/Puro/FA/GFP-LL5BIP

Plasmid

#112829

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Article

Niewiadomski et al Matters v. 2, n. 11 (Nov 17, 2016)

Insert

LL5BIP (2410002F23Rik Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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HA-RNF126 C231A

Plasmid

#138648

Purpose

Mammalian expression of full-length2 HA-tagged RNF126 with an inactivating mutation in the RING domain (C231A)

Depositor

Jane McGlade

Article

Smith et al Exp Cell Res. 2014 Jan 15;320(2):219-32.

Insert

RNF126 (RNF126 Mouse)

Use

Tags

HA-HA

Expression

Mammalian

Mutation

C231A

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-deltaCTD-HA.SV40(polyA)

Plasmid

#156163

Purpose

Expresses truncated (AA 1-954) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

Truncated THBS1 (amino acid residues 1-954)

Promoter

CMV

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4

Plasmid

#193348

Purpose

tetO-S*K (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Sox2-17-t2a-Klf4 (Klf4, Sox2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

TetO mini CMV

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-MMP9 matrix metalloproteinase-9 precursor [L51/82R]

Plasmid

#149455

Purpose

Mammalian Expression Plasmid of anti-MMP9 matrix metalloproteinase-9 precursor (Rat). Derived from hybridoma L51/82.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-MMP9 matrix metalloproteinase-9 precursor (Rattus norvegicus) recombinant mouse monoclonal antibody (Mmp9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCXN2-mVIN

Plasmid

#11776

Depositor

Eileen Adamson

Article

Xu et al J Cell Sci. 1998 Jun . 111 ( Pt 11)():15

Insert

vinculin (Vcl Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only
mChT pcDNA3

Plasmid

#15765

Depositor

Randy Blakely

Article

Apparsundaram et al Biochem Soc Trans. 2001 Nov . 29(Pt 6):7

Insert

hemicholinium-3-sensitive choline transporter (Slc5a7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
iDuet101a-mCHF1

Plasmid

#55611

Purpose

Lentivirus containing mouse Hey2 coding region

Depositor

Michael Chin

Article

Hartman et al PLoS One. 2014 Mar 25;9(3):e93033. doi:

Insert

Hey2 (Hey2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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pEBG-MKK4-S255E/T259D (GST)

Plasmid

#21565

Depositor

Leonard Zon

Article

Sanchez et al Nature. . 372(6508):794-8.

Insert

mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

Serine 255 to Glutamic Acid, Threonine 259 to As…

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA-ELYS-emerin-EGFP-polyA

Plasmid

#59745

Purpose

Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

ELYS-emerin-EGFP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pBS Notch4HA

Plasmid

#21470

Depositor

Jan Kitajewski

Article

Uyttendaele et al Dev Biol. 1998 Apr 15. 196(2):204-17.

Insert

Notch4 (Notch4 Mouse)

Use

Cloning vector

Tags

HA

Expression

Mutation

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
Fgf4enhLV (FpG12)

Plasmid

#69444

Purpose

positive control plasmid for lentiviral reporter expression in ESCs

Depositor

Lisa Dailey

Article

Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

Inserts

minimal FGF4 promoter
EGFP
Ubiquitin promoter
Hygromycin resistance
FGF4 enhancer (Fgf4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-Prom1 SV8

Plasmid

#184011

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286).

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Wild type cDNA

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBICD2-TUG

Plasmid

#52873

Purpose

retroviral expression of TUG

Depositor

Jonathan Bogan

Article

Bogan et al Nature. 2003 Oct 16;425(6959):727-33.

Insert

TUG (Aspscr1 Mouse)

Use

Retroviral

Tags

IRES-CD2 (mouse)

Expression

Mammalian

Mutation

Promoter

retrovirus LTR

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-mCh-Oct4i

Plasmid

#21906

Depositor

Miguel Ramalho-Santos

Article

Gaspar-Maia et al Nature. 2009 Jul 8. ():.

Insert

Oct4i

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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MSCV KSR1 IRES GFP

Plasmid

#25973

Depositor

Rob Lewis

Article

Kortum et al Mol Cell Biol. 2004 May . 24(10):4407-16

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
CBP-HAT-pET

Plasmid

#21091

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

Tags

His and T7 tag

Expression

Bacterial

Mutation

HAT domain aa 1171-1621

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
Jam1-AP-His

Plasmid

#71952

Purpose

Expresses the extracellular region of the JAM-1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam1 (F11r Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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