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We narrowed to 10,975 results for: NSI;

Showing: 6321 - 6360 of 10975 results

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pCA528 SNX15 MIT residues 263-342

Plasmid

#180625

Purpose

Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-SUMO tag. Internal ID:WISP21-14

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SNX15 (SNX15 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 263-342

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 SPASTIN , F124D, residues 108-200

Plasmid

#180629

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has F124D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-12.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200, F124D

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 SPASTIN , L177D, residues 108-200

Plasmid

#180634

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200, L177D

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 USP8, residues 1-147

Plasmid

#180636

Purpose

Bacterial expression for USP8 MIT domain, residues 1-147. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-86.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

USP8 (USP8 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-147

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 USP54, residues 731-878

Plasmid

#180637

Purpose

Bacterial expression for USP54 MIT domain, residues 731-878. Has N-terminal HIS-SUMO tag. Internal ID:WISP20-85

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

USP54 (USP54 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET16B VPS4A, residues 1-84

Plasmid

#180638

Purpose

Bacterial expression for VPS4A MIT domain residues 1-84. Has N-terminal HIS-TAG. Internal ID: WISP05-43

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

VPS4A (VPS4A Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

Residues 1-84

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CALPAIN-7 residues 1-165, V18D

Plasmid

#180612

Purpose

Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has V18D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-26.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-165, V18D mutation

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CALPAIN-7 residues 1-165, F98D

Plasmid

#180613

Purpose

Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has F98D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-27.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-165, F98D mutation

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-GSDMD_C39A-Myc

Plasmid

#201167

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Expression

Mutation

C39A

Promoter

MESV

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3

Plasmid

#197426

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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hTln1-R1R2

Plasmid

#191440

Purpose

Expresses the human TLN1 R1R2 domains in bacteria

Depositor

Ben Goult

Article

Gallego-Paez et al J Cell Biol. 2023 May 1;222(5):e20220901

Insert

Human Talin1 R1R2 domains (TLN1 Human)

Use

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)]-Fluc

Plasmid

#200127

Purpose

Retroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)] protease reporter and P2A firefly luciferase in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)] P2A firefly luciferase

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(SA)]-Fluc

Plasmid

#200128

Purpose

Retroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(SA)] protease reporter and P2A firefly luciferase in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[CoVA(SA)] P2A firefly luciferase

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc

Plasmid

#200126

Purpose

Retroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[CoVA] P2A firefly luciferase

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG.Myc-SARS2_3CL(C145A)

Plasmid

#200121

Purpose

Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL C145A in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)

Use

Tags

Myc

Expression

Mammalian

Mutation

C145A

Promoter

CAG

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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hPCNA-C148S

Plasmid

#190939

Purpose

untagged human PCNA with a cysteine to serine mutation

Depositor

Brian Kelch

Article

Magrino et al J Biol Chem. 2023 May;299(5):104656. doi

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Use

Tags

Expression

Bacterial

Mutation

changed cysteine 148 to serine

Promoter

T7

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmMextli_577-620_AB

Plasmid

#148337

Purpose

Insect Expression of DmMextli_577-520

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli_577-520 (mxt Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmMextli_577-640_AB

Plasmid

#148338

Purpose

Insect Expression of DmMextli_577-640

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli_577-640 (mxt Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4E-N110EH111E-siRNAres_AB

Plasmid

#148339

Purpose

Insect Expression of DmeIF4E-N110EH111E-siRNAres

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmeIF4E-N110EH111E-siRNAres

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-CeeIF4G_315-491opt_AB

Plasmid

#148349

Purpose

Bacterial Expression of CeeIF4G_315-491opt

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeeIF4G_315-491opt (ifg-1 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-CeMextli_471-507opt_AB

Plasmid

#148350

Purpose

Bacterial Expression of CeMextli_471-507opt

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeMextli_471-507opt (Y18D10A.8 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-CeMextli_471-507opt-GB1_AB

Plasmid

#148351

Purpose

Bacterial Expression of CeMextli_471-507opt

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeMextli_471-507opt (Y18D10A.8 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-CeMextli_471-507opt-I497DI504D-GB1_AB

Plasmid

#148352

Purpose

Bacterial Expression of CeMextli_471-507opt-I497DI504D

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeMextli_471-507opt-I497DI504D (Y18D10A.8 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-CeMextli_471-507opt-M493D-GB1_AB

Plasmid

#148353

Purpose

Bacterial Expression of CeMextli_471-507opt-M493D

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeMextli_471-507opt-M493D (Y18D10A.8 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-CeMextli_471-507opt-Y479AL484AM485A-GB1_AB

Plasmid

#148354

Purpose

Bacterial Expression of CeMextli_471-507opt-Y479AL484AM485A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeMextli_471-507opt-Y479AL484AM485A (Y18D10A.8 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-DmMextli_577-620_AB

Plasmid

#148355

Purpose

Bacterial Expression of DmMextli_577-620

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli_577-620 (mxt Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-DmMextli_577-640_AB

Plasmid

#148360

Purpose

Bacterial Expression of DmMextli_577-640

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli_577-640 (mxt Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpH-CeeIF4E3_1-215opt-V58AI74A_AB

Plasmid

#148368

Purpose

Bacterial Expression of CeeIF4E3_1-215-V58AI74A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeeIF4E3_1-215-V58AI74A

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
DsRed -7e

Plasmid

#191765

Purpose

Expression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7e

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

DsRed

Use

Tags

N terminal fusion of negatively charged polypetod…

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRD424

Plasmid

#178202

Purpose

Expression of Rok from the hns promoter

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

phns + rok

Use

Tags

Expression

Bacterial

Mutation

Promoter

hns promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-3x-NLS-mScarlet-AMPKα2

Plasmid

#192452

Purpose

mScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)

Use

Tags

3x Nuclear localization signal (NLS) and mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBeast-pT7-lox

Plasmid

#190059

Purpose

Express the bacterial lactate oxidase enzyme under the control of the strong constitutif phage promoter T7

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

lox

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBeast-pT7-codA

Plasmid

#190058

Purpose

Express the bacterial choline oxidase enzyme under the control of the strong constitutif phage promoter T7

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

codA

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBeast-pYjjZ-RBS- sfGFP

Plasmid

#190054

Purpose

GFP containing reporter plasmid expressing it under the control of the reportedely OxyR/H2O2 inducible pYjjZ promoter

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBeast-pKatG-RBS- sfGFP

Plasmid

#190053

Purpose

GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pKatG promoter

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBeast-pOxyS-RBS- sfGFP

Plasmid

#190052

Purpose

GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pOxyS promoter

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRD410

Plasmid

#178200

Purpose

Expression of sRok from the hns promoter

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

phns + srok

Use

Tags

Expression

Bacterial

Mutation

Promoter

hns promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NK10 SFFV-mCherry-SG(s70587-3xMS2)SG-EGFP (FLP-IN)

Plasmid

#191165

Purpose

Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the coding sequence in mammalian cells. Similar to EK0438, but with MS2 sequences directly after the sensor sequence, rather than 3' UTR

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587-3xMS2:EGFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
EK0312 pAAV-EF1a-DIO-EYFP-WPRE-HGHpA

Plasmid

#191148

Purpose

AAV expression of a Cre reporter with EYFP output; derived from a gift from prof. Liqun Luo

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

DIO-EYFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

hEF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pACE-GIRK2-mC-S

Plasmid

#172428

Purpose

Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK2 (KCNJ6 Human)

Use

Tags

mCherry Streptag II

Expression

Insect

Mutation

Promoter

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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